Comparison of Carbohydrate Compositions of Total Apolipoproteins in Lipoproteins

Tayfun GÜLDÜR; Sema OZAN; Tülay İLERİ

Makale detayları

Comparison of Carbohydrate Compositions of Total Apolipoproteins in Lipoproteins

Yazarlar:

Tayfun GÜLDÜR | Sema OZAN | Tülay İLERİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 1999 Vol.23 Issue EK4, pp.777-784

Belge Tipi:

Makale

Yayın Tarihi:

11/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000772315

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Terminal carbohydrate moieties of apolipoproteins of lipoproteins in human and goat serum were ascertained and compared. Apolipoproteins of b+pre-b (apolipoprotein B containing lipoproteins) and a lipoproteins separated by phosphotungstic acid/MgCl2 precipitation method were applied to SDS-PAGE and blotted onto nitrocellulose membrane. Digoxigenin labelled lectins, each of which recognizes a specific sugar sequence, were incubated with apolipoproteins immobilized on a western blot membrane to probe some of carbohydrate structures commonly found in carbohydrate chains of glycoproteins.The presence of bound lectin on the membrane was detected with alkaline phosphatase labelled antidigoxigenin antibody.Sialic acid terminally linked a (2--3) to galactose was detected on the largest protein band in b+pre-b lipoprotein fraction of both human and goat serum with the mobility identical to that of apo B.Neither of the carbohydrate moieties probed were found to be present in a lipoproteins.

 

Terminal carbohydrate moieties of apolipoproteins of lipoproteins in human and goat serum were ascertained and compared. Apolipoproteins of b+pre-b (apolipoprotein B containing lipoproteins) and a lipoproteins separated by phosphotungstic acid/MgCl2 precipitation method were applied to SDS-PAGE and blotted onto nitrocellulose membrane. Digoxigenin labelled lectins, each of which recognizes a specific sugar sequence, were incubated with apolipoproteins immobilized on a western blot membrane to probe some of carbohydrate structures commonly found in carbohydrate chains of glycoproteins.The presence of bound lectin on the membrane was detected with alkaline phosphatase labelled antidigoxigenin antibody.Sialic acid terminally linked a (2--3) to galactose was detected on the largest protein band in b+pre-b lipoprotein fraction of both human and goat serum with the mobility identical to that of apo B.Neither of the carbohydrate moieties probed were found to be present in a lipoproteins.

Makale detayları

Comparison of Carbohydrate Compositions of Total Apolipoproteins in Lipoproteins

Yazarlar:

Tayfun GÜLDÜR | Sema OZAN | Tülay İLERİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 1999 Vol.23 Issue EK4, pp.777-784

Belge Tipi:

Makale

Yayın Tarihi:

11/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000772315

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Lipoprotein

apolipoprotein

carbohydrate moiety

Sayı

Makale detayları

Comparison of Carbohydrate Compositions of Total Apolipoproteins in Lipoproteins

Yazarlar: Tayfun GÜLDÜR | Sema OZAN | Tülay İLERİ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 1999 Vol.23 Issue EK4, pp.777-784

Belge Tipi: Makale

Yayın Tarihi: 11/1998

Belge Dili: Türkçe

Document ID (Org No.): 2000772315

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Lipoprotein

apolipoprotein

carbohydrate moiety

Sayı

Yazarlar:

Tayfun GÜLDÜR | Sema OZAN | Tülay İLERİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 1999 Vol.23 Issue EK4, pp.777-784

Belge Tipi:

Makale

Yayın Tarihi:

11/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000772315

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128