The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells

Selda Güler Atmaca; Banu Aydın; Hulya Cabadak

Makale detayları

The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells

Yazarlar:

Selda Güler Atmaca | Banu Aydın | Hulya Cabadak

Yayıncı:

Marmara University

Ana Başlıklar:

Surgery (Other)

Dergi:

Marmara Medical Journal Vol.37 Issue 1, pp.86-91

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001302660

pISSN:

1019-1941

Anahtar kelimeler:

Özet:

 Objective: Muscarinic receptors M1-M5 are members of the G protein-coupled receptor superfamily and are effective in physiological functions through G proteins. Recent studies suggested that cholinergic receptors mediate cellular activities in hematopoietic cells. The aim of this study was to investigate the potential role of mitogen-activated protein kinases MAPK signaling extracellular signalregulated kinases 1 and 2 ERK12phosphorylated ERK1 pERK12 pathways in chronic myeloid leukemia K562 cells. Materials and Methods: Chronic myeloid leukemia cells were cultured. Cells were incubated in the presence of muscarinic receptor agonist, antagonist and epidermal growth factor EGF. To detect MAPK activation, ERKpERK protein expression levels were determined by western blot method techniques. Results: Our study results showed that cholinergic agents and EGFs affect the MAPK pathway in the human K562 cell line. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.

 Objective: Muscarinic receptors M1-M5 are members of the G protein-coupled receptor superfamily and are effective in physiological functions through G proteins. Recent studies suggested that cholinergic receptors mediate cellular activities in hematopoietic cells. The aim of this study was to investigate the potential role of mitogen-activated protein kinases MAPK signaling extracellular signalregulated kinases 1 and 2 ERK12phosphorylated ERK1 pERK12 pathways in chronic myeloid leukemia K562 cells. Materials and Methods: Chronic myeloid leukemia cells were cultured. Cells were incubated in the presence of muscarinic receptor agonist, antagonist and epidermal growth factor EGF. To detect MAPK activation, ERKpERK protein expression levels were determined by western blot method techniques. Results: Our study results showed that cholinergic agents and EGFs affect the MAPK pathway in the human K562 cell line. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.

 Objective: Muscarinic receptors M1-M5 are members of the G protein-coupled receptor superfamily and are effective in physiological functions through G proteins. Recent studies suggested that cholinergic receptors mediate cellular activities in hematopoietic cells. The aim of this study was to investigate the potential role of mitogen-activated protein kinases MAPK signaling extracellular signalregulated kinases 1 and 2 ERK12phosphorylated ERK1 pERK12 pathways in chronic myeloid leukemia K562 cells. Materials and Methods: Chronic myeloid leukemia cells were cultured. Cells were incubated in the presence of muscarinic receptor agonist, antagonist and epidermal growth factor EGF. To detect MAPK activation, ERKpERK protein expression levels were determined by western blot method techniques. Results: Our study results showed that cholinergic agents and EGFs affect the MAPK pathway in the human K562 cell line. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.

 Objective: Muscarinic receptors M1-M5 are members of the G protein-coupled receptor superfamily and are effective in physiological functions through G proteins. Recent studies suggested that cholinergic receptors mediate cellular activities in hematopoietic cells. The aim of this study was to investigate the potential role of mitogen-activated protein kinases MAPK signaling extracellular signalregulated kinases 1 and 2 ERK12phosphorylated ERK1 pERK12 pathways in chronic myeloid leukemia K562 cells. Materials and Methods: Chronic myeloid leukemia cells were cultured. Cells were incubated in the presence of muscarinic receptor agonist, antagonist and epidermal growth factor EGF. To detect MAPK activation, ERKpERK protein expression levels were determined by western blot method techniques. Results: Our study results showed that cholinergic agents and EGFs affect the MAPK pathway in the human K562 cell line. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.

Makale detayları

The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells

Yazarlar:

Selda Güler Atmaca | Banu Aydın | Hulya Cabadak

Yayıncı:

Marmara University

Ana Başlıklar:

Surgery (Other)

Dergi:

Marmara Medical Journal Vol.37 Issue 1, pp.86-91

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001302660

pISSN:

1019-1941

G protein coupled receptor

Carbachol

Epidermal growth factor

Mitogen-activated protein kinase

Chronic myeloid leukemia

Sayı

Makale detayları

The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells

Yazarlar: Selda Güler Atmaca | Banu Aydın | Hulya Cabadak

Yayıncı: Marmara University

Ana Başlıklar: Surgery (Other)

Dergi: Marmara Medical Journal Vol.37 Issue 1, pp.86-91

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001302660

pISSN: 1019-1941

G protein coupled receptor

Carbachol

Epidermal growth factor

Mitogen-activated protein kinase

Chronic myeloid leukemia

Sayı

Yazarlar:

Selda Güler Atmaca | Banu Aydın | Hulya Cabadak

Yayıncı:

Marmara University

Ana Başlıklar:

Surgery (Other)

Dergi:

Marmara Medical Journal Vol.37 Issue 1, pp.86-91

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001302660

pISSN:

1019-1941