Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria

Olawale Olufemi ADELOWO; Obasola Ezekiel FAGADE

Makale detayları

Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria

Yazarlar:

Olawale Olufemi ADELOWO | Obasola Ezekiel FAGADE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 1, pp.37-45

Belge Tipi:

Makale

Yayın Tarihi:

12/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812706

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics use in livestock production. The present study investigated the pattern and mechanisms of resistance in 21 bacteria species isolated from a river used as a waste-dump by a poultry farm in Ogbomoso, southwestern Nigeria. Bacteria were isolated on nutrient agar supplemented with 50 µg/mL of tetracycline, their identities were determined by PCR amplification, and sequencing of their 16s rRNA genes and their susceptibility to 6 antimicrobials determined by broth dilution. The molecular basis of resistance among the isolates was also investigated by the PCR amplification of class 1 and 2 integrons, tetA, tetB, tetC, tetD, tetE, tetG, tetL, tetW, tetM, tet37, blaTEM, aadA1, strA, strB, sul2, sul3 and a 550bp gene fragment encoding a multidrug resistance efflux transporter of the resistance nodulation division (RND) family. All the isolates were resistant to the tested antimicrobials with MIC ranging from.

 

Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics use in livestock production. The present study investigated the pattern and mechanisms of resistance in 21 bacteria species isolated from a river used as a waste-dump by a poultry farm in Ogbomoso, southwestern Nigeria. Bacteria were isolated on nutrient agar supplemented with 50 µg/mL of tetracycline, their identities were determined by PCR amplification, and sequencing of their 16s rRNA genes and their susceptibility to 6 antimicrobials determined by broth dilution. The molecular basis of resistance among the isolates was also investigated by the PCR amplification of class 1 and 2 integrons, tetA, tetB, tetC, tetD, tetE, tetG, tetL, tetW, tetM, tet37, blaTEM, aadA1, strA, strB, sul2, sul3 and a 550bp gene fragment encoding a multidrug resistance efflux transporter of the resistance nodulation division (RND) family. All the isolates were resistant to the tested antimicrobials with MIC ranging from.

Makale detayları

Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria

Yazarlar:

Olawale Olufemi ADELOWO | Obasola Ezekiel FAGADE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 1, pp.37-45

Belge Tipi:

Makale

Yayın Tarihi:

12/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812706

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: 16s rRNA

resistance genes

RND transporter

integrons

Sayı

Makale detayları

Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria

Yazarlar: Olawale Olufemi ADELOWO | Obasola Ezekiel FAGADE

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2012 Vol.36 Issue 1, pp.37-45

Belge Tipi: Makale

Yayın Tarihi: 12/2011

Belge Dili: İngilizce

Document ID (Org No.): 2000812706

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: 16s rRNA

resistance genes

RND transporter

integrons

Sayı

Yazarlar:

Olawale Olufemi ADELOWO | Obasola Ezekiel FAGADE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 1, pp.37-45

Belge Tipi:

Makale

Yayın Tarihi:

12/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812706

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152