A Comparative Analysis of Perinatal Development of Barrel Cortex in Rat, Mouse and Guinea Pig Using Acetylcholinesterase Histochemistry

Erdoğan ŞENDEMİR

Makale detayları

A Comparative Analysis of Perinatal Development of Barrel Cortex in Rat, Mouse and Guinea Pig Using Acetylcholinesterase Histochemistry

Yazarlar:

Erdoğan ŞENDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 4, pp.327-332

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757570

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

The role of acetylcholinesterase  (AChE) in the developing neocortex was  reexamined by comparing its expression in  rats, mice and guinea pigs, following the  protocol for acetylcholinesterase  histochemistry (described in Sendemir et al.,  1996) in order to determine the suitability of  the breeding colony at UludaÛ University for  use as an animal model.  A total of 103 pups as well as two adult  animals of each species were used.  In the rat pups, acetylcholinesterase-rich  patches were distributed in a vibrissa-related  array in the somatosensory cortex soon after  birth, whereas regions of the cortex lying  between individual patches and between  rows of patches in the were depleted  enzyme. Mice on postnatal day 3 and older  mice revealed lightly stained,  acetylcholinesterase positive spots in the  center of barrel cores, while the barrel walls  remained devoid of acetylcholinesterase;  septae that divided the individual barrels  were densely enzyme positive. The barrels in  the guinea pigs formed before birth. Well-  formed barrels were observed by postnatal  day 0, and acetylcholinesterase activity  gradually decreased by postnatal day 10 but  did not fade away. The differential enzyme  location in different rodents indicates that its  role in the development of thalamocortical  connectivity is distinctly different in rats as  opposed to mice and guinea pigs.

 

The role of acetylcholinesterase  (AChE) in the developing neocortex was  reexamined by comparing its expression in  rats, mice and guinea pigs, following the  protocol for acetylcholinesterase  histochemistry (described in Sendemir et al.,  1996) in order to determine the suitability of  the breeding colony at UludaÛ University for  use as an animal model.  A total of 103 pups as well as two adult  animals of each species were used.  In the rat pups, acetylcholinesterase-rich  patches were distributed in a vibrissa-related  array in the somatosensory cortex soon after  birth, whereas regions of the cortex lying  between individual patches and between  rows of patches in the were depleted  enzyme. Mice on postnatal day 3 and older  mice revealed lightly stained,  acetylcholinesterase positive spots in the  center of barrel cores, while the barrel walls  remained devoid of acetylcholinesterase;  septae that divided the individual barrels  were densely enzyme positive. The barrels in  the guinea pigs formed before birth. Well-  formed barrels were observed by postnatal  day 0, and acetylcholinesterase activity  gradually decreased by postnatal day 10 but  did not fade away. The differential enzyme  location in different rodents indicates that its  role in the development of thalamocortical  connectivity is distinctly different in rats as  opposed to mice and guinea pigs.

Makale detayları

A Comparative Analysis of Perinatal Development of Barrel Cortex in Rat, Mouse and Guinea Pig Using Acetylcholinesterase Histochemistry

Yazarlar:

Erdoğan ŞENDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 4, pp.327-332

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757570

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Thalamocortical development

barrel cortex

rodent

and acetylcholinesterase histochemistry.

Sayı

Makale detayları

A Comparative Analysis of Perinatal Development of Barrel Cortex in Rat, Mouse and Guinea Pig Using Acetylcholinesterase Histochemistry

Yazarlar: Erdoğan ŞENDEMİR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2000 Vol.30 Issue 4, pp.327-332

Belge Tipi: Makale

Yayın Tarihi: 3/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000757570

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Thalamocortical development

barrel cortex

rodent

and acetylcholinesterase histochemistry.

Sayı

Yazarlar:

Erdoğan ŞENDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 4, pp.327-332

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757570

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144