A Study on Cellulolytic and Hemicellulolytic Enzymes of Anaerobic Rumen Bacterium Ruminococcus flavefaciens Strain 17

M. Sait EKİNCİ

Makale detayları

A Study on Cellulolytic and Hemicellulolytic Enzymes of Anaerobic Rumen Bacterium Ruminococcus flavefaciens Strain 17

Yazarlar:

M. Sait EKİNCİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 5, pp.703-709

Belge Tipi:

Makale

Yayın Tarihi:

4/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771156

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Polysaccharide-degrading enzymes of the anaerobic rumen bacterium Ruminococcus flavefaciens strain 17 were exmined. This work demonstrated that R. flavefaciens strain 17 produces a complex array of enzymes active against cellulose, hemicellulose (xylan) and lignin. These enzymes are xylanase, avicellase, acid-swollen cellulase, carboxymethyl cellulase (CMCase), pNP-cellobiosidase (pNPC), pNP-glucopyranosidase (pNPG), pNP-xylopyranosidase (pNPX) and pNP-arabinofuranosidase (pNPA). Although R. flavefaciens is an anaerobic bacterium, its enzymes worked optimally under aerobic conditions and with dithiothreitol (DTT), except in the case of pNP-xylopyranosidase. pNP-xylopyranosidase exhibited a higher level of activity under anaerobic conditions with DTT. It was demonstrated that the cross-links between arabinose side chains, which esterified via ferulic acid and p-coumaric acid, were cleaved by R. flavefaciens esterase and a-arabinofuranosidase.

 

Polysaccharide-degrading enzymes of the anaerobic rumen bacterium Ruminococcus flavefaciens strain 17 were exmined. This work demonstrated that R. flavefaciens strain 17 produces a complex array of enzymes active against cellulose, hemicellulose (xylan) and lignin. These enzymes are xylanase, avicellase, acid-swollen cellulase, carboxymethyl cellulase (CMCase), pNP-cellobiosidase (pNPC), pNP-glucopyranosidase (pNPG), pNP-xylopyranosidase (pNPX) and pNP-arabinofuranosidase (pNPA). Although R. flavefaciens is an anaerobic bacterium, its enzymes worked optimally under aerobic conditions and with dithiothreitol (DTT), except in the case of pNP-xylopyranosidase. pNP-xylopyranosidase exhibited a higher level of activity under anaerobic conditions with DTT. It was demonstrated that the cross-links between arabinose side chains, which esterified via ferulic acid and p-coumaric acid, were cleaved by R. flavefaciens esterase and a-arabinofuranosidase.

Makale detayları

A Study on Cellulolytic and Hemicellulolytic Enzymes of Anaerobic Rumen Bacterium Ruminococcus flavefaciens Strain 17

Yazarlar:

M. Sait EKİNCİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 5, pp.703-709

Belge Tipi:

Makale

Yayın Tarihi:

4/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771156

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Ruminococcus flavefaciens

polysaccharidase

esterase

enzymes

Sayı

Makale detayları

A Study on Cellulolytic and Hemicellulolytic Enzymes of Anaerobic Rumen Bacterium Ruminococcus flavefaciens Strain 17

Yazarlar: M. Sait EKİNCİ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 5, pp.703-709

Belge Tipi: Makale

Yayın Tarihi: 4/2001

Belge Dili: Türkçe

Document ID (Org No.): 2000771156

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Ruminococcus flavefaciens

polysaccharidase

esterase

enzymes

Sayı

Yazarlar:

M. Sait EKİNCİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 5, pp.703-709

Belge Tipi:

Makale

Yayın Tarihi:

4/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771156

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128