Effects of Melatonin on FSH/LH/Testosterone and StAR/P450scc/17β-HSD3 Pathways in Acrylamide-Induced Testicular Toxication in Rats

Samet Tekin; Yusuf Dağ; Furkan Aykurt; Merve Bolat; İsmail Bolat; Burak Batuhan Laçin; Burak Çınar; Mohamad Warda

Makale detayları

Effects of Melatonin on FSH/LH/Testosterone and StAR/P450scc/17β-HSD3 Pathways in Acrylamide-Induced Testicular Toxication in Rats

Yazarlar:

Samet Tekin | Yusuf Dağ | Furkan Aykurt | Merve Bolat | İsmail Bolat | Burak Batuhan Laçin | Burak Çınar | Mohamad Warda

Yayıncı:

Ataturk University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.5 Issue 2, pp.119-125

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358092

pISSN:

2791-8645

Anahtar kelimeler:

Özet:

 Acrylamide ACR is a toxic compound formed during high-temperature food processing that is known to induce oxidative stress and reproductive toxicity. This study investigated the protective effects of melatonin MEL on ACR-induced testicular toxicity, with a focus on hormonal regulation and steroidogenic pathways. Forty adults male Wistar rats were divided into five groups: control, ACR 50 mgkg, ACRMEL10 ACR  10 mgkg MEL, ACRMEL20 ACR  20 mgkg MEL, and MEL20 20 mgkg MEL alone. Treatments were administered via intragastric gavage for 14 days. Serum follicle-stimulating hormone FSH, luteinizing hormone LH, and testosterone levels were measured via ELISA, alongside testicular steroidogenic enzyme levels StAR, P450scc, 17β-HSD3. Histopathological analysis was used to assess spermatogenic integrity via Johnsen scoring. ACR exposure significantly reduced the serum FSH, LH, and testosterone levels p .001, with a concurrent decrease in StAR, P450scc, and 17β-HSD3 expression. Histological evaluation revealed severe spermatocyte degeneration, necrosis, and disrupted spermatogenesis. MEL administration ameliorated these effects in a dose-dependent manner. ACRMEL10 resulted in partial restoration of hormonal and enzymatic levels p .05, whereas ACRMEL20 restored these parameters to near-control levels p .05. MEL also mitigated histopathological damage, preserving seminiferous tubule integrity and spermatogenic function. These findings suggest that MEL exerts protective effects against ACR-induced testicular toxicity through its antioxidant and antiapoptotic effects, restoring steroidogenic enzyme activity and the hormonal balance. MEL supplementation may be a potential therapeutic strategy against environmental toxicants affecting male reproductive health. Further mechanistic studies are warranted to elucidate its full protective scope.

 Acrylamide ACR is a toxic compound formed during high-temperature food processing that is known to induce oxidative stress and reproductive toxicity. This study investigated the protective effects of melatonin MEL on ACR-induced testicular toxicity, with a focus on hormonal regulation and steroidogenic pathways. Forty adults male Wistar rats were divided into five groups: control, ACR 50 mgkg, ACRMEL10 ACR  10 mgkg MEL, ACRMEL20 ACR  20 mgkg MEL, and MEL20 20 mgkg MEL alone. Treatments were administered via intragastric gavage for 14 days. Serum follicle-stimulating hormone FSH, luteinizing hormone LH, and testosterone levels were measured via ELISA, alongside testicular steroidogenic enzyme levels StAR, P450scc, 17β-HSD3. Histopathological analysis was used to assess spermatogenic integrity via Johnsen scoring. ACR exposure significantly reduced the serum FSH, LH, and testosterone levels p .001, with a concurrent decrease in StAR, P450scc, and 17β-HSD3 expression. Histological evaluation revealed severe spermatocyte degeneration, necrosis, and disrupted spermatogenesis. MEL administration ameliorated these effects in a dose-dependent manner. ACRMEL10 resulted in partial restoration of hormonal and enzymatic levels p .05, whereas ACRMEL20 restored these parameters to near-control levels p .05. MEL also mitigated histopathological damage, preserving seminiferous tubule integrity and spermatogenic function. These findings suggest that MEL exerts protective effects against ACR-induced testicular toxicity through its antioxidant and antiapoptotic effects, restoring steroidogenic enzyme activity and the hormonal balance. MEL supplementation may be a potential therapeutic strategy against environmental toxicants affecting male reproductive health. Further mechanistic studies are warranted to elucidate its full protective scope.

 Acrylamide ACR is a toxic compound formed during high-temperature food processing that is known to induce oxidative stress and reproductive toxicity. This study investigated the protective effects of melatonin MEL on ACR-induced testicular toxicity, with a focus on hormonal regulation and steroidogenic pathways. Forty adults male Wistar rats were divided into five groups: control, ACR 50 mgkg, ACRMEL10 ACR  10 mgkg MEL, ACRMEL20 ACR  20 mgkg MEL, and MEL20 20 mgkg MEL alone. Treatments were administered via intragastric gavage for 14 days. Serum follicle-stimulating hormone FSH, luteinizing hormone LH, and testosterone levels were measured via ELISA, alongside testicular steroidogenic enzyme levels StAR, P450scc, 17β-HSD3. Histopathological analysis was used to assess spermatogenic integrity via Johnsen scoring. ACR exposure significantly reduced the serum FSH, LH, and testosterone levels p .001, with a concurrent decrease in StAR, P450scc, and 17β-HSD3 expression. Histological evaluation revealed severe spermatocyte degeneration, necrosis, and disrupted spermatogenesis. MEL administration ameliorated these effects in a dose-dependent manner. ACRMEL10 resulted in partial restoration of hormonal and enzymatic levels p .05, whereas ACRMEL20 restored these parameters to near-control levels p .05. MEL also mitigated histopathological damage, preserving seminiferous tubule integrity and spermatogenic function. These findings suggest that MEL exerts protective effects against ACR-induced testicular toxicity through its antioxidant and antiapoptotic effects, restoring steroidogenic enzyme activity and the hormonal balance. MEL supplementation may be a potential therapeutic strategy against environmental toxicants affecting male reproductive health. Further mechanistic studies are warranted to elucidate its full protective scope.

 Akrilamid ACR, oksidatif stres ve üreme toksisitesine neden olduğu bilinen yüksek sıcaklıkta gıda işleme sırasında oluşan toksik bir bileşiktir. Bu çalışmada, hormonal düzenleme ve steroidojenik yollara odaklanılarak melatoninin MEL ACR kaynaklı testis toksisitesi üzerindeki koruyucu etkileri araştırılmıştır. 40 yetişkin erkek Wistar sıçanı beş gruba ayrılmıştır: kontrol, ACR 50 mgkg, ACRMEL10 ACR  10 mgkg MEL, ACRMEL20 ACR  20 mgkg MEL ve MEL20 sadece 20 mgkg MEL. Tedaviler 14 gün boyunca intragastrik gavaj yoluyla uygulanmıştır. Serum folikül uyarıcı hormon FSH, luteinize edici hormon LH ve testosteron seviyeleri, testis steroidojenik enzim seviyeleri StAR, P450scc, 17β-HSD3 ile birlikte ELISA yoluyla ölçülmüştür. Histopatolojik analiz, Johnsen puanlaması yoluyla spermatogenik bütünlüğü değerlendirmek için kullanıldı. ACR maruziyeti serum FSH, LH ve testosteron seviyelerini önemli ölçüde düşürdü p .001, StAR, P450scc ve 17β-HSD3 ekspresyonunda eş zamanlı bir azalma oldu. Histolojik değerlendirme şiddetli spermatosit dejenerasyonu, nekroz ve bozulmuş spermatogenez olduğunu ortaya koydu. MEL uygulaması bu etkileri doza bağlı bir şekilde iyileştirdi. ACRMEL10 hormonal ve enzimatik seviyelerin kısmi restorasyonuyla sonuçlandı p .05, oysa ACRMEL20 bu parametreleri kontrol seviyelerine yakın seviyelere geri getirdi p .05. MEL ayrıca histopatolojik hasarı hafifletti, seminifer tübül bütünlüğünü ve spermatogenik işlevi korudu. Bu bulgular, MELin antioksidan ve antiapoptotik etkileriyle ACR kaynaklı testis toksisitesine karşı koruyucu etkiler gösterdiğini, steroidojenik enzim aktivitesini ve hormonal dengeyi geri kazandırdığını göstermektedir. MEL takviyesi, erkek üreme sağlığını etkileyen çevresel toksik maddelere karşı potansiyel bir tedavi stratejisi olabilir. Tam koruyucu kapsamını açıklamak için daha fazla mekanik çalışma yapılması gerekmektedir.

Makale detayları

Effects of Melatonin on FSH/LH/Testosterone and StAR/P450scc/17β-HSD3 Pathways in Acrylamide-Induced Testicular Toxication in Rats

Yazarlar:

Samet Tekin | Yusuf Dağ | Furkan Aykurt | Merve Bolat | İsmail Bolat | Burak Batuhan Laçin | Burak Çınar | Mohamad Warda

Yayıncı:

Ataturk University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.5 Issue 2, pp.119-125

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358092

pISSN:

2791-8645

Keywords: Acrylamide toxicity

Melatonin protection

Oxidative stress

Reproductive toxicity

Testicular steroidogenesisAkrilamid toksisitesi

Melatonin koruması

Oksidatif stres

Testiküler steroidogenez

Üreme toksisitesi.

Sayı

Makale detayları

Yazarlar: Samet Tekin | Yusuf Dağ | Furkan Aykurt | Merve Bolat | İsmail Bolat | Burak Batuhan Laçin | Burak Çınar | Mohamad Warda

Yayıncı: Ataturk University

Ana Başlıklar: Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi: Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.5 Issue 2, pp.119-125

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001358092

pISSN: 2791-8645

Sayı

Yazarlar:

Samet Tekin | Yusuf Dağ | Furkan Aykurt | Merve Bolat | İsmail Bolat | Burak Batuhan Laçin | Burak Çınar | Mohamad Warda

Yayıncı:

Ataturk University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.5 Issue 2, pp.119-125

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358092

pISSN:

2791-8645