Evaluation of distribution and susceptibility of microorganisms isolated from joint fluid cultures: five-year data

Halil Er; Özlem Koca; Nevgün Sepin Özen

Makale detayları

Evaluation of distribution and susceptibility of microorganisms isolated from joint fluid cultures: five-year data

Yazarlar:

Halil Er | Özlem Koca | Nevgün Sepin Özen

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Tıbbi Bakteriyoloji

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.432-437

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302544

pISSN:

2718-0115

Anahtar kelimeler:

Özet:

 Aims: Septic arthritis is joint inflammation. It is an orthopedic emergency that requires prompt diagnosis and treatment. In this study, it was aimed to examine the distribution and antibiotic resistance profiles of microorganisms isolated from joint fluid samples taken from patients who applied to the orthopedic clinic of our hospital in the last five years. Methods: In our study, 1162 joint fluid samples were sent to the medical microbiology laboratory of our hospital from the orthopedics and traumatology clinic between January 01, 2018 and December 31, 2022. Joint fluid samples taken from clinically appropriate patients under sterile conditions were incubated in a BacTAlert 3D Biomerieux, France culture device. Bacteria isolated from 164 specimens with growth detected, were identified by matrix-mediated laser desorptionionization-time-of-flight mass spectrometry MALDI-TOF MS based VITEK MS Biomerieux, France. Antibiotic susceptibility tests were performed on the VITEK 2 Compact Biomerieux, France device. Results: Staphylococcus aureus S. aureus 29.3 and coagulase negative Staphylococci CNS 29.3 were the most commonly grown microorganisms. Other microorganisms grown were Streptococcus spp. 9.1, Enterococcus spp. 6.1, Pseudomonas aeruginosa P. aeuruginosa 7.3, Escherichiae coli E. coli 4.3 and Klebsiella pneumoniae K. pneumoniae 4.3. When antibiotic susceptibility results were evaluated according to EUCAST restricted reporting criteria, linezolid in Gram-positive strains, amikacin in Enterobacterales, colistin and tigecycline in nonfermentative Gram-negative bacteria were found to be the most susceptible antibiotics. Conclusion: The continuous change in antibiotic susceptibility profiles in joint infections, the long duration of treatment and follow-up, and the increase in polymicrobial infections require regular monitoring of culture and antibiotic susceptibility tests. In our study, the distribution of microorganisms isolated from joint fluid samples of our hospital and the determination of antibiotic resistance profiles will contribute to the clinician in terms of guiding empirical treatment.

 Aims: Septic arthritis is joint inflammation. It is an orthopedic emergency that requires prompt diagnosis and treatment. In this study, it was aimed to examine the distribution and antibiotic resistance profiles of microorganisms isolated from joint fluid samples taken from patients who applied to the orthopedic clinic of our hospital in the last five years. Methods: In our study, 1162 joint fluid samples were sent to the medical microbiology laboratory of our hospital from the orthopedics and traumatology clinic between January 01, 2018 and December 31, 2022. Joint fluid samples taken from clinically appropriate patients under sterile conditions were incubated in a BacTAlert 3D Biomerieux, France culture device. Bacteria isolated from 164 specimens with growth detected, were identified by matrix-mediated laser desorptionionization-time-of-flight mass spectrometry MALDI-TOF MS based VITEK MS Biomerieux, France. Antibiotic susceptibility tests were performed on the VITEK 2 Compact Biomerieux, France device. Results: Staphylococcus aureus S. aureus 29.3 and coagulase negative Staphylococci CNS 29.3 were the most commonly grown microorganisms. Other microorganisms grown were Streptococcus spp. 9.1, Enterococcus spp. 6.1, Pseudomonas aeruginosa P. aeuruginosa 7.3, Escherichiae coli E. coli 4.3 and Klebsiella pneumoniae K. pneumoniae 4.3. When antibiotic susceptibility results were evaluated according to EUCAST restricted reporting criteria, linezolid in Gram-positive strains, amikacin in Enterobacterales, colistin and tigecycline in nonfermentative Gram-negative bacteria were found to be the most susceptible antibiotics. Conclusion: The continuous change in antibiotic susceptibility profiles in joint infections, the long duration of treatment and follow-up, and the increase in polymicrobial infections require regular monitoring of culture and antibiotic susceptibility tests. In our study, the distribution of microorganisms isolated from joint fluid samples of our hospital and the determination of antibiotic resistance profiles will contribute to the clinician in terms of guiding empirical treatment.

 Aims: Septic arthritis is joint inflammation. It is an orthopedic emergency that requires prompt diagnosis and treatment. In this study, it was aimed to examine the distribution and antibiotic resistance profiles of microorganisms isolated from joint fluid samples taken from patients who applied to the orthopedic clinic of our hospital in the last five years. Methods: In our study, 1162 joint fluid samples were sent to the medical microbiology laboratory of our hospital from the orthopedics and traumatology clinic between January 01, 2018 and December 31, 2022. Joint fluid samples taken from clinically appropriate patients under sterile conditions were incubated in a BacTAlert 3D Biomerieux, France culture device. Bacteria isolated from 164 specimens with growth detected, were identified by matrix-mediated laser desorptionionization-time-of-flight mass spectrometry MALDI-TOF MS based VITEK MS Biomerieux, France. Antibiotic susceptibility tests were performed on the VITEK 2 Compact Biomerieux, France device. Results: Staphylococcus aureus S. aureus 29.3 and coagulase negative Staphylococci CNS 29.3 were the most commonly grown microorganisms. Other microorganisms grown were Streptococcus spp. 9.1, Enterococcus spp. 6.1, Pseudomonas aeruginosa P. aeuruginosa 7.3, Escherichiae coli E. coli 4.3 and Klebsiella pneumoniae K. pneumoniae 4.3. When antibiotic susceptibility results were evaluated according to EUCAST restricted reporting criteria, linezolid in Gram-positive strains, amikacin in Enterobacterales, colistin and tigecycline in nonfermentative Gram-negative bacteria were found to be the most susceptible antibiotics. Conclusion: The continuous change in antibiotic susceptibility profiles in joint infections, the long duration of treatment and follow-up, and the increase in polymicrobial infections require regular monitoring of culture and antibiotic susceptibility tests. In our study, the distribution of microorganisms isolated from joint fluid samples of our hospital and the determination of antibiotic resistance profiles will contribute to the clinician in terms of guiding empirical treatment.

 Aims: Septic arthritis is joint inflammation. It is an orthopedic emergency that requires prompt diagnosis and treatment. In this study, it was aimed to examine the distribution and antibiotic resistance profiles of microorganisms isolated from joint fluid samples taken from patients who applied to the orthopedic clinic of our hospital in the last five years. Methods: In our study, 1162 joint fluid samples were sent to the medical microbiology laboratory of our hospital from the orthopedics and traumatology clinic between January 01, 2018 and December 31, 2022. Joint fluid samples taken from clinically appropriate patients under sterile conditions were incubated in a BacTAlert 3D Biomerieux, France culture device. Bacteria isolated from 164 specimens with growth detected, were identified by matrix-mediated laser desorptionionization-time-of-flight mass spectrometry MALDI-TOF MS based VITEK MS Biomerieux, France. Antibiotic susceptibility tests were performed on the VITEK 2 Compact Biomerieux, France device. Results: Staphylococcus aureus S. aureus 29.3 and coagulase negative Staphylococci CNS 29.3 were the most commonly grown microorganisms. Other microorganisms grown were Streptococcus spp. 9.1, Enterococcus spp. 6.1, Pseudomonas aeruginosa P. aeuruginosa 7.3, Escherichiae coli E. coli 4.3 and Klebsiella pneumoniae K. pneumoniae 4.3. When antibiotic susceptibility results were evaluated according to EUCAST restricted reporting criteria, linezolid in Gram-positive strains, amikacin in Enterobacterales, colistin and tigecycline in nonfermentative Gram-negative bacteria were found to be the most susceptible antibiotics. Conclusion: The continuous change in antibiotic susceptibility profiles in joint infections, the long duration of treatment and follow-up, and the increase in polymicrobial infections require regular monitoring of culture and antibiotic susceptibility tests. In our study, the distribution of microorganisms isolated from joint fluid samples of our hospital and the determination of antibiotic resistance profiles will contribute to the clinician in terms of guiding empirical treatment.

Makale detayları

Evaluation of distribution and susceptibility of microorganisms isolated from joint fluid cultures: five-year data

Yazarlar:

Halil Er | Özlem Koca | Nevgün Sepin Özen

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Tıbbi Bakteriyoloji

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.432-437

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302544

pISSN:

2718-0115

Joint fluid

antibiotic resistance rates

septic arthritis

Staphylococcus aureus

Sayı

Makale detayları

Evaluation of distribution and susceptibility of microorganisms isolated from joint fluid cultures: five-year data

Yazarlar: Halil Er | Özlem Koca | Nevgün Sepin Özen

Yayıncı: MediHealth Academy Yayıncılık

Ana Başlıklar: Tıbbi Bakteriyoloji

Dergi: Anatolian Current Medical Journal Vol.5 Issue 4, pp.432-437

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001302544

pISSN: 2718-0115

Joint fluid

antibiotic resistance rates

septic arthritis

Staphylococcus aureus

Sayı

Yazarlar:

Halil Er | Özlem Koca | Nevgün Sepin Özen

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Tıbbi Bakteriyoloji

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.432-437

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302544

pISSN:

2718-0115