Effects of different calcium concentrations on in vitro shoot and root development in Gök Üzüm (Vitis vinifera L.) cultivar

Kevser Yazar

Makale detayları

Effects of different calcium concentrations on in vitro shoot and root development in Gök Üzüm (Vitis vinifera L.) cultivar

Yazarlar:

Kevser Yazar

Yayıncı:

Harran University

Ana Başlıklar:

Şarapçılık ve Bağcılık | Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Dergi:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.29 Issue 3, pp.577-589

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358653

pISSN:

2587-1358

Anahtar kelimeler:

Özet:

 In this study, the effects of different calcium CaCl₂2H₂O concentrations on shoot development, shoot tip necrosis STN, and rooting were investigated in the Gök Üzüm Vitis vinifera L. cultivar under in vitro conditions. During the first subculture, although shoot formation was promoted in the 1 mg L¹ BAP treatment, the STN rate was found to be high. Culture media containing 120, 180 and 240.0 mg L¹ CaCl₂2H₂O provided significant increases in parameters such as shoot length, number of nodes and leaf area compared to MS and BAP media. In addition, STN formation was completely eliminated at 180 and 240.0 mg L¹ calcium levels. The hormone-free MS0 medium gave the lowest values in all parameters. The suppression of STN was associated with the stabilizing effect of Ca² ions on the cell wall and the maintenance of plasma membrane integrity. In addition, Ca² plays an important role as a signaling element in plant physiology and influences hormonal regulations, particularly those related to auxin transport. During the rooting the highest root fresh weight was obtained with 240 mg L¹ CaCl₂2H₂O, indicating the contribution of calcium to root tissue development and integrity. The findings revealed that calcium enhances plantlet quality both morphologically and physiologically. Consequently, culture media enriched with 180240 mg L¹ CaCl₂2H₂O have the potential to increase the efficiency of grapevine micropropagation by promoting shoot development, reducing STN, and supporting root formation. Further studies are recommended to comprehensively evaluate the roles of different calcium sources and application strategies in plant tissue culture.

 In this study, the effects of different calcium CaCl₂2H₂O concentrations on shoot development, shoot tip necrosis STN, and rooting were investigated in the Gök Üzüm Vitis vinifera L. cultivar under in vitro conditions. During the first subculture, although shoot formation was promoted in the 1 mg L¹ BAP treatment, the STN rate was found to be high. Culture media containing 120, 180 and 240.0 mg L¹ CaCl₂2H₂O provided significant increases in parameters such as shoot length, number of nodes and leaf area compared to MS and BAP media. In addition, STN formation was completely eliminated at 180 and 240.0 mg L¹ calcium levels. The hormone-free MS0 medium gave the lowest values in all parameters. The suppression of STN was associated with the stabilizing effect of Ca² ions on the cell wall and the maintenance of plasma membrane integrity. In addition, Ca² plays an important role as a signaling element in plant physiology and influences hormonal regulations, particularly those related to auxin transport. During the rooting the highest root fresh weight was obtained with 240 mg L¹ CaCl₂2H₂O, indicating the contribution of calcium to root tissue development and integrity. The findings revealed that calcium enhances plantlet quality both morphologically and physiologically. Consequently, culture media enriched with 180240 mg L¹ CaCl₂2H₂O have the potential to increase the efficiency of grapevine micropropagation by promoting shoot development, reducing STN, and supporting root formation. Further studies are recommended to comprehensively evaluate the roles of different calcium sources and application strategies in plant tissue culture.

 In this study, the effects of different calcium CaCl₂2H₂O concentrations on shoot development, shoot tip necrosis STN, and rooting were investigated in the Gök Üzüm Vitis vinifera L. cultivar under in vitro conditions. During the first subculture, although shoot formation was promoted in the 1 mg L¹ BAP treatment, the STN rate was found to be high. Culture media containing 120, 180 and 240.0 mg L¹ CaCl₂2H₂O provided significant increases in parameters such as shoot length, number of nodes and leaf area compared to MS and BAP media. In addition, STN formation was completely eliminated at 180 and 240.0 mg L¹ calcium levels. The hormone-free MS0 medium gave the lowest values in all parameters. The suppression of STN was associated with the stabilizing effect of Ca² ions on the cell wall and the maintenance of plasma membrane integrity. In addition, Ca² plays an important role as a signaling element in plant physiology and influences hormonal regulations, particularly those related to auxin transport. During the rooting the highest root fresh weight was obtained with 240 mg L¹ CaCl₂2H₂O, indicating the contribution of calcium to root tissue development and integrity. The findings revealed that calcium enhances plantlet quality both morphologically and physiologically. Consequently, culture media enriched with 180240 mg L¹ CaCl₂2H₂O have the potential to increase the efficiency of grapevine micropropagation by promoting shoot development, reducing STN, and supporting root formation. Further studies are recommended to comprehensively evaluate the roles of different calcium sources and application strategies in plant tissue culture.

 Bu çalışma kapsamında, Gök Üzüm Vitis vinifera L. çeşidinde in vitro koşullarda farklı kalsiyum CaCl₂2H₂O konsantrasyonlarının sürgün gelişimi, sürgün ucu nekrozu STN ve köklenme üzerine etkileri araştırılmıştır. Birinci altkültür aşamasında, 1 mg L¹ BAP uygulamasında sürgün oluşumunun teşvik edilmesine rağmen STN oranı yüksek bulunmuştur. 120, 180 ve 240.0 mg L¹ CaCl₂2H₂O içeren kültür ortamları ise; sürgün uzunluğu, boğum sayısı ve yaprak alanı gibi parametrelerde MS ve BAP ortamlarına göre anlamlı artış sağlamıştır. Ayrıca, 180 ve 240.0 mg L¹ kalsiyum düzeylerinde STN oluşumu tamamen ortadan kalkmıştır. Hormon içermeyen MS0 ortamı ise tüm parametrelerde en düşük değerleri vermiştir. STNnin baskılanması, Ca² iyonunun hücre duvarı stabilitesi ile plazma membranı bütünlüğünü koruyucu etkisiyle ilişkilendirilmiştir. Bunun yanında, Ca² bitki fizyolojisinde önemli bir sinyal iletim elemanı olarak görev yapmakta ve özellikle oksin taşınımıyla ilişkili hormonal düzenlemeleri etkilemektedir. Köklenme aşamasında ise 240 mg L¹ CaCl₂2H₂O dozunda en yüksek kök ağırlığı elde edilmiş; bu da kalsiyumun kök dokularının gelişimi ve bütünlüğüne katkı sağladığını göstermiştir. Elde edilen bulgular, kalsiyumun hem morfolojik hem de fizyolojik açıdan bitkicik kalitesini artırdığını ortaya koymaktadır. Sonuç olarak, 180240 mg L¹ CaCl₂2H₂O içeren kültür ortamlarının sürgün gelişimini teşvik ederek, STNyi azaltarak ve kök gelişimini destekleyerek asma mikroçoğaltımında verimliliği artırma potansiyeline sahip olduğu kaydedilmiştir. Gelecek çalışmalarda, farklı kalsiyum kaynaklarının ve uygulama stratejilerinin bitki doku kültürü süreçlerindeki rolünün daha kapsamlı biçimde değerlendirilmesi önerilmektedir.

Makale detayları

Effects of different calcium concentrations on in vitro shoot and root development in Gök Üzüm (Vitis vinifera L.) cultivar

Yazarlar:

Kevser Yazar

Yayıncı:

Harran University

Ana Başlıklar:

Şarapçılık ve Bağcılık | Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Dergi:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.29 Issue 3, pp.577-589

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358653

pISSN:

2587-1358

Micropropagation

calcium

shoot tip necrosis

shoot development

plantlet qualityMikroçoğaltım

kalsiyum

sürgün ucu nekrozu

sürgün gelişimi

bitkicik kalitesi

Sayı

Makale detayları

Yazarlar: Kevser Yazar

Yayıncı: Harran University

Ana Başlıklar: Şarapçılık ve Bağcılık | Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Dergi: Harran Tarım ve Gıda Bilimleri Dergisi Vol.29 Issue 3, pp.577-589

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001358653

pISSN: 2587-1358

Sayı

Yazarlar:

Kevser Yazar

Yayıncı:

Harran University

Ana Başlıklar:

Şarapçılık ve Bağcılık | Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Dergi:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.29 Issue 3, pp.577-589

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358653

pISSN:

2587-1358