Goat Sperm Vitrification

Çiğdem Çebi; Ricardo Faundez

Makale detayları

Goat Sperm Vitrification

Yazarlar:

Çiğdem Çebi | Ricardo Faundez

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 4, pp.521-529

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001300382

pISSN:

1308-1594

Anahtar kelimeler:

Özet:

 The vitrification method is a freezing method in which the cooling rate is very high and high concentration permeable cryoprotectants are used. The vitrification method, which has a wide application area in embryo freezing, was applied to the storage of goat semen and the effect of the vitrification method on semen quality was determined. For this purpose, semen samples taken from 4 healthy goats via artificial vagina were divided into 4 equal groups. While the semen samples in Group 1 were frozen with the conventional method with Tris-sitrate modified solution containing 20 egg yolk and 5 glycerol, the remaining sperm samples were frozen with 0.1 M Group II, 0.25 M Group III and 0.5 M Group IV was diluted with Human Tubal Fluid diluent containing sucrose and vitrification method was applied. Standard semen analysis spermatozoa motility, plasma membrane integrity, sperm morphology, chromatin integrity was performed on all semen samples after conventional freezing and vitrification. As a result, attempts to vitrify goat spermatozoa using non-permeable cryoprotectants have resulted in very low viability. The physical and chemical changes occurring during vitrification negatively affected the motility, viability and sperm morphology of spermatozoa.

 The vitrification method is a freezing method in which the cooling rate is very high and high concentration permeable cryoprotectants are used. The vitrification method, which has a wide application area in embryo freezing, was applied to the storage of goat semen and the effect of the vitrification method on semen quality was determined. For this purpose, semen samples taken from 4 healthy goats via artificial vagina were divided into 4 equal groups. While the semen samples in Group 1 were frozen with the conventional method with Tris-sitrate modified solution containing 20 egg yolk and 5 glycerol, the remaining sperm samples were frozen with 0.1 M Group II, 0.25 M Group III and 0.5 M Group IV was diluted with Human Tubal Fluid diluent containing sucrose and vitrification method was applied. Standard semen analysis spermatozoa motility, plasma membrane integrity, sperm morphology, chromatin integrity was performed on all semen samples after conventional freezing and vitrification. As a result, attempts to vitrify goat spermatozoa using non-permeable cryoprotectants have resulted in very low viability. The physical and chemical changes occurring during vitrification negatively affected the motility, viability and sperm morphology of spermatozoa.

 The vitrification method is a freezing method in which the cooling rate is very high and high concentration permeable cryoprotectants are used. The vitrification method, which has a wide application area in embryo freezing, was applied to the storage of goat semen and the effect of the vitrification method on semen quality was determined. For this purpose, semen samples taken from 4 healthy goats via artificial vagina were divided into 4 equal groups. While the semen samples in Group 1 were frozen with the conventional method with Tris-sitrate modified solution containing 20 egg yolk and 5 glycerol, the remaining sperm samples were frozen with 0.1 M Group II, 0.25 M Group III and 0.5 M Group IV was diluted with Human Tubal Fluid diluent containing sucrose and vitrification method was applied. Standard semen analysis spermatozoa motility, plasma membrane integrity, sperm morphology, chromatin integrity was performed on all semen samples after conventional freezing and vitrification. As a result, attempts to vitrify goat spermatozoa using non-permeable cryoprotectants have resulted in very low viability. The physical and chemical changes occurring during vitrification negatively affected the motility, viability and sperm morphology of spermatozoa.

 Vitrifikasyon metodu, soğutma hızının çok yüksek olduğu, yüksek konsatrasyonlu permeable kriyopektanların kullanıldığı bir dondurma yöntemidir. Embriyo dondurulmasında oldukça geniş uygulama alanı bulan vitrifikasyon methodunun teke spermasının saklanmasında uygulanarak sperma kalitesi üzerine vitrifikasyon methodunun etkisi belirlenmiştir. Bu amaçla 4 sağlıklı tekeden suni vajen yoluyla alınan sperma örnekleri 4 eşit gruba ayrılmıştır. Grup 1deki sperma örnekleri 20 yumurta sarısı ve 5 gliserol içeren Tris-Sitrat modifiye solüsyonu ile konvansiyonel methodla dondurulurken, geri kalan sperm örnekleri ise 0.1 M Grup 2, 0.25 M Grup 3 ve 0.5 M Grup 4 sükroz içeren Human Tubal Fluid sulandırıcısı ile sulandırılıp vitrifikasyon işlemine tabi tutulmuştur. Tüm sperma örneklerinde konvansiyonel dondurma ve vitrifikasyon işlemi sonrası standart sperma analizi spermatozoa motilitesi, plasma membran bütünlüğü, sperm morfolojisi, kromatin bütünlüğü yapılmıştır. Sonuç olarak, non-permeable kriyoprotektanlar kullanılarak teke spermatozoasını vitrifiye etmek için girişilen deneyimler çok düşük canlılıkla sonuçlanmıştır. Vitrifikasyon sırasında olusan fiziksel ve kimyasal değişiklikler, spermatozoanın motilitesini, canlılıgını, sperm morfolojisini olumsuz şekilde etkilemiştir.

Makale detayları

Goat Sperm Vitrification

Yazarlar:

Çiğdem Çebi | Ricardo Faundez

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 4, pp.521-529

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001300382

pISSN:

1308-1594

Goat

Semen

Sucrose

VitrificationSperma

Sükroz

Teke

Vitrifikasyon

Sayı

Makale detayları

Yazarlar: Çiğdem Çebi | Ricardo Faundez

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Veteriner Bilimleri (Diğer)

Dergi: Kocatepe Veteriner Dergisi Vol.16 Issue 4, pp.521-529

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001300382

pISSN: 1308-1594

Sayı

Yazarlar:

Çiğdem Çebi | Ricardo Faundez

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 4, pp.521-529

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001300382

pISSN:

1308-1594