Ovicidal Effect of Beauveria bassiana Vuillemin Isolates on Tetranychus urticae Koch (Acari: Tetranychidae)

Asiye Uzun Yiğit

Makale detayları

Ovicidal Effect of Beauveria bassiana Vuillemin Isolates on Tetranychus urticae Koch (Acari: Tetranychidae)

Yazarlar:

Asiye Uzun Yiğit

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Entomoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.24 Issue 3, pp.485-491

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001317586

pISSN:

2149-3367

Anahtar kelimeler:

Özet:

 This study aimed to determine the ovicidal effect of three different doses 1x106 sporeml, 1x107 sporeml and 1x108 sporeml of Beauveria bassiana isolates BY2 Burdur, Yeşilova, BIM-001 Isparta, Center and IGÇ Isparta, Center on Tetranychus urticae under laboratory conditions. The commercial preparation Nostalgist Agrobest Company, Beauveria bassiana strain Bb-1 and water containing Tween 80 0,1 were used as control. Experiments were carried out in plastic petri dishes 9 cm with 5 replications. Bean leaf disks 4 cm were placed on sterile water-saturated cotton held in a Petri plate. Then, 10 adult individuals were transferred to leaf discs and allowed to lay eggs. Each petri plate was set to contain 20 eggsleaf discs. The spore suspension of each entomopathogenic fungus isolate was applied to the leaf discs containing eggs for 10 seconds with the help of a modified device that provides spraying at 4 bar pressure. Observations started 24 hours after the application and egg deaths were recorded. As a result, 7 days after application of 1x108 sporeml concentrations of B. bassiana BIM-001, BY2, and IGÇ isolates, the mortality rates in T. urticae eggs were determined as 71, 66, and 59, respectively. Application of B. bassiana strain Bb-1 was found to be statistically significant compared to applications of other entomopathogenic fungus isolates. However, it was determined that there was no difference between the mortality rates in B. bassiana BIM-001 1x108, 1x107 sporeml and B. bassiana BY2 1x108 sporeml applications p0.005.

 This study aimed to determine the ovicidal effect of three different doses 1x106 sporeml, 1x107 sporeml and 1x108 sporeml of Beauveria bassiana isolates BY2 Burdur, Yeşilova, BIM-001 Isparta, Center and IGÇ Isparta, Center on Tetranychus urticae under laboratory conditions. The commercial preparation Nostalgist Agrobest Company, Beauveria bassiana strain Bb-1 and water containing Tween 80 0,1 were used as control. Experiments were carried out in plastic petri dishes 9 cm with 5 replications. Bean leaf disks 4 cm were placed on sterile water-saturated cotton held in a Petri plate. Then, 10 adult individuals were transferred to leaf discs and allowed to lay eggs. Each petri plate was set to contain 20 eggsleaf discs. The spore suspension of each entomopathogenic fungus isolate was applied to the leaf discs containing eggs for 10 seconds with the help of a modified device that provides spraying at 4 bar pressure. Observations started 24 hours after the application and egg deaths were recorded. As a result, 7 days after application of 1x108 sporeml concentrations of B. bassiana BIM-001, BY2, and IGÇ isolates, the mortality rates in T. urticae eggs were determined as 71, 66, and 59, respectively. Application of B. bassiana strain Bb-1 was found to be statistically significant compared to applications of other entomopathogenic fungus isolates. However, it was determined that there was no difference between the mortality rates in B. bassiana BIM-001 1x108, 1x107 sporeml and B. bassiana BY2 1x108 sporeml applications p0.005.

 This study aimed to determine the ovicidal effect of three different doses 1x106 sporeml, 1x107 sporeml and 1x108 sporeml of Beauveria bassiana isolates BY2 Burdur, Yeşilova, BIM-001 Isparta, Center and IGÇ Isparta, Center on Tetranychus urticae under laboratory conditions. The commercial preparation Nostalgist Agrobest Company, Beauveria bassiana strain Bb-1 and water containing Tween 80 0,1 were used as control. Experiments were carried out in plastic petri dishes 9 cm with 5 replications. Bean leaf disks 4 cm were placed on sterile water-saturated cotton held in a Petri plate. Then, 10 adult individuals were transferred to leaf discs and allowed to lay eggs. Each petri plate was set to contain 20 eggsleaf discs. The spore suspension of each entomopathogenic fungus isolate was applied to the leaf discs containing eggs for 10 seconds with the help of a modified device that provides spraying at 4 bar pressure. Observations started 24 hours after the application and egg deaths were recorded. As a result, 7 days after application of 1x108 sporeml concentrations of B. bassiana BIM-001, BY2, and IGÇ isolates, the mortality rates in T. urticae eggs were determined as 71, 66, and 59, respectively. Application of B. bassiana strain Bb-1 was found to be statistically significant compared to applications of other entomopathogenic fungus isolates. However, it was determined that there was no difference between the mortality rates in B. bassiana BIM-001 1x108, 1x107 sporeml and B. bassiana BY2 1x108 sporeml applications p0.005.

 Bu çalışma ile Beauveria bassiananın BY2 Burdur, Yeşilova, BIM-001 Isparta, Merkez ve IGÇ Isparta, Merkez izolatlarının üç farklı dozunun 1x106 sporml, 1x107 sporml ve 1x108 sporml Tetranychus urticae üzerinde ovisidal etkisinin laboratuvar koşullarında belirlenmesi hedeflenmiştir. Ticari preparat Nostalgist Agrobest Grup Ltd. Şti, Beauveria bassiana Bb-1 ırkı ve kontrol olarak Tween 80  0,1 içeren saf su kullanılmıştır. Denemeler plastik petrilerde 9 cm 5 tekerrürlü olarak yürütülmüştür. Fasulye yaprak diskleri 4 cm petri plakasında tutulan steril suya doymuş pamuk üzerinde yerleştirilmiştir. Daha sonra 10 ergin birey, yaprak disklere aktarılarak yumurta bırakmalarına izin verilmiştir. Her bir petri plakasında 20 yumurtayaprak diski olacak şekilde ayarlanmıştır. Her bir entomopatojen fungus izolatına ait spor süspansiyonu 4 bar basınçta püskürtme sağlayan modifiye edilmiş bir düzenek yardımıyla 10 sn süre ile yumurta içeren yaprak disklerin üzerine uygulanmıştır. Gözlemlere uygulamadan 24 saat sonra başlanarak yumurta ölümleri kaydedilmiştir. Sonuç olarak, B. bassiana BIM-001, BY2 ve IGÇ izolatlarının 1x108 sporml konsantrasyonları uygulandıktan 7 gün sonra T. urticae yumurtalarında meydana gelen ölüm oranları sırasıyla 71, 66 ve 59 olarak belirlenmiştir. Beauveria bassiana strain Bb-1 uygulaması diğer entomopatojen fungus izolatlarına ait uygulamalardan istatistiksel olarak anlamlı bulunmuştur. Bununla birlikte B. bassiana BIM-001 1x108, 1x107 sporml ve B. bassiana BY2 1x108 sporml uygulamalarındaki ölüm oranları arasında fark olmadığı saptanmıştır p0.005.

Makale detayları

Ovicidal Effect of Beauveria bassiana Vuillemin Isolates on Tetranychus urticae Koch (Acari: Tetranychidae)

Yazarlar:

Asiye Uzun Yiğit

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Entomoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.24 Issue 3, pp.485-491

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001317586

pISSN:

2149-3367

Microbial control

Entomopathogenic fungus

Spore suspension

Two spotted spider miteMikrobiyal mücadele

Entomopatojen fungus

Spor süspansiyonu

İki noktalı kırmızıörümcek

Sayı

Makale detayları

Yazarlar: Asiye Uzun Yiğit

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Entomoloji

Dergi: Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.24 Issue 3, pp.485-491

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001317586

pISSN: 2149-3367

Sayı

Yazarlar:

Asiye Uzun Yiğit

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Entomoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.24 Issue 3, pp.485-491

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001317586

pISSN:

2149-3367