Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross

Mehtap Şahin ÇEVİK; Gloria A. MOORE

Makale detayları

Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross

Yazarlar:

Mehtap Şahin ÇEVİK | Gloria A. MOORE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2007 Vol.31 Issue 2, pp.79-86

Belge Tipi:

Makale

Yayın Tarihi:

1/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000723783

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata (L.) Raf.) x C. sinensis (L.) Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers. A total of 111 random amplified polymorphic DNA (RAPD) markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.

 

This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata (L.) Raf.) x C. sinensis (L.) Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers. A total of 111 random amplified polymorphic DNA (RAPD) markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.

Makale detayları

Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross

Yazarlar: Mehtap Şahin ÇEVİK | Gloria A. MOORE

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2007 Vol.31 Issue 2, pp.79-86

Belge Tipi: Makale

Yayın Tarihi: 1/2007

Belge Dili: Türkçe

Document ID (Org No.): 2000723783

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Mehtap Şahin ÇEVİK | Gloria A. MOORE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2007 Vol.31 Issue 2, pp.79-86

Belge Tipi:

Makale

Yayın Tarihi:

1/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000723783

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X