Anesthesia/stereotaxic surgery exposure affects cognitive impairment and hippocampal synaptic plasticity dysfunction in male rats

Osman Sinen; Ayşegül Gemici Sinen; Narin Derin

Makale detayları

Anesthesia/stereotaxic surgery exposure affects cognitive impairment and hippocampal synaptic plasticity dysfunction in male rats

Yazarlar:

Osman Sinen | Ayşegül Gemici Sinen | Narin Derin

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Central Nervous System

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 2, pp.259-263

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317659

pISSN:

1309-4483

Anahtar kelimeler:

Özet:

 Postoperative cognitive dysfunction POCD is a frequent complication after major surgery and anesthesia exposure, particularly in advanced aging. However, the effects of anesthesiastereotaxic surgery ASS on cognitive function in rats remain inadequately understood. This study aims to elucidate the effects of ASS on cognitive function in male Sprague Dawley rats and explore potential alterations in hippocampal synaptic plasticity. Male Sprague Dawley rats, aged 6 months, were subjected to anesthesia A or ASS, involving anesthesia with ketaminexylazine 5010 mgkg, ip and stereotaxic surgery to create a burr hole above the lateral ventricle. Anxiety-like behavior and locomotor activity were assessed using the open-field test one week post-surgery. Spatial memory and learning were evaluated through the Y-Maze test and novel object recognition test NORT. Moreover, to determine hippocampal synaptic plasticity, we evaluated hippocampal long-term potentiation LTP at perforant pathway-dentate gyrus synapses. No statistically significant differences in anxiety and motor activity were observed between groups. Compared to the no-treatment group, the ASS group rats exhibited a significantly p0.05 lower exploration of a novel arm and novel object in the Y-Maze and NORT, respectively. Furthermore, on postoperative day 7, LTP induced by high-frequency stimulation in the dentate gyrus region was attenuated p0.05 in the ASS group compared to the no-treatment group. These findings indicated that ASS induced cognitive decline and functional synaptic plasticity dysfunction. The results of the present study may be useful for future studies examining cognitive functions in experimental models involving such procedures.

 Postoperative cognitive dysfunction POCD is a frequent complication after major surgery and anesthesia exposure, particularly in advanced aging. However, the effects of anesthesiastereotaxic surgery ASS on cognitive function in rats remain inadequately understood. This study aims to elucidate the effects of ASS on cognitive function in male Sprague Dawley rats and explore potential alterations in hippocampal synaptic plasticity. Male Sprague Dawley rats, aged 6 months, were subjected to anesthesia A or ASS, involving anesthesia with ketaminexylazine 5010 mgkg, ip and stereotaxic surgery to create a burr hole above the lateral ventricle. Anxiety-like behavior and locomotor activity were assessed using the open-field test one week post-surgery. Spatial memory and learning were evaluated through the Y-Maze test and novel object recognition test NORT. Moreover, to determine hippocampal synaptic plasticity, we evaluated hippocampal long-term potentiation LTP at perforant pathway-dentate gyrus synapses. No statistically significant differences in anxiety and motor activity were observed between groups. Compared to the no-treatment group, the ASS group rats exhibited a significantly p0.05 lower exploration of a novel arm and novel object in the Y-Maze and NORT, respectively. Furthermore, on postoperative day 7, LTP induced by high-frequency stimulation in the dentate gyrus region was attenuated p0.05 in the ASS group compared to the no-treatment group. These findings indicated that ASS induced cognitive decline and functional synaptic plasticity dysfunction. The results of the present study may be useful for future studies examining cognitive functions in experimental models involving such procedures.

 Postoperative cognitive dysfunction POCD is a frequent complication after major surgery and anesthesia exposure, particularly in advanced aging. However, the effects of anesthesiastereotaxic surgery ASS on cognitive function in rats remain inadequately understood. This study aims to elucidate the effects of ASS on cognitive function in male Sprague Dawley rats and explore potential alterations in hippocampal synaptic plasticity. Male Sprague Dawley rats, aged 6 months, were subjected to anesthesia A or ASS, involving anesthesia with ketaminexylazine 5010 mgkg, ip and stereotaxic surgery to create a burr hole above the lateral ventricle. Anxiety-like behavior and locomotor activity were assessed using the open-field test one week post-surgery. Spatial memory and learning were evaluated through the Y-Maze test and novel object recognition test NORT. Moreover, to determine hippocampal synaptic plasticity, we evaluated hippocampal long-term potentiation LTP at perforant pathway-dentate gyrus synapses. No statistically significant differences in anxiety and motor activity were observed between groups. Compared to the no-treatment group, the ASS group rats exhibited a significantly p0.05 lower exploration of a novel arm and novel object in the Y-Maze and NORT, respectively. Furthermore, on postoperative day 7, LTP induced by high-frequency stimulation in the dentate gyrus region was attenuated p0.05 in the ASS group compared to the no-treatment group. These findings indicated that ASS induced cognitive decline and functional synaptic plasticity dysfunction. The results of the present study may be useful for future studies examining cognitive functions in experimental models involving such procedures.

 Postoperative cognitive dysfunction POCD is a frequent complication after major surgery and anesthesia exposure, particularly in advanced aging. However, the effects of anesthesiastereotaxic surgery ASS on cognitive function in rats remain inadequately understood. This study aims to elucidate the effects of ASS on cognitive function in male Sprague Dawley rats and explore potential alterations in hippocampal synaptic plasticity. Male Sprague Dawley rats, aged 6 months, were subjected to anesthesia A or ASS, involving anesthesia with ketaminexylazine 5010 mgkg, ip and stereotaxic surgery to create a burr hole above the lateral ventricle. Anxiety-like behavior and locomotor activity were assessed using the open-field test one week post-surgery. Spatial memory and learning were evaluated through the Y-Maze test and novel object recognition test NORT. Moreover, to determine hippocampal synaptic plasticity, we evaluated hippocampal long-term potentiation LTP at perforant pathway-dentate gyrus synapses. No statistically significant differences in anxiety and motor activity were observed between groups. Compared to the no-treatment group, the ASS group rats exhibited a significantly p0.05 lower exploration of a novel arm and novel object in the Y-Maze and NORT, respectively. Furthermore, on postoperative day 7, LTP induced by high-frequency stimulation in the dentate gyrus region was attenuated p0.05 in the ASS group compared to the no-treatment group. These findings indicated that ASS induced cognitive decline and functional synaptic plasticity dysfunction. The results of the present study may be useful for future studies examining cognitive functions in experimental models involving such procedures.

Makale detayları

Anesthesia/stereotaxic surgery exposure affects cognitive impairment and hippocampal synaptic plasticity dysfunction in male rats

Yazarlar:

Osman Sinen | Ayşegül Gemici Sinen | Narin Derin

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Central Nervous System

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 2, pp.259-263

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317659

pISSN:

1309-4483

Stereotaxic surgery

Anesthesia

Postoperative cognitive dysfunction

Sayı

Makale detayları

Anesthesia/stereotaxic surgery exposure affects cognitive impairment and hippocampal synaptic plasticity dysfunction in male rats

Yazarlar: Osman Sinen | Ayşegül Gemici Sinen | Narin Derin

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Central Nervous System

Dergi: Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 2, pp.259-263

Belge Tipi: Makale

Yayın Tarihi: 5/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001317659

pISSN: 1309-4483

Stereotaxic surgery

Anesthesia

Postoperative cognitive dysfunction

Sayı

Yazarlar:

Osman Sinen | Ayşegül Gemici Sinen | Narin Derin

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Central Nervous System

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 2, pp.259-263

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317659

pISSN:

1309-4483