Significance of CD49f in diagnosis of minimal residual disease in pediatric acute leukemia

Tuba Kurt; Ebru Yılmaz; Ekrem Ünal; Musa Karakükcü; Türkan Patiroğlu

Makale detayları

Significance of CD49f in diagnosis of minimal residual disease in pediatric acute leukemia

Yazarlar:

Tuba Kurt | Ebru Yılmaz | Ekrem Ünal | Musa Karakükcü | Türkan Patiroğlu

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Health Care Administration

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.40 Issue 3, pp.426-430

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300581

pISSN:

1309-4483

Anahtar kelimeler:

Özet:

 Minimal residual disease MRD is the most important prognostic indicator in acute lymphoblastic leukemia ALL in childhood. Multiparametric flow cytometry FCM is a technique that is often used to determine MRD, and many markers have been identified. Another marker examined in the MRD analysis is CD49f. We aimed to determine the importance of CD49f expression in MRD detection. Immunophenotyping MRD and CD49f expressions were performed in patients with Pre-B cell ALL at the diagnosis, and on the day 15. 27 patients were included FM: 1017. The mean age was 6.64.8 years. 6 22.2 patients were in the standard risk group, 14 51.9 patients were in the intermediate risk group, and 7 25.9 patients were in the high-risk group. MRD was detected in 15 55.6 patients. Cytomorphological remission was observed in 21 77.7 patients on the 15th day. 10 of these patients 66.6 were MRD positive. CD49f levels at diagnosis and at 15th day were mean 38.4  22.1 and 5.412.6, respectively. A significant decrease in CD49f expression was observed at follow up p0.00. Mean CD49f levels in MRD positive and MRD negative patients were 7.817 and 2.82 at day 15, respectively p0.64. There was no correlation between MRD and CD49f at day 15 p0.54. We observed that leukemic blasts express CD49f at a high rate, and this expression continues to decrease on the 15th day. We concluded that studies including more patients are required to assess the performance and importance of CD49f as an indicator in MRD.

 Minimal residual disease MRD is the most important prognostic indicator in acute lymphoblastic leukemia ALL in childhood. Multiparametric flow cytometry FCM is a technique that is often used to determine MRD, and many markers have been identified. Another marker examined in the MRD analysis is CD49f. We aimed to determine the importance of CD49f expression in MRD detection. Immunophenotyping MRD and CD49f expressions were performed in patients with Pre-B cell ALL at the diagnosis, and on the day 15. 27 patients were included FM: 1017. The mean age was 6.64.8 years. 6 22.2 patients were in the standard risk group, 14 51.9 patients were in the intermediate risk group, and 7 25.9 patients were in the high-risk group. MRD was detected in 15 55.6 patients. Cytomorphological remission was observed in 21 77.7 patients on the 15th day. 10 of these patients 66.6 were MRD positive. CD49f levels at diagnosis and at 15th day were mean 38.4  22.1 and 5.412.6, respectively. A significant decrease in CD49f expression was observed at follow up p0.00. Mean CD49f levels in MRD positive and MRD negative patients were 7.817 and 2.82 at day 15, respectively p0.64. There was no correlation between MRD and CD49f at day 15 p0.54. We observed that leukemic blasts express CD49f at a high rate, and this expression continues to decrease on the 15th day. We concluded that studies including more patients are required to assess the performance and importance of CD49f as an indicator in MRD.

 Minimal residual disease MRD is the most important prognostic indicator in acute lymphoblastic leukemia ALL in childhood. Multiparametric flow cytometry FCM is a technique that is often used to determine MRD, and many markers have been identified. Another marker examined in the MRD analysis is CD49f. We aimed to determine the importance of CD49f expression in MRD detection. Immunophenotyping MRD and CD49f expressions were performed in patients with Pre-B cell ALL at the diagnosis, and on the day 15. 27 patients were included FM: 1017. The mean age was 6.64.8 years. 6 22.2 patients were in the standard risk group, 14 51.9 patients were in the intermediate risk group, and 7 25.9 patients were in the high-risk group. MRD was detected in 15 55.6 patients. Cytomorphological remission was observed in 21 77.7 patients on the 15th day. 10 of these patients 66.6 were MRD positive. CD49f levels at diagnosis and at 15th day were mean 38.4  22.1 and 5.412.6, respectively. A significant decrease in CD49f expression was observed at follow up p0.00. Mean CD49f levels in MRD positive and MRD negative patients were 7.817 and 2.82 at day 15, respectively p0.64. There was no correlation between MRD and CD49f at day 15 p0.54. We observed that leukemic blasts express CD49f at a high rate, and this expression continues to decrease on the 15th day. We concluded that studies including more patients are required to assess the performance and importance of CD49f as an indicator in MRD.

 Minimal residual disease MRD is the most important prognostic indicator in acute lymphoblastic leukemia ALL in childhood. Multiparametric flow cytometry FCM is a technique that is often used to determine MRD, and many markers have been identified. Another marker examined in the MRD analysis is CD49f. We aimed to determine the importance of CD49f expression in MRD detection. Immunophenotyping MRD and CD49f expressions were performed in patients with Pre-B cell ALL at the diagnosis, and on the day 15. 27 patients were included FM: 1017. The mean age was 6.64.8 years. 6 22.2 patients were in the standard risk group, 14 51.9 patients were in the intermediate risk group, and 7 25.9 patients were in the high-risk group. MRD was detected in 15 55.6 patients. Cytomorphological remission was observed in 21 77.7 patients on the 15th day. 10 of these patients 66.6 were MRD positive. CD49f levels at diagnosis and at 15th day were mean 38.4  22.1 and 5.412.6, respectively. A significant decrease in CD49f expression was observed at follow up p0.00. Mean CD49f levels in MRD positive and MRD negative patients were 7.817 and 2.82 at day 15, respectively p0.64. There was no correlation between MRD and CD49f at day 15 p0.54. We observed that leukemic blasts express CD49f at a high rate, and this expression continues to decrease on the 15th day. We concluded that studies including more patients are required to assess the performance and importance of CD49f as an indicator in MRD.

Makale detayları

Significance of CD49f in diagnosis of minimal residual disease in pediatric acute leukemia

Yazarlar:

Tuba Kurt | Ebru Yılmaz | Ekrem Ünal | Musa Karakükcü | Türkan Patiroğlu

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Health Care Administration

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.40 Issue 3, pp.426-430

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300581

pISSN:

1309-4483

Acute lymphoblastic leukemia

minimal residual disease

CD49f

Acute lymphoblastic leukemia, minimal residual disease, CD49fAcute lymphoblastic leukemia

minimal residual disease

CD49f

Sayı

Makale detayları

Significance of CD49f in diagnosis of minimal residual disease in pediatric acute leukemia

Yazarlar: Tuba Kurt | Ebru Yılmaz | Ekrem Ünal | Musa Karakükcü | Türkan Patiroğlu

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Health Care Administration

Dergi: Deneysel ve Klinik Tıp Dergisi Vol.40 Issue 3, pp.426-430

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001300581

pISSN: 1309-4483

Acute lymphoblastic leukemia

minimal residual disease

CD49f

Acute lymphoblastic leukemia, minimal residual disease, CD49fAcute lymphoblastic leukemia

minimal residual disease

CD49f

Sayı

Yazarlar:

Tuba Kurt | Ebru Yılmaz | Ekrem Ünal | Musa Karakükcü | Türkan Patiroğlu

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Health Care Administration

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.40 Issue 3, pp.426-430

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300581

pISSN:

1309-4483