Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Sine ÖZMEN TOĞAY; Ayhan TEMİZ; Ayten ÇELEBİ; Leyla AÇIK; Sıddika Songül YALÇIN

Makale detayları

Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Yazarlar:

Sine ÖZMEN TOĞAY | Ayhan TEMİZ | Ayten ÇELEBİ | Leyla AÇIK | Sıddika Songül YALÇIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 3, pp.357-364

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812194

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and some virulence genes (agg2, gelE, efaAfm, ccf, cpd, cad, cylM, cylB, etc.) were investigated using polymerase chain reaction (PCR). All strains were sensitive to ampicillin, penicillin G, chloramphenicol, and vancomycin. None of the E. faecalis isolates contained vanA, vanB, or efaAfm genes. The results of this study indicated that there were no harmful enterococci strains in human milk and colostrum samples in terms of tested virulence factors and antibiotic resistance. Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.

 

Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and some virulence genes (agg2, gelE, efaAfm, ccf, cpd, cad, cylM, cylB, etc.) were investigated using polymerase chain reaction (PCR). All strains were sensitive to ampicillin, penicillin G, chloramphenicol, and vancomycin. None of the E. faecalis isolates contained vanA, vanB, or efaAfm genes. The results of this study indicated that there were no harmful enterococci strains in human milk and colostrum samples in terms of tested virulence factors and antibiotic resistance. Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.

Makale detayları

Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Yazarlar:

Sine ÖZMEN TOĞAY | Ayhan TEMİZ | Ayten ÇELEBİ | Leyla AÇIK | Sıddika Songül YALÇIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 3, pp.357-364

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812194

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Enterococcus faecalis

virulence gene

antibiotic resistance

human milk

colostrum

Sayı

Makale detayları

Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Yazarlar: Sine ÖZMEN TOĞAY | Ayhan TEMİZ | Ayten ÇELEBİ | Leyla AÇIK | Sıddika Songül YALÇIN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2014 Vol.38 Issue 3, pp.357-364

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000812194

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Enterococcus faecalis

virulence gene

antibiotic resistance

human milk

colostrum

Sayı

Yazarlar:

Sine ÖZMEN TOĞAY | Ayhan TEMİZ | Ayten ÇELEBİ | Leyla AÇIK | Sıddika Songül YALÇIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 3, pp.357-364

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812194

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152