Direct Detection of SARS CoV-2 by Practical Paper Based Imunoassay as an Instrument Free and Low Cost by CR3022

Dilek Çam Derin

Makale detayları

Direct Detection of SARS CoV-2 by Practical Paper Based Imunoassay as an Instrument Free and Low Cost by CR3022

Yazarlar:

Dilek Çam Derin

Yayıncı:

Erciyes University

Ana Başlıklar:

Sensör Teknolojisi | Biyomedikal Tanı | Nanoteknoloji (Diğer)

Dergi:

Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi Vol.40 Issue 3, pp.528-534

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001337254

pISSN:

1012-2354

Anahtar kelimeler:

Özet:

 Pandemia could be emerged at any time and resulted with death in the world. Severe acute respiratory syndrome coronavirus 2 SARS CoV-2 caused the latest pandemia. Because of the absence of rapid diagnostic assays pandemia has surged in and caused the death of lots of people as SARS CoV-2 appeared at first time. Although reverse transcriptase polymerase chain reaction RT-PCR accepted as the most reliable test was used for the viral detection at first time, there are shortcomings including the need of educated personnel and difficulty of the usage of this test in undeveloped countries. For this reason, developing the rapid assays for direct recognition of virus is always eesential. Paper based immunoassays are significant among these assays and they are known as dot-blot assay DBA. It is practical and easy to use since they are suitable for direct application of body fluids. Antibodies and aptamers are the most important reagents of diagnostic assays and it should be used having the high affinity of them. In this work, DBA was prepared by antibody-aptamer based sandwich assay and reported the suitibility of it for viral detection.

 Pandemia could be emerged at any time and resulted with death in the world. Severe acute respiratory syndrome coronavirus 2 SARS CoV-2 caused the latest pandemia. Because of the absence of rapid diagnostic assays pandemia has surged in and caused the death of lots of people as SARS CoV-2 appeared at first time. Although reverse transcriptase polymerase chain reaction RT-PCR accepted as the most reliable test was used for the viral detection at first time, there are shortcomings including the need of educated personnel and difficulty of the usage of this test in undeveloped countries. For this reason, developing the rapid assays for direct recognition of virus is always eesential. Paper based immunoassays are significant among these assays and they are known as dot-blot assay DBA. It is practical and easy to use since they are suitable for direct application of body fluids. Antibodies and aptamers are the most important reagents of diagnostic assays and it should be used having the high affinity of them. In this work, DBA was prepared by antibody-aptamer based sandwich assay and reported the suitibility of it for viral detection.

 Pandemia could be emerged at any time and resulted with death in the world. Severe acute respiratory syndrome coronavirus 2 SARS CoV-2 caused the latest pandemia. Because of the absence of rapid diagnostic assays pandemia has surged in and caused the death of lots of people as SARS CoV-2 appeared at first time. Although reverse transcriptase polymerase chain reaction RT-PCR accepted as the most reliable test was used for the viral detection at first time, there are shortcomings including the need of educated personnel and difficulty of the usage of this test in undeveloped countries. For this reason, developing the rapid assays for direct recognition of virus is always eesential. Paper based immunoassays are significant among these assays and they are known as dot-blot assay DBA. It is practical and easy to use since they are suitable for direct application of body fluids. Antibodies and aptamers are the most important reagents of diagnostic assays and it should be used having the high affinity of them. In this work, DBA was prepared by antibody-aptamer based sandwich assay and reported the suitibility of it for viral detection.

 Pandemi dünyada her an ortaya çıkabilir ve birçok ölümle sonuçlanabilir. En son görülen pandemiye şiddetli akut solunum yolu sendromu koronavirüs 2 SARS CoV-2 sebep olmuştur. SARS CoV-2 ilk kez ortaya çıktığı için hızlı tanı testlerinin yokluğu sebebiyle pandemi hızla ilerlemiş ve birçok kişinin ölümüne sebep olmuştur. Virüs tanısı için ilk olarak en güvenilir test olarak kabul edilen ters transkripsiyon polimeraz zincir reaksiyonu RT-PZR kullanılsa da eğitimli personel ihtiyacı duyması ve gelişmemiş ülkelerde bu testin uygulama zorluğu sebebiyle eksikleri vardır. Bu nedenle direkt virüs teşhisine yönelik hızlı testlerin geliştirilmesi her zaman elzemdir. Bu testler arasında kağıt tabanlı immünotestler önemlidir ve çoğunlukla dot-blot assay noktasal test-NT olarak bilinir. Vücut sıvılarının direkt uygulanmasına uygun olduğu için pratik ve kullanımı kolaydır. Antikor ve aptamerler tanı testlerinin en önemli bileşenleridir ve bunlardan afinitesi iyi olanlar kullanılmalıdır. Bu araştırmada antikor-aptamer sandviç testine dayalı olarak NT hazırlanmış ve viral teşhise uygun olup olmadığı bildirilmiştir.

Makale detayları

Direct Detection of SARS CoV-2 by Practical Paper Based Imunoassay as an Instrument Free and Low Cost by CR3022

Yazarlar:

Dilek Çam Derin

Yayıncı:

Erciyes University

Ana Başlıklar:

Sensör Teknolojisi | Biyomedikal Tanı | Nanoteknoloji (Diğer)

Dergi:

Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi Vol.40 Issue 3, pp.528-534

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001337254

pISSN:

1012-2354

Dot blot assay

viral diagnosis

paper assay

SARS CoV-2Dot blot assay

viral diagnosis

paper assay

SARS CoV-2

Sayı

Makale detayları

Yazarlar: Dilek Çam Derin

Yayıncı: Erciyes University

Ana Başlıklar: Sensör Teknolojisi | Biyomedikal Tanı | Nanoteknoloji (Diğer)

Dergi: Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi Vol.40 Issue 3, pp.528-534

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001337254

pISSN: 1012-2354

Sayı

Yazarlar:

Dilek Çam Derin

Yayıncı:

Erciyes University

Ana Başlıklar:

Sensör Teknolojisi | Biyomedikal Tanı | Nanoteknoloji (Diğer)

Dergi:

Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi Vol.40 Issue 3, pp.528-534

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001337254

pISSN:

1012-2354