Antioxidant and antimicrobial activities of the essential oil of Helichrysum chionophilum: an endemic plant of Türkiye

Tuba Acet; Kadriye Özcan

Makale detayları

Antioxidant and antimicrobial activities of the essential oil of Helichrysum chionophilum: an endemic plant of Türkiye

Yazarlar:

Tuba Acet | Kadriye Özcan

Yayıncı:

Giresun University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 2, pp.680-689

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291015

pISSN:

2564-7377

Anahtar kelimeler:

Özet:

 Diseases that develop due to microorganisms have become increasingly dangerous today. Especially in the fight against resistant microorganisms, there is a need for cheaper, natural and effective methods. Plant materials containing biologically active compounds come to the fore in this sense. Therefore, in the present study, the antioxidant and antimicrobial activities of the essential oil obtained from H. chionophilum collected from Gümüşhane were investigated. The essential oil was obtained by hydro-distillation using a Clevenger-type apparatus. Free radical scavenging activity was determined using the DPPH 2,2-diphenyl-1-picrylhydrazil method. Antimicrobial activity was examined using the microdilution method using 17 bacteria and 2 yeasts. According to the findings, IC50 value of the radical scavenging activity of the essential oil of H. chionophilum plant was found to be 260.00.2 µgmL. Also, the essential oil of the plant showed outstanding antimicrobial activity against Y. pseudotuberculosis MIC: 16 µgmL, S. epidermidis MIC: 16 µgmL, and C. albicans MIC: 16 µgmL and C. tropicalis MIC: 32 µgmL. The results indicate that H. chionophilum, which is an endemic species, and its essential oils can be used as a new and alternative natural agent thanks to its antioxidant and antimicrobial properties.

 Diseases that develop due to microorganisms have become increasingly dangerous today. Especially in the fight against resistant microorganisms, there is a need for cheaper, natural and effective methods. Plant materials containing biologically active compounds come to the fore in this sense. Therefore, in the present study, the antioxidant and antimicrobial activities of the essential oil obtained from H. chionophilum collected from Gümüşhane were investigated. The essential oil was obtained by hydro-distillation using a Clevenger-type apparatus. Free radical scavenging activity was determined using the DPPH 2,2-diphenyl-1-picrylhydrazil method. Antimicrobial activity was examined using the microdilution method using 17 bacteria and 2 yeasts. According to the findings, IC50 value of the radical scavenging activity of the essential oil of H. chionophilum plant was found to be 260.00.2 µgmL. Also, the essential oil of the plant showed outstanding antimicrobial activity against Y. pseudotuberculosis MIC: 16 µgmL, S. epidermidis MIC: 16 µgmL, and C. albicans MIC: 16 µgmL and C. tropicalis MIC: 32 µgmL. The results indicate that H. chionophilum, which is an endemic species, and its essential oils can be used as a new and alternative natural agent thanks to its antioxidant and antimicrobial properties.

 Diseases that develop due to microorganisms have become increasingly dangerous today. Especially in the fight against resistant microorganisms, there is a need for cheaper, natural and effective methods. Plant materials containing biologically active compounds come to the fore in this sense. Therefore, in the present study, the antioxidant and antimicrobial activities of the essential oil obtained from H. chionophilum collected from Gümüşhane were investigated. The essential oil was obtained by hydro-distillation using a Clevenger-type apparatus. Free radical scavenging activity was determined using the DPPH 2,2-diphenyl-1-picrylhydrazil method. Antimicrobial activity was examined using the microdilution method using 17 bacteria and 2 yeasts. According to the findings, IC50 value of the radical scavenging activity of the essential oil of H. chionophilum plant was found to be 260.00.2 µgmL. Also, the essential oil of the plant showed outstanding antimicrobial activity against Y. pseudotuberculosis MIC: 16 µgmL, S. epidermidis MIC: 16 µgmL, and C. albicans MIC: 16 µgmL and C. tropicalis MIC: 32 µgmL. The results indicate that H. chionophilum, which is an endemic species, and its essential oils can be used as a new and alternative natural agent thanks to its antioxidant and antimicrobial properties.

 Mikroorganizmalara bağlı olarak gelişen rahatsızlıklar günümüzde giderek tehlikeli bir hal almıştır. Özellikle, dirençli organizmalar ile mücadele noktasında, daha ucuz, doğal ve etkili yöntemlere ihtiyaç duyulmaktadır. Biyolojik olarak aktif bileşikleri barındıran bitki materyalleri bu manada ön plana çıkmaktadır. Bu nedenle, mevcut çalışmada, Gümüşhaneden toplanan Helichrysum chionophilumdan elde edilen uçucu yağın antioksidan ve antimikrobiyal aktiviteleri araştırılmıştır. Uçucu yağ, Clevenger tipi bir aparat kullanılarak hidro-damıtma yönteminden elde edildi. Serbest radikal yakalama aktivitesi, DPPH 2,2-difenil-1-pikrilhidrazil yöntemi kullanılarak belirlendi. Antimikrobiyal aktivite mikro dilüsyon yöntemi kullanılarak 17 bakteri ve 2 maya kullanılarak incelendi. Elde edilen bulgulara göre, H. chionophilum bitkisi radikal süpürme aktivitesinin IC50 değeri 260.00.2 µgmL olarak bulunmuştur. Ayrıca, bitkinin uçucu yağı, Y. pseudotuberculosis MIC: 16 µgmL, S. epidermidis MIC: 16 µgmL ve C. albicans MIC: 16 µgmL ve C. tropicalise MIC: 32 µgmL karşı olağanüstü antimikrobiyal aktivite göstermiştir. Elde edilen sonuçlar, endemik bir tür olan H. chionophilum ve uçucu yağlarının antioksidan ve antimikrobiyal özellikleri sayesinde yeni ve alternatif doğal bir ajan olarak kullanılabileceğini işaret etmektedir.

Makale detayları

Antioxidant and antimicrobial activities of the essential oil of Helichrysum chionophilum: an endemic plant of Türkiye

Yazarlar:

Tuba Acet | Kadriye Özcan

Yayıncı:

Giresun University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 2, pp.680-689

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291015

pISSN:

2564-7377

Antioxidant

antimicrobial

Helichrysum chionophilumAntioksidan

antimikrobiyal

Helichrysum chionophilum

Sayı

Makale detayları

Antioxidant and antimicrobial activities of the essential oil of Helichrysum chionophilum: an endemic plant of Türkiye

Yazarlar: Tuba Acet | Kadriye Özcan

Yayıncı: Giresun University

Ana Başlıklar: Yapısal Biyoloji

Dergi: Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 2, pp.680-689

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001291015

pISSN: 2564-7377

Antioxidant

antimicrobial

Helichrysum chionophilumAntioksidan

antimikrobiyal

Helichrysum chionophilum

Sayı

Yazarlar:

Tuba Acet | Kadriye Özcan

Yayıncı:

Giresun University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 2, pp.680-689

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291015

pISSN:

2564-7377