Purification of Glutathione Reductase Enzyme from Whiting Fish Gill Tissue and Investigation of Metal Inhibition

Seyit Batuhan Dudak; Kübra Işık; Betül Mitrovica; Ercan Soydan; Deniz Ekinci

Makale detayları

Purification of Glutathione Reductase Enzyme from Whiting Fish Gill Tissue and Investigation of Metal Inhibition

Yazarlar:

Seyit Batuhan Dudak | Kübra Işık | Betül Mitrovica | Ercan Soydan | Deniz Ekinci

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 6, pp.610-614

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001303237

pISSN:

2618-6578

Anahtar kelimeler:

Özet:

 Pollution in the seas basically accumulates in marine organisms. Heavy metal residues in aquatic ecosystems can pass through food and cause toxic effects and accumulations in human health. Glutathione reductase GR, which is among the basic enzymes, has an important place in the suppression of stress in the cell. In this study, glutathione reductase enzyme from whiting fish gill tissue was partially purified for the first time in the literature and the effects of heavy metal compounds on enzyme activity were determined. The purification process was carried out in three stages as homogenate preparation, ammonium sulfate precipitation and also dialysis. In conclusion the study, optimum level pH 7.0, optimal substrate concentration 2 mM NADPH and optimum buffer 150 mM KH2PO4 were determined. After partial purification, the inhibition effects of Cd2, Ni2, Zn2 as heavy metal ions were investigated. The IC50 levels of heavy metals were calculated as 20.17 µM, 33.7 µM and 59.31 µM, respectively.

 Pollution in the seas basically accumulates in marine organisms. Heavy metal residues in aquatic ecosystems can pass through food and cause toxic effects and accumulations in human health. Glutathione reductase GR, which is among the basic enzymes, has an important place in the suppression of stress in the cell. In this study, glutathione reductase enzyme from whiting fish gill tissue was partially purified for the first time in the literature and the effects of heavy metal compounds on enzyme activity were determined. The purification process was carried out in three stages as homogenate preparation, ammonium sulfate precipitation and also dialysis. In conclusion the study, optimum level pH 7.0, optimal substrate concentration 2 mM NADPH and optimum buffer 150 mM KH2PO4 were determined. After partial purification, the inhibition effects of Cd2, Ni2, Zn2 as heavy metal ions were investigated. The IC50 levels of heavy metals were calculated as 20.17 µM, 33.7 µM and 59.31 µM, respectively.

 Pollution in the seas basically accumulates in marine organisms. Heavy metal residues in aquatic ecosystems can pass through food and cause toxic effects and accumulations in human health. Glutathione reductase GR, which is among the basic enzymes, has an important place in the suppression of stress in the cell. In this study, glutathione reductase enzyme from whiting fish gill tissue was partially purified for the first time in the literature and the effects of heavy metal compounds on enzyme activity were determined. The purification process was carried out in three stages as homogenate preparation, ammonium sulfate precipitation and also dialysis. In conclusion the study, optimum level pH 7.0, optimal substrate concentration 2 mM NADPH and optimum buffer 150 mM KH2PO4 were determined. After partial purification, the inhibition effects of Cd2, Ni2, Zn2 as heavy metal ions were investigated. The IC50 levels of heavy metals were calculated as 20.17 µM, 33.7 µM and 59.31 µM, respectively.

 Pollution in the seas basically accumulates in marine organisms. Heavy metal residues in aquatic ecosystems can pass through food and cause toxic effects and accumulations in human health. Glutathione reductase GR, which is among the basic enzymes, has an important place in the suppression of stress in the cell. In this study, glutathione reductase enzyme from whiting fish gill tissue was partially purified for the first time in the literature and the effects of heavy metal compounds on enzyme activity were determined. The purification process was carried out in three stages as homogenate preparation, ammonium sulfate precipitation and also dialysis. In conclusion the study, optimum level pH 7.0, optimal substrate concentration 2 mM NADPH and optimum buffer 150 mM KH2PO4 were determined. After partial purification, the inhibition effects of Cd2, Ni2, Zn2 as heavy metal ions were investigated. The IC50 levels of heavy metals were calculated as 20.17 µM, 33.7 µM and 59.31 µM, respectively.

Makale detayları

Purification of Glutathione Reductase Enzyme from Whiting Fish Gill Tissue and Investigation of Metal Inhibition

Yazarlar:

Seyit Batuhan Dudak | Kübra Işık | Betül Mitrovica | Ercan Soydan | Deniz Ekinci

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 6, pp.610-614

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001303237

pISSN:

2618-6578

Enzyme inhibition

Purification

Glutathione reductase

Heavy metals

Merlangius euxmus

Sayı

Makale detayları

Purification of Glutathione Reductase Enzyme from Whiting Fish Gill Tissue and Investigation of Metal Inhibition

Yazarlar: Seyit Batuhan Dudak | Kübra Işık | Betül Mitrovica | Ercan Soydan | Deniz Ekinci

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Ziraat Mühendisliği (Diğer)

Dergi: Black Sea Journal of Agriculture Vol.6 Issue 6, pp.610-614

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001303237

pISSN: 2618-6578

Enzyme inhibition

Purification

Glutathione reductase

Heavy metals

Merlangius euxmus

Sayı

Yazarlar:

Seyit Batuhan Dudak | Kübra Işık | Betül Mitrovica | Ercan Soydan | Deniz Ekinci

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 6, pp.610-614

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001303237

pISSN:

2618-6578