Determination of Free Fatty Acid Composition in Plasma Membranes of Neutrophils in Diabetics

M. Sait KELEŞ

Makale detayları

Determination of Free Fatty Acid Composition in Plasma Membranes of Neutrophils in Diabetics

Yazarlar:

M. Sait KELEŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 5, pp.465-468

Belge Tipi:

Makale

Yayın Tarihi:

4/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757539

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Since frequent and serious  infections occur in diabetic patients, the  investigation of the function of neutrophils is  of importance. Any change in the lipid  composition of the plasma membrane could  be one of the causes of these infections. In  order to investigate this possibility and to  determine any abnormalities, neutrophils and  monocytes were separated by the Histopaque-  1119 method from the blood samples (20 ml)  of 10 diabetic and 10 healthy persons. After  disruption of the cells, membrane lipids and  proteins were isolated. Free fatty acids of  phospholipids in membrane lipids were  isolated by hydrolysing with phospholipase B  under an ultrasonic dismembranater. Free  fatty acids were identified by gas  chromatography at the chloroform phase. The  results were compared with chromatogram of  the standardised free fatty acids. It was  established that there is a statistically  significant difference in the plasma membrane  free fatty acid composition of neutrophils  between the patients and the control group  (p.

 

Since frequent and serious  infections occur in diabetic patients, the  investigation of the function of neutrophils is  of importance. Any change in the lipid  composition of the plasma membrane could  be one of the causes of these infections. In  order to investigate this possibility and to  determine any abnormalities, neutrophils and  monocytes were separated by the Histopaque-  1119 method from the blood samples (20 ml)  of 10 diabetic and 10 healthy persons. After  disruption of the cells, membrane lipids and  proteins were isolated. Free fatty acids of  phospholipids in membrane lipids were  isolated by hydrolysing with phospholipase B  under an ultrasonic dismembranater. Free  fatty acids were identified by gas  chromatography at the chloroform phase. The  results were compared with chromatogram of  the standardised free fatty acids. It was  established that there is a statistically  significant difference in the plasma membrane  free fatty acid composition of neutrophils  between the patients and the control group  (p.

Makale detayları

Determination of Free Fatty Acid Composition in Plasma Membranes of Neutrophils in Diabetics

Yazarlar: M. Sait KELEŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2000 Vol.30 Issue 5, pp.465-468

Belge Tipi: Makale

Yayın Tarihi: 4/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000757539

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

plasma membrane

phospholipase B

free fatty acid

neutrophil

Sayı

Yazarlar:

M. Sait KELEŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 5, pp.465-468

Belge Tipi:

Makale

Yayın Tarihi:

4/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757539

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144