Proteomic analysis of heterotic seed germination in maize using F1 hybrid DHM 117 and  its parental inbreds

Rajesh Kumar MEENA; Durgeshwar PULLAIAHGARI; Padmaja GUDIPALLI

Makale detayları

Proteomic analysis of heterotic seed germination in maize using F1 hybrid DHM 117 and its parental inbreds

Yazarlar:

Rajesh Kumar MEENA | Durgeshwar PULLAIAHGARI | Padmaja GUDIPALLI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 4, pp.352-370

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811218

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 


 
                         A number of differentially expressed proteins (DEPs) were identified in comparative two-dimensional gel electrophoresis
analysis of dry and 24-h water-imbibed seeds of maize F1 hybrid DHM 117 (BML 6 x BML 7) and its parental inbreds. Of the DEPs,
53.4% (86/161) in dry seeds and 58% (127/219) in water-imbibed seeds exhibited a nonadditive pattern in the F1 hybrid as compared
to parental inbreds. A total of 30 DEPs were categorized into different biological processes, most of which were related to metabolism
and energy (34%), followed by storage proteins (27%), stress response (23%), transcription and translation (7%), cell cycle (3%), and
hormone biosynthesis (3%). The transcript accumulation pattern of 8 selected genes corresponding to DEPs was examined using qRTPCR.
Interestingly, LEA protein Rab28 showed higher accumulation in dry seeds at both protein and transcript levels, whereas indole-
3-acetaldehyde oxidase showed lower accumulation in water-imbibed seeds of the F1 hybrid than the female parent at the protein level.
Thus, the DEPs particularly involved in metabolic and energy processes, as well as hormone biosynthesis in the F1 hybrid, might be
responsible for heterotic seed germination in the F1 hybrid. The DEPs identified in this study provide a scope for improving the seed
germination trait of agricultural crops.
                    

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Makale detayları

Proteomic analysis of heterotic seed germination in maize using F1 hybrid DHM 117 and its parental inbreds

Yazarlar: Rajesh Kumar MEENA | Durgeshwar PULLAIAHGARI | Padmaja GUDIPALLI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2018 Vol.42 Issue 4, pp.352-370

Belge Tipi: Makale

Yayın Tarihi: 8/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000811218

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Rajesh Kumar MEENA | Durgeshwar PULLAIAHGARI | Padmaja GUDIPALLI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 4, pp.352-370

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811218

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152