Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR

Başak Oksay; Kadir Serdar Diker

Makale detayları

Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR

Yazarlar:

Başak Oksay | Kadir Serdar Diker

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Mikrobiyolojisi | Veteriner Cerrahi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 1, pp.21-26

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282253

pISSN:

2146-7188

Anahtar kelimeler:

Özet:

 Genital Mycoplasmas Mycoplasma spp. and Ureaplasma diversum, commensals of the genital tract, cause economic loss in cattle enterprises by mainly reproductive failure at the farm level. The productivity loss is due to genetic and environmental factors, stress, wrong antibiotic treatment, and immune system failure. This study aimed to determine the presence of bovine genital mycoplasma and ureaplasmas in healthy and suspected infertile cattle by PCR for the first time. Conventional methods are also used to examine test results. A comparison of results was conducted in both tested methods. For this aim, 356 bovine genital samples of healthy and suspected infertile cows and bulls of various herds were collected from Ankara and the provinces of Türkiye. Positivity in the genital sample of 221 healthy and 135 suspected infertile cattle was 61.2 by cultural methods and 67.7 by PCR, respectively. In comparison to culture, although PCR gave accurate results in a shorter time, findings show that proper results may be obtained when both methods are used together.

 Genital Mycoplasmas Mycoplasma spp. and Ureaplasma diversum, commensals of the genital tract, cause economic loss in cattle enterprises by mainly reproductive failure at the farm level. The productivity loss is due to genetic and environmental factors, stress, wrong antibiotic treatment, and immune system failure. This study aimed to determine the presence of bovine genital mycoplasma and ureaplasmas in healthy and suspected infertile cattle by PCR for the first time. Conventional methods are also used to examine test results. A comparison of results was conducted in both tested methods. For this aim, 356 bovine genital samples of healthy and suspected infertile cows and bulls of various herds were collected from Ankara and the provinces of Türkiye. Positivity in the genital sample of 221 healthy and 135 suspected infertile cattle was 61.2 by cultural methods and 67.7 by PCR, respectively. In comparison to culture, although PCR gave accurate results in a shorter time, findings show that proper results may be obtained when both methods are used together.

 Genital Mycoplasmas Mycoplasma spp. and Ureaplasma diversum, commensals of the genital tract, cause economic loss in cattle enterprises by mainly reproductive failure at the farm level. The productivity loss is due to genetic and environmental factors, stress, wrong antibiotic treatment, and immune system failure. This study aimed to determine the presence of bovine genital mycoplasma and ureaplasmas in healthy and suspected infertile cattle by PCR for the first time. Conventional methods are also used to examine test results. A comparison of results was conducted in both tested methods. For this aim, 356 bovine genital samples of healthy and suspected infertile cows and bulls of various herds were collected from Ankara and the provinces of Türkiye. Positivity in the genital sample of 221 healthy and 135 suspected infertile cattle was 61.2 by cultural methods and 67.7 by PCR, respectively. In comparison to culture, although PCR gave accurate results in a shorter time, findings show that proper results may be obtained when both methods are used together.

 Genital sistem kommensalleri olan genital mikoplazmalar Mycoplasma spp. ve Ureaplasma diversum büyükbaş hayvan işletmelerinde özellikle çiftlik düzeyinde üreme yetersizliği ile ekonomik kayıplara neden olmaktadır. Verimlilik kaybının başlıca nedenleri genetik ve çevresel faktörler, stres, yanlış antibiyotik tedavisi ve bağışıklık sistemi yetmezliği ile ilişkilidir. Bu çalışmanın amacı, sağlıklı ve infertil olduğundan şüphelenilen sığırlarda sığır genital mikoplazma ve üreplazmaların varlığının ilk defa PCR ile belirlenmesidir. Test sonuçları ayrıca geleneksel yöntemlerle de incelenmiştir. Test edilen her iki yöntemde de sonuçların bir karşılaştırması yapılmıştır. Bu amaçla, Ankara ve Türkiyenin illerinden çeşitli sürülere ait sağlıklı ve kısır olduğundan şüphelenilen inekler ve boğalardan toplam 356 adet sığır genital örneği toplandı. 221 sağlıklı ve 135 şüpheli infertil sığırın genital materyalinde pozitiflik sırasıyla kültürel yöntemlerle  61,2 ve PCR ile  67,7 olarak bulundu. Kültüre göre PCR daha kısa sürede doğru sonuçlar vermesine rağmen bulgular her iki yöntemin birlikte kullanılması durumunda uygun sonuçların alınabileceğini gösterdi.

Makale detayları

Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR

Yazarlar:

Başak Oksay | Kadir Serdar Diker

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Mikrobiyolojisi | Veteriner Cerrahi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 1, pp.21-26

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282253

pISSN:

2146-7188

Cattle

Mycoplasmopsis

PCR

Ureaplasma

Büyükbaş hayvan

Mikoplazmopsis

PCR

Üreplazma

Sayı

Makale detayları

Yazarlar: Başak Oksay | Kadir Serdar Diker

Yayıncı: Harran University

Ana Başlıklar: Veteriner Mikrobiyolojisi | Veteriner Cerrahi

Dergi: Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 1, pp.21-26

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001282253

pISSN: 2146-7188

Sayı

Yazarlar:

Başak Oksay | Kadir Serdar Diker

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Mikrobiyolojisi | Veteriner Cerrahi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 1, pp.21-26

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282253

pISSN:

2146-7188