EFFECT OF SOLVENT ON AFLATOXIN CONTENT IN EXTRACTS OF VIRGINIA TYPE PEANUT SKINS

Paul J. SARNOSKİ; Vanessa E. BİLLİNGSLEY; Jodie V. JOHNSON; Sean F. O'KEEFE

doi:https://doi.org/10.3153/JFHS15007

Makale detayları

EFFECT OF SOLVENT ON AFLATOXIN CONTENT IN EXTRACTS OF VIRGINIA TYPE PEANUT SKINS

Yazarlar:

Paul J. SARNOSKİ | Vanessa E. BİLLİNGSLEY | Jodie V. JOHNSON | Sean F. O'KEEFE

Dergi:

Food and Health 2015 Vol.1 Issue 2, pp.75-83

Belge Tipi:

Makale

Yayın Tarihi:

2/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000420160

DOI:

https://doi.org/10.3153/JFHS15007

Özet:

 


Peanut
skins are currently a waste product that may be a potential source of flavonoid
rich compounds. In order to make use of these compounds, an extract would need
to be produced. One of the potential issues with a peanut skin extract could be
that any aflatoxins in the whole skins may be concentrated by solvent
extraction. A
multistep peanut skin extraction (PSE) method (100% water) and a methanol-water
method (30:70 and 80:20) were used to extract proanthocyanidins from natural
peanut skins samples and from peanut skin samples treated with an aflatoxin
standard to determine the recovery by each extraction procedure. Liquid Chromatography-Mass
Spectrometry (LC-MS) detection was employed for the determination and
quantitation of aflatoxins G2, G1, B2, and B1 in the extracts from each of the
samples. No aflatoxins of
interest were detected in the PSE, 30% methanol aqueous (aq.), and 80% methanol
aq. extract of un-spiked peanut skin samples. For aflatoxin spiked standard
(G2, G1, B2, B1) samples, the PSE method (100% water) recovered the least
aflatoxin with mean recoveries of 1-2%. The 30% methanol aq. procedure yielded
mean recoveries of 17-36%. The 80% methanol aq. extraction method recovered
the most aflatoxin, with mean recoveries of 35-63%. Statistical analysis
revealed significant differences in aflatoxin recovery between the three
extraction methods, indicating that both concentration and type of extraction
solvent play significant roles in extraction of aflatoxin. This study provides
evidence that if aflatoxins are present in the starting material for a
flavonoid rich extract, the aflatoxins will carryover and concentrate in the
resultant extract, especially if a high percentage of methanol is used as the
major extraction solvent.


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Makale detayları

EFFECT OF SOLVENT ON AFLATOXIN CONTENT IN EXTRACTS OF VIRGINIA TYPE PEANUT SKINS

Yazarlar: Paul J. SARNOSKİ | Vanessa E. BİLLİNGSLEY | Jodie V. JOHNSON | Sean F. O'KEEFE

Dergi: Food and Health 2015 Vol.1 Issue 2, pp.75-83

Belge Tipi: Makale

Yayın Tarihi: 2/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000420160

DOI: https://doi.org/10.3153/JFHS15007

Sayı

Yazarlar:

Paul J. SARNOSKİ | Vanessa E. BİLLİNGSLEY | Jodie V. JOHNSON | Sean F. O'KEEFE

Dergi:

Food and Health 2015 Vol.1 Issue 2, pp.75-83

Belge Tipi:

Makale

Yayın Tarihi:

2/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000420160

DOI:

https://doi.org/10.3153/JFHS15007