Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis

Beril GÜLER; Filiz ÖZYILMAZ; Nuray CAN; Ebru TAŞTEKİN; Burcu TOKUÇ

Makale detayları

Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis

Yazarlar:

Beril GÜLER | Filiz ÖZYILMAZ | Nuray CAN | Ebru TAŞTEKİN | Burcu TOKUÇ

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2015 Vol.32 Issue 4, pp.388-396

Belge Tipi:

Makale

Yayın Tarihi:

9/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000759784

Sıklık:

İki Aylık

pISSN:

2146-3123

Özet:

 

Background: Gastrointestinal stromal tumors (GIST) have KIT or platelet-derived growth factor receptor α (PDGFRα) mutations affecting receptor tyrosine kinase activity and do not benefit from classic treatment regimens.     Aims: The aim of this study was to review the algorithm that may be followed for the diagnosis and differential diagnosis in GISTs by investigating the histomorphological parameters and expression characteristics of classical immunohistochemical antibodies used in routine tests in addition to DOG1 expression.     Study Design: Diagnostic accuracy study.     Methods: We reevaluated the histological and immunohistochemical parameters of 37 GISTs. The standard immunohistochemical diagnosis and differential diagnosis panel antibodies (CD117, PDGFRα, CD34, vimentin, desmin, SMA, S-100, and Ki67) were studied on the tumor sections. We also used the popular marker DOG1 antibody with accepted sensitivity for GISTs in recent years and the PDGFRα immune marker for which the benefit in routine practice is discussed.     Results: Classification according to progressive disease risk groups of the 37 cases revealed that 54% were in the high risk, 19% in the moderate risk, 16% in the low risk, 8% in the very low risk and 8% in the no risk group.Cytological atypia, necrosis, mucosal invasion and the Ki67 index were found to be related to the progressive disease risk groups of the tumors (p<0.05).Positive immunoreaction was observed with CD117 and PDGFRα in all GISTs in the study (100%). Positivity with the DOG1 antibody was found in 33 (89%) cases. CD34 was positive in 62% (23) of the cases.     Conclusion: The CD117 antibody still plays a key role in GIST diagnosis. However, the use of DOG1 and PDGFRα antibodies combined with CD117 as sensitive markers can be beneficial.

Makale detayları

Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis

Yazarlar: Beril GÜLER | Filiz ÖZYILMAZ | Nuray CAN | Ebru TAŞTEKİN | Burcu TOKUÇ

Yayıncı: Trakya University

Dergi: Balkan Medical Journal 2015 Vol.32 Issue 4, pp.388-396

Belge Tipi: Makale

Yayın Tarihi: 9/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000759784

Sıklık: İki Aylık

pISSN: 2146-3123

Sayı

Yazarlar:

Beril GÜLER | Filiz ÖZYILMAZ | Nuray CAN | Ebru TAŞTEKİN | Burcu TOKUÇ

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2015 Vol.32 Issue 4, pp.388-396

Belge Tipi:

Makale

Yayın Tarihi:

9/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000759784

Sıklık:

İki Aylık

pISSN:

2146-3123