Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test

Mudassar AZHAR; Ahsana Dar FAROOQ; Sayedul HAQUE; Samina BANO; Lubna ZAHEER; Shaheen FAIZI

Makale detayları

Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test

Yazarlar:

Mudassar AZHAR | Ahsana Dar FAROOQ | Sayedul HAQUE | Samina BANO | Lubna ZAHEER | Shaheen FAIZI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2019 Vol.43 Issue 5, pp.326-339

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000811076

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Tagetes patula is used to treat cancer patients in alternative healthcare systems. However, its cytotoxic and genotoxic effects  have not been reported. Therefore, the methanol extract of T. patula flower, the ethyl acetate fraction, and the pure compound patuletin  were evaluated using the Allium test. The methanol extract and fraction contained ~3% and ~36% patuletin, respectively, with ~98%  purity. The methanol extract caused inhibition of Allium root growth displaying an IC50 value of ~500 μg/mL, while the fraction and  patuletin were more potent by ~2 and ~5 times, respectively. The Allium root tips demonstrated a decline in prophase, metaphase,  anaphase, and telophase stages with concomitant decrease in percent mitotic index in the methanol extract (~5.64), fraction, and  patuletin (~4) as compared to the control (~7.61). However, in only methanol extract-treated root tips, an increase in metaphase stage  was noted. In addition, the methanol extract predominantly induced c-type, misaligned, and multipolar chromosomal abnormalities  while the fraction and patuletin displayed fragments and sticky chromosomes. The fraction and patuletin also produced micronuclei  (~2%). In conclusion, T. patula flower methanol extract and ethyl acetate fraction are cytotoxic and genotoxic, which most likely could  be due to the patuletin. Further preclinical and clinical studies are required to justify its clinical use.

Makale detayları

Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test

Yazarlar: Mudassar AZHAR | Ahsana Dar FAROOQ | Sayedul HAQUE | Samina BANO | Lubna ZAHEER | Shaheen FAIZI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2019 Vol.43 Issue 5, pp.326-339

Belge Tipi: Makale

Yayın Tarihi: 9/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000811076

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Mudassar AZHAR | Ahsana Dar FAROOQ | Sayedul HAQUE | Samina BANO | Lubna ZAHEER | Shaheen FAIZI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2019 Vol.43 Issue 5, pp.326-339

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000811076

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152