Dose Dependent Changes In The Crista  Neuroepitheium Resulting From Gentamicin  Ototoxicity In The Chinchilla

Hasan TANYERİ

Makale detayları

Dose Dependent Changes In The Crista Neuroepitheium Resulting From Gentamicin Ototoxicity In The Chinchilla

Yazarlar:

Hasan TANYERİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 1999 Vol.29 Issue 5, pp.545-550

Belge Tipi:

Makale

Yayın Tarihi:

4/1999

Belge Dili:

Türkçe

Document ID (Org No.):

2000757861

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

This experiment was conducted to investigate the dose-dependent effects of gentamicin (GM) on the neuroepithelial morphology of the posterior crista ampullares (PCA) of the adult chinchilla. The experiment focused on hair cell (HC) morphology to monitor GM's dose-dependent effects and also to define an ototoxically-efficient and safe dose what would subsequently be used in a time course study. Chinchillas (n=13) were treated with subcutaneous (SC) daily doses of 120 mg/kg, 60 mg/kg, and 30 mg/kg GM, respectively, for six days. Light microscopy observations were made from the excised PCA of two animals from each group that survided the complete treatment regimen plus one day   post-treatment (PT); these were compared to an untreated control group (n=4). Nephrotoxicity of GM was established by   blood urea nitrogen (BUN) and creatinine measurements. The 120 and 60 mg/kg doses yielded a higher deterioration of kidney function with elevated levels of BUN and creatinine; pathological morphology within the kidneys was also observed. These findings were incompatible with the survival of the animals. In addition, results from one day post-treatment at each of the three different doses revealed non-specific morphological changes such as fusion of the hairs of the cells, shrunken cytoplasm and blebs. These results led us to choose the lower, safer, yet ototoxically-efficient dose of   30 mg/kg to study the time course of the GM-induced effects in a second experiment.

 

This experiment was conducted to investigate the dose-dependent effects of gentamicin (GM) on the neuroepithelial morphology of the posterior crista ampullares (PCA) of the adult chinchilla. The experiment focused on hair cell (HC) morphology to monitor GM's dose-dependent effects and also to define an ototoxically-efficient and safe dose what would subsequently be used in a time course study. Chinchillas (n=13) were treated with subcutaneous (SC) daily doses of 120 mg/kg, 60 mg/kg, and 30 mg/kg GM, respectively, for six days. Light microscopy observations were made from the excised PCA of two animals from each group that survided the complete treatment regimen plus one day   post-treatment (PT); these were compared to an untreated control group (n=4). Nephrotoxicity of GM was established by   blood urea nitrogen (BUN) and creatinine measurements. The 120 and 60 mg/kg doses yielded a higher deterioration of kidney function with elevated levels of BUN and creatinine; pathological morphology within the kidneys was also observed. These findings were incompatible with the survival of the animals. In addition, results from one day post-treatment at each of the three different doses revealed non-specific morphological changes such as fusion of the hairs of the cells, shrunken cytoplasm and blebs. These results led us to choose the lower, safer, yet ototoxically-efficient dose of   30 mg/kg to study the time course of the GM-induced effects in a second experiment.

Makale detayları

Dose Dependent Changes In The Crista Neuroepitheium Resulting From Gentamicin Ototoxicity In The Chinchilla

Yazarlar:

Hasan TANYERİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 1999 Vol.29 Issue 5, pp.545-550

Belge Tipi:

Makale

Yayın Tarihi:

4/1999

Belge Dili:

Türkçe

Document ID (Org No.):

2000757861

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Hair cells

gentamicin ototoxicity

nephrotoxicity

crista ampullaris

chinchilla

Sayı

Makale detayları

Dose Dependent Changes In The Crista Neuroepitheium Resulting From Gentamicin Ototoxicity In The Chinchilla

Yazarlar: Hasan TANYERİ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 1999 Vol.29 Issue 5, pp.545-550

Belge Tipi: Makale

Yayın Tarihi: 4/1999

Belge Dili: Türkçe

Document ID (Org No.): 2000757861

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Hair cells

gentamicin ototoxicity

nephrotoxicity

crista ampullaris

chinchilla

Sayı

Yazarlar:

Hasan TANYERİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 1999 Vol.29 Issue 5, pp.545-550

Belge Tipi:

Makale

Yayın Tarihi:

4/1999

Belge Dili:

Türkçe

Document ID (Org No.):

2000757861

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144