THE EFFECTS OF MELATONIN, FLUOXETINE AND THEIR COMBINATIONS ON STRESS INDUCED BEHAVIORAL AND COGNITIVE IMPAIRMENTS IN MICE

Merve İnci Çamçi; Meral Erdinç; İlker Kelle; Emre Uyar; Levent Erdinç

Makale detayları

THE EFFECTS OF MELATONIN, FLUOXETINE AND THEIR COMBINATIONS ON STRESS INDUCED BEHAVIORAL AND COGNITIVE IMPAIRMENTS IN MICE

Yazarlar:

Merve İnci Çamçi | Meral Erdinç | İlker Kelle | Emre Uyar | Levent Erdinç

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 1, pp.120-132

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281531

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: Melatonin Mel is a hormone with anti-depressant and anti-oxidant features. It is well known that melatonin protects brain cells from reactive oxygen species and that the brains high oxygen consumption and lipid content make it particularly vulnerable to oxidative stress caused by prolonged stress. This study aims to investigate the effects of melatonin, fluoxetine and their combinations on emotional memory, depression, and anxiety-like behavioral changes induced by immobilization Imb stress. Material and Method: 48 male Balbc mice were divided into eight groups: Cnt control, Imb, ImbMlt, ImbFlx fluoxetine, ImbMltFlx, Mlt, Flx and MltFlx. For seven days in a row, the mice underwent daily immobilization stress for 6 hours. Mice were treated with Mlt 10 mgkg and Flx 20 mgkg. All animals were subjected to the behavioral tests; forced swimming test FST, open field test OFT, elevated plus maze EPM, passive avoidance test PAT and hot plate HP test. After all behavioral tests, brain tissues were obtained for malondialdehyde level analysis. Result and Discussion: OFT test data showed the time spent in the central zone and the number of entrances to the central area were significantly lower in the Imb group compared to the Cnt group, these were higher in the ImbFlx, ImbMlt, ImbMltFlx groups compared to the Imb group. Also, according to the data obtained from FST, immobile time was significantly higher in the Imb group compared to the Cnt group, it was lower in the ImbFlx, ImbMlt, ImbMltFlx groups compared to the Imb group. Besides, it was demonstrated that the emotional memory index was statistically higher in the Imb group compared to the Cnt group, and the increasing of memory index returned to normal in the ImbMlt and ImbMltFlx groups with PAT. And also, lipid peroxidation level, which increased in the Imb group, decreased significantly in the ImbFlx, ImbMlt, and ImbMltFlx groups. As a result, it was observed that melatonin has anti-depressant, anxiolytic, antioxidant effects and normalized emotional memory. Also, melatonin, fluoxetine and their combinations exert similar effects in the present study.

 Objective: Melatonin Mel is a hormone with anti-depressant and anti-oxidant features. It is well known that melatonin protects brain cells from reactive oxygen species and that the brains high oxygen consumption and lipid content make it particularly vulnerable to oxidative stress caused by prolonged stress. This study aims to investigate the effects of melatonin, fluoxetine and their combinations on emotional memory, depression, and anxiety-like behavioral changes induced by immobilization Imb stress. Material and Method: 48 male Balbc mice were divided into eight groups: Cnt control, Imb, ImbMlt, ImbFlx fluoxetine, ImbMltFlx, Mlt, Flx and MltFlx. For seven days in a row, the mice underwent daily immobilization stress for 6 hours. Mice were treated with Mlt 10 mgkg and Flx 20 mgkg. All animals were subjected to the behavioral tests; forced swimming test FST, open field test OFT, elevated plus maze EPM, passive avoidance test PAT and hot plate HP test. After all behavioral tests, brain tissues were obtained for malondialdehyde level analysis. Result and Discussion: OFT test data showed the time spent in the central zone and the number of entrances to the central area were significantly lower in the Imb group compared to the Cnt group, these were higher in the ImbFlx, ImbMlt, ImbMltFlx groups compared to the Imb group. Also, according to the data obtained from FST, immobile time was significantly higher in the Imb group compared to the Cnt group, it was lower in the ImbFlx, ImbMlt, ImbMltFlx groups compared to the Imb group. Besides, it was demonstrated that the emotional memory index was statistically higher in the Imb group compared to the Cnt group, and the increasing of memory index returned to normal in the ImbMlt and ImbMltFlx groups with PAT. And also, lipid peroxidation level, which increased in the Imb group, decreased significantly in the ImbFlx, ImbMlt, and ImbMltFlx groups. As a result, it was observed that melatonin has anti-depressant, anxiolytic, antioxidant effects and normalized emotional memory. Also, melatonin, fluoxetine and their combinations exert similar effects in the present study.

 Objective: Melatonin Mel is a hormone with anti-depressant and anti-oxidant features. It is well known that melatonin protects brain cells from reactive oxygen species and that the brains high oxygen consumption and lipid content make it particularly vulnerable to oxidative stress caused by prolonged stress. This study aims to investigate the effects of melatonin, fluoxetine and their combinations on emotional memory, depression, and anxiety-like behavioral changes induced by immobilization Imb stress. Material and Method: 48 male Balbc mice were divided into eight groups: Cnt control, Imb, ImbMlt, ImbFlx fluoxetine, ImbMltFlx, Mlt, Flx and MltFlx. For seven days in a row, the mice underwent daily immobilization stress for 6 hours. Mice were treated with Mlt 10 mgkg and Flx 20 mgkg. All animals were subjected to the behavioral tests; forced swimming test FST, open field test OFT, elevated plus maze EPM, passive avoidance test PAT and hot plate HP test. After all behavioral tests, brain tissues were obtained for malondialdehyde level analysis. Result and Discussion: OFT test data showed the time spent in the central zone and the number of entrances to the central area were significantly lower in the Imb group compared to the Cnt group, these were higher in the ImbFlx, ImbMlt, ImbMltFlx groups compared to the Imb group. Also, according to the data obtained from FST, immobile time was significantly higher in the Imb group compared to the Cnt group, it was lower in the ImbFlx, ImbMlt, ImbMltFlx groups compared to the Imb group. Besides, it was demonstrated that the emotional memory index was statistically higher in the Imb group compared to the Cnt group, and the increasing of memory index returned to normal in the ImbMlt and ImbMltFlx groups with PAT. And also, lipid peroxidation level, which increased in the Imb group, decreased significantly in the ImbFlx, ImbMlt, and ImbMltFlx groups. As a result, it was observed that melatonin has anti-depressant, anxiolytic, antioxidant effects and normalized emotional memory. Also, melatonin, fluoxetine and their combinations exert similar effects in the present study.

 Objective: Melatonin Mel is a hormone with anti-depressant and anti-oxidant features. It is well known that melatonin protects brain cells from reactive oxygen species and that the brains high oxygen consumption and lipid content make it particularly vulnerable to oxidative stress caused by prolonged stress. This study aims to investigate the effects of melatonin, fluoxetine and their combinations on emotional memory, depression, and anxiety-like behavioral changes induced by immobilization Imb stress. Material and Method: 48 male Balbc mice were divided into eight groups: Cnt control, Imb, ImbMlt, ImbFlx fluoxetine, ImbMltFlx, Mlt, Flx and MltFlx. For seven days in a row, the mice underwent daily immobilization stress for 6 hours. Mice were treated with Mlt 10 mgkg and Flx 20 mgkg. All animals were subjected to the behavioral tests; forced swimming test FST, open field test OFT, elevated plus maze EPM, passive avoidance test PAT and hot plate HP test. After all behavioral tests, brain tissues were obtained for malondialdehyde level analysis. Result and Discussion: OFT test data showed the time spent in the central zone and the number of entrances to the central area were significantly lower in the Imb group compared to the Cnt group, these were higher in the ImbFlx, ImbMlt, ImbMltFlx groups compared to the Imb group. Also, according to the data obtained from FST, immobile time was significantly higher in the Imb group compared to the Cnt group, it was lower in the ImbFlx, ImbMlt, ImbMltFlx groups compared to the Imb group. Besides, it was demonstrated that the emotional memory index was statistically higher in the Imb group compared to the Cnt group, and the increasing of memory index returned to normal in the ImbMlt and ImbMltFlx groups with PAT. And also, lipid peroxidation level, which increased in the Imb group, decreased significantly in the ImbFlx, ImbMlt, and ImbMltFlx groups. As a result, it was observed that melatonin has anti-depressant, anxiolytic, antioxidant effects and normalized emotional memory. Also, melatonin, fluoxetine and their combinations exert similar effects in the present study.

Makale detayları

THE EFFECTS OF MELATONIN, FLUOXETINE AND THEIR COMBINATIONS ON STRESS INDUCED BEHAVIORAL AND COGNITIVE IMPAIRMENTS IN MICE

Yazarlar:

Merve İnci Çamçi | Meral Erdinç | İlker Kelle | Emre Uyar | Levent Erdinç

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 1, pp.120-132

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281531

pISSN:

1015-3918

Anxiety

depression

emotional memory

melatonin

stress

Sayı

Makale detayları

THE EFFECTS OF MELATONIN, FLUOXETINE AND THEIR COMBINATIONS ON STRESS INDUCED BEHAVIORAL AND COGNITIVE IMPAIRMENTS IN MICE

Yazarlar: Merve İnci Çamçi | Meral Erdinç | İlker Kelle | Emre Uyar | Levent Erdinç

Yayıncı: Ankara University

Ana Başlıklar: Eczacılık ve İlaç Bilimleri

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 1, pp.120-132

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001281531

pISSN: 1015-3918

Anxiety

depression

emotional memory

melatonin

stress

Sayı

Yazarlar:

Merve İnci Çamçi | Meral Erdinç | İlker Kelle | Emre Uyar | Levent Erdinç

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 1, pp.120-132

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281531

pISSN:

1015-3918