Alternative day fasting protocol attenuates high fructose-induced activation of the TGF-beta/Smad signaling pathway

Gülşah Gündoğdu; Özgen Kılıç Erkek; Ezgi Duman

Makale detayları

Alternative day fasting protocol attenuates high fructose-induced activation of the TGF-beta/Smad signaling pathway

Yazarlar:

Gülşah Gündoğdu | Özgen Kılıç Erkek | Ezgi Duman

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 1, pp.167-178

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318853

pISSN:

1308-0865

Anahtar kelimeler:

Özet:

 Purpose: This study aims to explore the protective effects of alternate-day fasting ADF against metabolic disturbances induced by high fructose HF intake, with a particular focus on modulating the transforming growth factor-beta1 TGF-β1  mother against decapentaplegic homolog 2 Smad2 signaling pathway. Materials and methods: Four groups of rats n7 per group were included: Control, ADF, HF 20 fructose in drinking water, and HFADF. The ADF protocol was applied with 24 hours of ad libitum feeding followed by 24 hours of fasting over a 5-week period. After five weeks, body weight BW, muscle, and fat mass were measured. Serum samples were analyzed using ELISA to assess levels of TGF-β1, Smad2, connective tissue growth factor CTGF, and total oxidant-antioxidant status TOS-TAS. Results: Results indicated that that HF significantly increased final BW, and ADF reduced this weight gain p0.001. ADF also led to lower gastrocnemius-soleus muscle weights compared to controls p0.001, but mitigated fructose-induced retroperitoneal fat accumulation. TAS levels were higher, and TOS levels were lower in the ADF groups, showing an antioxidant shift p0.05. Moreover, ADF significantly attenuated the TGF-β1 Smad2 pathway activation by decreasing serum TGF-β1, Smad2, and CTGF levels p0.05, suggesting a protective role against fructose-induced metabolic dysregulation. Conclusions: These findings suggest that ADF could be an effective dietary intervention for mitigating the metabolic impact of excessive fructose intake, particularly by regulating oxidative stress and the TGF-β1Smad2 pathway.

 Purpose: This study aims to explore the protective effects of alternate-day fasting ADF against metabolic disturbances induced by high fructose HF intake, with a particular focus on modulating the transforming growth factor-beta1 TGF-β1  mother against decapentaplegic homolog 2 Smad2 signaling pathway. Materials and methods: Four groups of rats n7 per group were included: Control, ADF, HF 20 fructose in drinking water, and HFADF. The ADF protocol was applied with 24 hours of ad libitum feeding followed by 24 hours of fasting over a 5-week period. After five weeks, body weight BW, muscle, and fat mass were measured. Serum samples were analyzed using ELISA to assess levels of TGF-β1, Smad2, connective tissue growth factor CTGF, and total oxidant-antioxidant status TOS-TAS. Results: Results indicated that that HF significantly increased final BW, and ADF reduced this weight gain p0.001. ADF also led to lower gastrocnemius-soleus muscle weights compared to controls p0.001, but mitigated fructose-induced retroperitoneal fat accumulation. TAS levels were higher, and TOS levels were lower in the ADF groups, showing an antioxidant shift p0.05. Moreover, ADF significantly attenuated the TGF-β1 Smad2 pathway activation by decreasing serum TGF-β1, Smad2, and CTGF levels p0.05, suggesting a protective role against fructose-induced metabolic dysregulation. Conclusions: These findings suggest that ADF could be an effective dietary intervention for mitigating the metabolic impact of excessive fructose intake, particularly by regulating oxidative stress and the TGF-β1Smad2 pathway.

 Purpose: This study aims to explore the protective effects of alternate-day fasting ADF against metabolic disturbances induced by high fructose HF intake, with a particular focus on modulating the transforming growth factor-beta1 TGF-β1  mother against decapentaplegic homolog 2 Smad2 signaling pathway. Materials and methods: Four groups of rats n7 per group were included: Control, ADF, HF 20 fructose in drinking water, and HFADF. The ADF protocol was applied with 24 hours of ad libitum feeding followed by 24 hours of fasting over a 5-week period. After five weeks, body weight BW, muscle, and fat mass were measured. Serum samples were analyzed using ELISA to assess levels of TGF-β1, Smad2, connective tissue growth factor CTGF, and total oxidant-antioxidant status TOS-TAS. Results: Results indicated that that HF significantly increased final BW, and ADF reduced this weight gain p0.001. ADF also led to lower gastrocnemius-soleus muscle weights compared to controls p0.001, but mitigated fructose-induced retroperitoneal fat accumulation. TAS levels were higher, and TOS levels were lower in the ADF groups, showing an antioxidant shift p0.05. Moreover, ADF significantly attenuated the TGF-β1 Smad2 pathway activation by decreasing serum TGF-β1, Smad2, and CTGF levels p0.05, suggesting a protective role against fructose-induced metabolic dysregulation. Conclusions: These findings suggest that ADF could be an effective dietary intervention for mitigating the metabolic impact of excessive fructose intake, particularly by regulating oxidative stress and the TGF-β1Smad2 pathway.

 Amaç: Bu çalışma, yüksek fruktoz HF alımının yol açtığı metabolik bozukluklara karşı alternatif gün orucu ADF uygulamasının koruyucu etkilerini incelemeyi amaçlamakta olup, özellikle transformasyon büyüme faktörübeta1 TGF-β1  decapentaplegic homolog 2ye karşı ana protein Smad2 sinyal yolunun modülasyonuna odaklanmaktadır. Gereç ve yöntem: Sıçanlar dört grupta her grup için n7 çalışmaya dahil edilmiştir: Kontrol, ADF, HF içme suyunda 20 fruktoz, ve HFADF. ADF protokolü, 5 hafta boyunca 24 saat serbest yem tüketimi ve onu takiben 24 saat açlık olarak gerçekleştirilmiştir. Beş hafta sonunda vücut ağırlığı VA, kas ve yağ kütlesi ölçülmüştür. Serum örneklerinde TGF-β1, Smad2, bağ dokusu büyüme faktörü CTGF ve total oksidan-antioksidan TOSTAS düzeyleri ELISA yöntemi ile analiz edilmiştir. Bulgular: Sonuçlar, HFnin final BWyi anlamlı derecede artırdığını ve ADFnin bu kilo alımını azalttığını göstermiştir p0,001. ADF, kontrol grubuna kıyasla gastrocnemius-soleus kas ağırlıklarını azaltmış p0,001, ancak fruktoz kaynaklı retroperitoneal yağ birikimini hafifletmiştir. ADF gruplarında TAS düzeyleri daha yüksek, TOS düzeyleri ise daha düşük bulunmuş olup, bu da bir antioksidan kaymasına işaret etmiştir p0,05. Ayrıca, ADF, serum TGF-β1, Smad2 ve CTGF seviyelerini düşürerek TGF-β1Smad2 yolunun aktivasyonunu önemli ölçüde azaltmış p0,05 ve fruktoz kaynaklı metabolik düzensizliklere karşı koruyucu bir role işaret etmektedir. Sonuç: Elde edilen veriler ADFnin, özellikle oksidatif stres ve TGF-β1Smad2 yolağını düzenleyerek, aşırı fruktoz alımı ile gerçekleşen metabolik etkileri hafifletmek için etkili bir diyet müdahalesi olabileceğini düşündürmektedir.

Makale detayları

Alternative day fasting protocol attenuates high fructose-induced activation of the TGF-beta/Smad signaling pathway

Yazarlar:

Gülşah Gündoğdu | Özgen Kılıç Erkek | Ezgi Duman

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 1, pp.167-178

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318853

pISSN:

1308-0865

Alternate-day fasting

high-fructose

TGF-β1/Smad2 pathway

oxidative stress

metabolic healthAlternatif günlerde açlık

yüksek fruktoz

TGF-β1/Smad2 yolağı

oksidatif stres

metabolik sağlık

Sayı

Makale detayları

Yazarlar: Gülşah Gündoğdu | Özgen Kılıç Erkek | Ezgi Duman

Yayıncı: Pamukkale University

Ana Başlıklar: Tıbbi Fizyoloji (Diğer)

Dergi: Pamukkale Tıp Dergisi Vol.18 Issue 1, pp.167-178

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001318853

pISSN: 1308-0865

Sayı

Yazarlar:

Gülşah Gündoğdu | Özgen Kılıç Erkek | Ezgi Duman

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 1, pp.167-178

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318853

pISSN:

1308-0865