Evaluation of inhibitory potency of endemic Onion bulbs: Analysis of phenolic compounds and enzyme inhibition with the computational study

Dursun Kısa

Makale detayları

Evaluation of inhibitory potency of endemic Onion bulbs: Analysis of phenolic compounds and enzyme inhibition with the computational study

Yazarlar:

Dursun Kısa

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji | Organik Kimya

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 4, pp.828-836

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294338

pISSN:

2149-3367

Anahtar kelimeler:

Özet:

 Numerous Allium species have been utilized in complementary medicine based on their biological activities. In the present work, the bulb extract of A. kastambulense was studied for its phenolic content and enzyme inhibition ability assisted by computer-aided molecular docking studies. The evaluation of enzyme inhibition activity of the bulb extract showed that it has 54.701.74 134.32.12 IC50 for six studied enzymes, respectively. In the reverse-phase HPLC analysis of methanol-chloroform bulb extract, catechin, chlorogenic acid, gentisic acid, vanillic acid, and apigenin were founded to be the represented group of phenolic components. Catechin flavonoid was extensively detected as the most abundant ingredient, and TPC and TFC were calculated at 6.86  0.36 mg g 1 and 3.22  0.14 mg g 1. Finally, the catechin ligand has low binding energy values against the studied enzymes with -5.778 - -8.872 kcalmol.

 Numerous Allium species have been utilized in complementary medicine based on their biological activities. In the present work, the bulb extract of A. kastambulense was studied for its phenolic content and enzyme inhibition ability assisted by computer-aided molecular docking studies. The evaluation of enzyme inhibition activity of the bulb extract showed that it has 54.701.74 134.32.12 IC50 for six studied enzymes, respectively. In the reverse-phase HPLC analysis of methanol-chloroform bulb extract, catechin, chlorogenic acid, gentisic acid, vanillic acid, and apigenin were founded to be the represented group of phenolic components. Catechin flavonoid was extensively detected as the most abundant ingredient, and TPC and TFC were calculated at 6.86  0.36 mg g 1 and 3.22  0.14 mg g 1. Finally, the catechin ligand has low binding energy values against the studied enzymes with -5.778 - -8.872 kcalmol.

 Numerous Allium species have been utilized in complementary medicine based on their biological activities. In the present work, the bulb extract of A. kastambulense was studied for its phenolic content and enzyme inhibition ability assisted by computer-aided molecular docking studies. The evaluation of enzyme inhibition activity of the bulb extract showed that it has 54.701.74 134.32.12 IC50 for six studied enzymes, respectively. In the reverse-phase HPLC analysis of methanol-chloroform bulb extract, catechin, chlorogenic acid, gentisic acid, vanillic acid, and apigenin were founded to be the represented group of phenolic components. Catechin flavonoid was extensively detected as the most abundant ingredient, and TPC and TFC were calculated at 6.86  0.36 mg g 1 and 3.22  0.14 mg g 1. Finally, the catechin ligand has low binding energy values against the studied enzymes with -5.778 - -8.872 kcalmol.

 Çok sayıda Allium türü, biyolojik aktivitelerine dayalı olarak tamamlayıcı tıpta kullanılmaktadır. Bu çalışmada, A. kastambulense soğan ekstresinin fenolik içeriği ve enzim inhibisyon yeteneği bilgisayar destekli moleküler kenetlenme çalışmaları incelenmiştir. Soğan ekstraktının enzim inhibisyonu aktivitesi, çalışılan altı enzim için 54.701.74 - 134.32.12 aralığında IC50ye sahiptir. Metanol-kloroform soğan ekstresinin ters faz HPLC analizinde kateşin, klorojenik asit, gentisik asit, vanilik asit ve apigenin fenolik bileşenleri temsil eden metabolitler olarak bulunmuştur. Bir flavonoid olan kateşin flavonoid en bol bulunan bileşen olarak tespit edildi. TPC ve TFC değerleri sırasıyla 6.86  0.36 mg g-1 ve 3.22  0.14 mg g-1 olarak hesaplandı. Son olarak, kateşin ligandı, üzerinde çalışılan enzimlere karşı düşük bağlanma enerjisi değerlerine - 5,778 - -8,872 kcalmol sahiptir.

Makale detayları

Evaluation of inhibitory potency of endemic Onion bulbs: Analysis of phenolic compounds and enzyme inhibition with the computational study

Yazarlar:

Dursun Kısa

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji | Organik Kimya

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 4, pp.828-836

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294338

pISSN:

2149-3367

Allium kastambulense

enzyme inhibition

phenolic compound

molecular dockingAllium kastambulense

fenolik bileşik

enzim inhibisyonu

moleküler kenetlenme

Sayı

Makale detayları

Yazarlar: Dursun Kısa

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Yapısal Biyoloji | Organik Kimya

Dergi: Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 4, pp.828-836

Belge Tipi: Makale

Yayın Tarihi: 8/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001294338

pISSN: 2149-3367

Sayı

Yazarlar:

Dursun Kısa

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji | Organik Kimya

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 4, pp.828-836

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294338

pISSN:

2149-3367