Characterisation of an Exopolysaccharide Preventing Phage Adsorption in Lactococcus lactis subsp. cremoris MA39

Mustafa AKÇELİK; Pınar ŞANLIBABA

Makale detayları

Characterisation of an Exopolysaccharide Preventing Phage Adsorption in Lactococcus lactis subsp. cremoris MA39

Yazarlar:

Mustafa AKÇELİK | Pınar ŞANLIBABA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1151-1156

Belge Tipi:

Makale

Yayın Tarihi:

4/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770543

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Lactococcus lactis subsp. cremoris strain MA39 produces an extracellular polysaccharide containing rhamnose, glucose and galactose. A 16.5 kb plasmid encoding exopolysaccharide production in MA39 was determined by plasmid curing experiments. Phage adsorption assays showed that four different lactococcal phages were adsorbed to MA39-40; only the 16.5 kb plasmid cured an exopolysaccharide non-producing mutant of strain MA39, with high efficiency (93.2-98.5%) while adsorption of these phages was completely inhibited in the wild type strain MA39. These results suggest that exopolysaccharide prevents phage adsorption by masking phage receptor sites.

 

Lactococcus lactis subsp. cremoris strain MA39 produces an extracellular polysaccharide containing rhamnose, glucose and galactose. A 16.5 kb plasmid encoding exopolysaccharide production in MA39 was determined by plasmid curing experiments. Phage adsorption assays showed that four different lactococcal phages were adsorbed to MA39-40; only the 16.5 kb plasmid cured an exopolysaccharide non-producing mutant of strain MA39, with high efficiency (93.2-98.5%) while adsorption of these phages was completely inhibited in the wild type strain MA39. These results suggest that exopolysaccharide prevents phage adsorption by masking phage receptor sites.

Makale detayları

Characterisation of an Exopolysaccharide Preventing Phage Adsorption in Lactococcus lactis subsp. cremoris MA39

Yazarlar:

Mustafa AKÇELİK | Pınar ŞANLIBABA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1151-1156

Belge Tipi:

Makale

Yayın Tarihi:

4/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770543

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Exopolycaccharide

phage resistance

Lactococcus lactis subsp. Cremoris

Sayı

Makale detayları

Characterisation of an Exopolysaccharide Preventing Phage Adsorption in Lactococcus lactis subsp. cremoris MA39

Yazarlar: Mustafa AKÇELİK | Pınar ŞANLIBABA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1151-1156

Belge Tipi: Makale

Yayın Tarihi: 4/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000770543

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Exopolycaccharide

phage resistance

Lactococcus lactis subsp. Cremoris

Sayı

Yazarlar:

Mustafa AKÇELİK | Pınar ŞANLIBABA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1151-1156

Belge Tipi:

Makale

Yayın Tarihi:

4/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770543

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128