The Role of Phospholiphase D in Phospholiphid Transaction During Cotton Seeds Maturation

Muborak Mahmusovna ABDULLAEVA; Mamura Muhamedovna MULLADGANOVA

Makale detayları

The Role of Phospholiphase D in Phospholiphid Transaction During Cotton Seeds Maturation

Yazarlar:

Muborak Mahmusovna ABDULLAEVA | Mamura Muhamedovna MULLADGANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 4, pp.241-246

Belge Tipi:

Makale

Yayın Tarihi:

3/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813691

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The role of phospholipase D (EC 3.1.4.4) was studied in the changing of phospholipid content during cotton seed (Gossipium hirsutum 108-G) maturation.    To define phospholipase D activity cotton seed homogenates were incubated in a water-methanol environment at 37 °C for various time intervals (15, 30, 60, 120, and 300 min). Enzyme activity was judged from the formation of hydrolysis products - phosphatidic acid and phosphatidylmethanol. The phospholipid content was defined by using thin-layer chromatography with a follow up analysis of phosphorus quantity in phospholipid fractions.    It was shown that phospholipase D displayed two functions during cotton seed maturation: transferase and hydrolase. Hydrolase function could be defined from the increase of the product (phosphatidic acid) concentration during the complete maturation of the seed. We noticed that the concentration increased. Transferase activity could be defined through the increase of the phosphatidylmetanol concentration during the incubation of phospholipids in a water-methanol environment. The experimental data showed that phospholipase D plays an important role in phospholipid metabolism.

 

The role of phospholipase D (EC 3.1.4.4) was studied in the changing of phospholipid content during cotton seed (Gossipium hirsutum 108-G) maturation.    To define phospholipase D activity cotton seed homogenates were incubated in a water-methanol environment at 37 °C for various time intervals (15, 30, 60, 120, and 300 min). Enzyme activity was judged from the formation of hydrolysis products - phosphatidic acid and phosphatidylmethanol. The phospholipid content was defined by using thin-layer chromatography with a follow up analysis of phosphorus quantity in phospholipid fractions.    It was shown that phospholipase D displayed two functions during cotton seed maturation: transferase and hydrolase. Hydrolase function could be defined from the increase of the product (phosphatidic acid) concentration during the complete maturation of the seed. We noticed that the concentration increased. Transferase activity could be defined through the increase of the phosphatidylmetanol concentration during the incubation of phospholipids in a water-methanol environment. The experimental data showed that phospholipase D plays an important role in phospholipid metabolism.

Makale detayları

The Role of Phospholiphase D in Phospholiphid Transaction During Cotton Seeds Maturation

Yazarlar:

Muborak Mahmusovna ABDULLAEVA | Mamura Muhamedovna MULLADGANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 4, pp.241-246

Belge Tipi:

Makale

Yayın Tarihi:

3/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813691

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Phospholipase D

Phospholipids

Cotton seeds.

Sayı

Makale detayları

The Role of Phospholiphase D in Phospholiphid Transaction During Cotton Seeds Maturation

Yazarlar: Muborak Mahmusovna ABDULLAEVA | Mamura Muhamedovna MULLADGANOVA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2002 Vol.26 Issue 4, pp.241-246

Belge Tipi: Makale

Yayın Tarihi: 3/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000813691

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Phospholipase D

Phospholipids

Cotton seeds.

Sayı

Yazarlar:

Muborak Mahmusovna ABDULLAEVA | Mamura Muhamedovna MULLADGANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 4, pp.241-246

Belge Tipi:

Makale

Yayın Tarihi:

3/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813691

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152