Investigation of Gene Expression Levels of Some Proteins Related to the Pathogenesis of Parkinson's Disease in Rats Exposed to Prenatal Stress

İlayda Varol; Ezgi Turunç

Makale detayları

Investigation of Gene Expression Levels of Some Proteins Related to the Pathogenesis of Parkinson's Disease in Rats Exposed to Prenatal Stress

Yazarlar:

İlayda Varol | Ezgi Turunç

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Issue Special Issue / International Multidisciplinary Symposium on Drug Research and Development, DRD-2023, pp.34-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001323366

pISSN:

2458-8806

Anahtar kelimeler:

Özet:

 IThis study was carried out in order to better understand how prenatal stress PS may affect the future onset of Parkinsons disease PD. A dexamethasone-induced PS model was established in rats for the study. Changes in the expression levels of tyrosine hydroxylase TH, α-synuclein SNCA, dopamine transporter SLC6A3, and parkin PRKN proteins, which play role in PD pathogenesis, were demonstrated by real-time PCR in the cerebral cortex of male rats exposed to PS. From GD 14 to 21, pregnant rats were injected daily with Dex or saline 100 μgkg and 1 mlkg. 3 months after birth, male rats underwent decapitation n5, cerebral cortex dissection was performed. Total RNA was isolated from cortexes and used for cDNA synthesis. Gene expression analyzes were performed according to the ΔΔCT method. The statistical differences between groups were analyzed by the Mann-Whitney test. Statistics were deemed significant at a level of 0.05. Dex exposure throughout pregnancy significantly increased mRNA levels of TH and SLC6A3. No significant differences were found in the mRNA levels of PRKN and SNCA between experimental groups. In conclusion, offspring exposed to PS may be more susceptible to PD in adulthood through changes in the cortical mRNA levels of TH and SLC6A3.

 IThis study was carried out in order to better understand how prenatal stress PS may affect the future onset of Parkinsons disease PD. A dexamethasone-induced PS model was established in rats for the study. Changes in the expression levels of tyrosine hydroxylase TH, α-synuclein SNCA, dopamine transporter SLC6A3, and parkin PRKN proteins, which play role in PD pathogenesis, were demonstrated by real-time PCR in the cerebral cortex of male rats exposed to PS. From GD 14 to 21, pregnant rats were injected daily with Dex or saline 100 μgkg and 1 mlkg. 3 months after birth, male rats underwent decapitation n5, cerebral cortex dissection was performed. Total RNA was isolated from cortexes and used for cDNA synthesis. Gene expression analyzes were performed according to the ΔΔCT method. The statistical differences between groups were analyzed by the Mann-Whitney test. Statistics were deemed significant at a level of 0.05. Dex exposure throughout pregnancy significantly increased mRNA levels of TH and SLC6A3. No significant differences were found in the mRNA levels of PRKN and SNCA between experimental groups. In conclusion, offspring exposed to PS may be more susceptible to PD in adulthood through changes in the cortical mRNA levels of TH and SLC6A3.

 IThis study was carried out in order to better understand how prenatal stress PS may affect the future onset of Parkinsons disease PD. A dexamethasone-induced PS model was established in rats for the study. Changes in the expression levels of tyrosine hydroxylase TH, α-synuclein SNCA, dopamine transporter SLC6A3, and parkin PRKN proteins, which play role in PD pathogenesis, were demonstrated by real-time PCR in the cerebral cortex of male rats exposed to PS. From GD 14 to 21, pregnant rats were injected daily with Dex or saline 100 μgkg and 1 mlkg. 3 months after birth, male rats underwent decapitation n5, cerebral cortex dissection was performed. Total RNA was isolated from cortexes and used for cDNA synthesis. Gene expression analyzes were performed according to the ΔΔCT method. The statistical differences between groups were analyzed by the Mann-Whitney test. Statistics were deemed significant at a level of 0.05. Dex exposure throughout pregnancy significantly increased mRNA levels of TH and SLC6A3. No significant differences were found in the mRNA levels of PRKN and SNCA between experimental groups. In conclusion, offspring exposed to PS may be more susceptible to PD in adulthood through changes in the cortical mRNA levels of TH and SLC6A3.

 Bu çalışma, prenatal stresin PS Parkinson hastalığının PH gelecekteki başlangıcını nasıl etkileyebileceğini daha iyi anlamak amacıyla gerçekleştirilmiştir. Çalışma için sıçanlarda deksametazon ile indüklenen bir PS modeli oluşturulmuştur. PH patogenezinde rol oynayan tirozin hidroksilaz TH, α-sinüklein SNCA, dopamin taşıyıcı SLC6A3 ve parkin PRKN proteinlerinin ekspresyon seviyelerindeki değişiklikler, PSye maruz kalan erkek sıçanların serebral korteksinde gerçek zamanlı PCR ile gösterilmiştir. Hamileliklerinin 14. Gününden 21. Gününe kadar, gebe sıçanlara günlük olarak deksametazon veya salin 100 μgkg ve 1 mlkg enjekte edilmiştir. Doğumdan 3 ay sonra, erkek sıçanlara dekapitasyon uygulandı n5, serebral korteks diseksiyonu yapıldı. Total RNA kortekslerden izole edildi ve cDNA sentezi için kullanıldı. Gen ekspresyon analizleri CT yöntemine göre yapıldı. Gruplar arasındaki istatistiksel farklılıklar Mann-Whitney testi ile analiz edildi. İstatistikler p0.05 düzeyinde anlamlı kabul edildi. Gebelik boyunca deksametazon maruziyeti TH ve SLC6A3 mRNA düzeylerini anlamlı şekilde arttırmıştır. Deney grupları arasında PRKN ve SNCA mRNA seviyelerinde anlamlı bir fark bulunmamıştır. Sonuç olarak, PSye maruz kalan yavrular, TH ve SLC6A3ün kortikal mRNA seviyelerinde meydana gelen değişimlerden dolayı yetişkinlikte PHye daha duyarlı olabilir. Anahtar Kelimeler: Prenatal stres, Parkinson Hastalığı, Tirozin hidroksilaz, Dopamin taşıyıcı.

Makale detayları

Investigation of Gene Expression Levels of Some Proteins Related to the Pathogenesis of Parkinson's Disease in Rats Exposed to Prenatal Stress

Yazarlar:

İlayda Varol | Ezgi Turunç

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Issue Special Issue / International Multidisciplinary Symposium on Drug Research and Development, DRD-2023, pp.34-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001323366

pISSN:

2458-8806

Prenatal Stress

Parkinson's disease

Tyrosine hyrdoxylase

Dopamine transporterPrenatal stres

Parkinson hastalığı

Tirozin hidroksilaz

Dopamin taşıyıcı

Sayı

Makale detayları

Yazarlar: İlayda Varol | Ezgi Turunç

Yayıncı: Hacettepe University

Ana Başlıklar: Pharmaceutical Biochemistry

Dergi: Hacettepe University Journal of the Faculty of Pharmacy Issue Special Issue / International Multidisciplinary Symposium on Drug Research and Development, DRD-2023, pp.34-45

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001323366

pISSN: 2458-8806

Sayı

Yazarlar:

İlayda Varol | Ezgi Turunç

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Issue Special Issue / International Multidisciplinary Symposium on Drug Research and Development, DRD-2023, pp.34-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001323366

pISSN:

2458-8806