A stereological study on calculation of volume values of the cervical spinal segments in ducks

Gamze ÇAKMAK; Hüseyin KARADAĞ; Zafer SOYGÜDER; Murat Çetin RAĞBETLİ; Serkan YILDIRIM; Hacı KELEŞ

Makale detayları

A stereological study on calculation of volume values of the cervical spinal segments in ducks

Yazarlar:

Gamze ÇAKMAK | Hüseyin KARADAĞ | Zafer SOYGÜDER | Murat Çetin RAĞBETLİ | Serkan YILDIRIM | Hacı KELEŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 6, pp.600-610

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764518

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 


 
                         In the present study, volume values of the cervical segments in the spinal cords of adult ducks weighing 3-4 kg were
investigated. Volume values of the grey matter and white matter in the cervical segment of the spinal cord of the ducks were examined
stereologically. Ten ducks (genus Anas) without regarding the sex of the animals. All animals were perfused using 10% formaldehyde.
The animals were kept in 10% formaldehyde for 1 week to ensure fixation. The ducks were then dissected. The cervical segments were
uncovered by removing the cervical vertebrae. Tissue samples were obtained from each of the cervical spinal segments, and 5-μm-thick
cross-sections were obtained from these samples. Sampling was performed at a ratio of 1/250 to obtain 12 cross-sections from each
cervical segment of the animals. These sections were stained using the hematoxylin and eosin staining technique. Photos were taken
under a microscope. Volume values of whole tissue, grey matter, and white matter were calculated for each cervical segment of the spinal
cord of the ducks. Cavalieri's principle was employed for the calculation. As a result, the vertebral column was used as a guide to identify
the cervical segments of the spinal cord. The cervical segments were also obtained by dissection without using a decalcification process.
It was determined that the number of segments was 15. When mean volume values of the whole cervical segment of the spinal cord in
the ducks were evaluated, the highest mean volume was determined as 4.224 mm3    3   33^{3} in segment C15. The cervical spinal segment with the
lowest value of white matter was C7 (0.915 mm3    3   33^{3}). When the volume values of the grey matter of the cervical segments of the ducks were
examined, it was determined that segments C14 and C15 had the highest values, calculated as 0.511 mm3    3   33^{3} and 0.513 mm3    3   33^{3}, respectively.
It was determined that segments C12, C13, C14, and C15 were involved in the cervical enlargement.
                    

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Makale detayları

A stereological study on calculation of volume values of the cervical spinal segments in ducks

Yazarlar: Gamze ÇAKMAK | Hüseyin KARADAĞ | Zafer SOYGÜDER | Murat Çetin RAĞBETLİ | Serkan YILDIRIM | Hacı KELEŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 6, pp.600-610

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000764518

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Gamze ÇAKMAK | Hüseyin KARADAĞ | Zafer SOYGÜDER | Murat Çetin RAĞBETLİ | Serkan YILDIRIM | Hacı KELEŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 6, pp.600-610

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764518

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128