Examining the Impact of 4-Nonylphenol on G-361 Malignant Melanoma Cell Proliferation and Apoptosis Activation

Esra Bilici; Cevdet Uğuz; Korhan Altunbaş

Makale detayları

Examining the Impact of 4-Nonylphenol on G-361 Malignant Melanoma Cell Proliferation and Apoptosis Activation

Yazarlar:

Esra Bilici | Cevdet Uğuz | Korhan Altunbaş

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Van Veterinary Journal Vol.36 Issue 2, pp.110-114

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355488

pISSN:

2149-3359

Anahtar kelimeler:

Özet:

 Nonylphenol NP is among the most prevalent endocrine-disrupting compounds found in the environment. Although the carcinogenic properties of endocrine disruptors have been well investigated, their possible anticancer effects remain comparatively underexplored. This study investigated the cytotoxic and pro-apoptotic effects of NP on G-361 human malignant melanoma cell line. The major objective of the stuyd was to assess anticancer effects of the endocrine-disrupting compound NP on G-361 melanoma cells. The MTT assay was used to determine cell viability, and quantitative real-time PCR was performed to analyze the expression levels of apoptosis-associated genes, including Caspase-3, Bcl-2, Bax, P53, P21, and β-actin. The results indicated a significant decrease in cell viability following treatment with 100 μgml NP. Notably, NP exerted a dose-dependent cytotoxic effect, suggesting its possible role in the modulation of apoptotic pathways in melanoma cells. These results require further investigation of different pathways by which NP exerts its effects to gain a deeper insight into its molecular mechanism.

 Nonylphenol NP is among the most prevalent endocrine-disrupting compounds found in the environment. Although the carcinogenic properties of endocrine disruptors have been well investigated, their possible anticancer effects remain comparatively underexplored. This study investigated the cytotoxic and pro-apoptotic effects of NP on G-361 human malignant melanoma cell line. The major objective of the stuyd was to assess anticancer effects of the endocrine-disrupting compound NP on G-361 melanoma cells. The MTT assay was used to determine cell viability, and quantitative real-time PCR was performed to analyze the expression levels of apoptosis-associated genes, including Caspase-3, Bcl-2, Bax, P53, P21, and β-actin. The results indicated a significant decrease in cell viability following treatment with 100 μgml NP. Notably, NP exerted a dose-dependent cytotoxic effect, suggesting its possible role in the modulation of apoptotic pathways in melanoma cells. These results require further investigation of different pathways by which NP exerts its effects to gain a deeper insight into its molecular mechanism.

 Nonylphenol NP is among the most prevalent endocrine-disrupting compounds found in the environment. Although the carcinogenic properties of endocrine disruptors have been well investigated, their possible anticancer effects remain comparatively underexplored. This study investigated the cytotoxic and pro-apoptotic effects of NP on G-361 human malignant melanoma cell line. The major objective of the stuyd was to assess anticancer effects of the endocrine-disrupting compound NP on G-361 melanoma cells. The MTT assay was used to determine cell viability, and quantitative real-time PCR was performed to analyze the expression levels of apoptosis-associated genes, including Caspase-3, Bcl-2, Bax, P53, P21, and β-actin. The results indicated a significant decrease in cell viability following treatment with 100 μgml NP. Notably, NP exerted a dose-dependent cytotoxic effect, suggesting its possible role in the modulation of apoptotic pathways in melanoma cells. These results require further investigation of different pathways by which NP exerts its effects to gain a deeper insight into its molecular mechanism.

 Nonilfenol NP, çevrede bulunan en yaygın endokrin bozucu bileşikler arasındadır. Endokrin bozucuların kanserojen özellikleri iyi araştırılmış olsa da olası antikanser etkileri nispeten yeterince araştırılmamıştır. Bu çalışma, NPnin G-361 insan malign melanom hücre hattı üzerindeki sitotoksik ve proapoptotik etkilerini araştırmıştır. Çalışmanın temel amacı, endokrin bozucu bileşik NPnin G-361 melanom hücreleri üzerindeki antikanser etkilerini değerlendirmekti. Hücre canlılığını belirlemek için MTT testi kullanıldı ve Caspase-3, Bcl-2, Bax, P53, P21 ve β-actin dahil olmak üzere apoptozla ilişkili genlerin ifade düzeylerini analiz etmek için kantitatif gerçek zamanlı PCR gerçekleştirildi. Sonuçlar, 100 μgml NP ile tedaviden sonra hücre canlılığında önemli bir azalma olduğunu gösterdi. Özellikle, NP doz bağımlı bir sitotoksik etki gösterdi ve bu da melanom hücrelerinde apoptotik yolların modülasyonunda olası bir rolü olduğunu düşündürdü. Bu sonuçlar, NPnin moleküler mekanizmasına daha derin bir bakış açısı kazanmak için etkilerini gösterdiği farklı yolların daha fazla araştırılmasını gerektiriyor.

Makale detayları

Examining the Impact of 4-Nonylphenol on G-361 Malignant Melanoma Cell Proliferation and Apoptosis Activation

Yazarlar:

Esra Bilici | Cevdet Uğuz | Korhan Altunbaş

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Van Veterinary Journal Vol.36 Issue 2, pp.110-114

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355488

pISSN:

2149-3359

P53

Melanoma

Nonylphenol

ProliferationP53

Melanoma

Nonilfenol

Proliferasyon

Sayı

Makale detayları

Yazarlar: Esra Bilici | Cevdet Uğuz | Korhan Altunbaş

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Veteriner Bilimleri (Diğer)

Dergi: Van Veterinary Journal Vol.36 Issue 2, pp.110-114

Belge Tipi: Makale

Yayın Tarihi: 7/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001355488

pISSN: 2149-3359

Sayı

Yazarlar:

Esra Bilici | Cevdet Uğuz | Korhan Altunbaş

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Van Veterinary Journal Vol.36 Issue 2, pp.110-114

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355488

pISSN:

2149-3359