Immunodetection of insulin-like growth factor binding proteins (IGFBPs) in the sera of different animal species

Romana MASNIKOSA; İvona BARICEVIC; Dragana LAGUNDZIN; Olgica NEDIC

Makale detayları

Immunodetection of insulin-like growth factor binding proteins (IGFBPs) in the sera of different animal species

Yazarlar:

Romana MASNIKOSA | İvona BARICEVIC | Dragana LAGUNDZIN | Olgica NEDIC

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 6, pp.443-452

Belge Tipi:

Makale

Yayın Tarihi:

5/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000767091

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Knowledge regarding the insulin-like growth factor (IGF) system and the methods for the detection and quantitation of IGF binding proteins (IGFBPs) is greater for the human species than for other animal species. This is mainly because antibodies specifically raised against nonhuman IGFBPs are rarely commercially available. We aimed to investigate which of the antibodies directed against human IGFBPs could be used for the detection of IGFBPs present in other species. We used commercially available antibodies, raised against human IGFBP-1 through IGFBP-4, in order to recognize the corresponding IGFBPs in the sera of different animal species: human, bovine, equine, porcine, ovine, guinea pig, rat, and murine. Serum samples were analyzed by SDS-PAGE followed by western blotting, using 12 different polyclonal antibodies and antisera raised against IGFBPs as primary antibodies. Detection of IGFBP-1, -2, -3, and -4 in the sera of animals was achieved to a different extent depending on the primary anti-IGFBP antibodies used. The results of this article may be considered as an initial guide in the designing of experiments in which the detection of animal IGFBPs is required.

 

Knowledge regarding the insulin-like growth factor (IGF) system and the methods for the detection and quantitation of IGF binding proteins (IGFBPs) is greater for the human species than for other animal species. This is mainly because antibodies specifically raised against nonhuman IGFBPs are rarely commercially available. We aimed to investigate which of the antibodies directed against human IGFBPs could be used for the detection of IGFBPs present in other species. We used commercially available antibodies, raised against human IGFBP-1 through IGFBP-4, in order to recognize the corresponding IGFBPs in the sera of different animal species: human, bovine, equine, porcine, ovine, guinea pig, rat, and murine. Serum samples were analyzed by SDS-PAGE followed by western blotting, using 12 different polyclonal antibodies and antisera raised against IGFBPs as primary antibodies. Detection of IGFBP-1, -2, -3, and -4 in the sera of animals was achieved to a different extent depending on the primary anti-IGFBP antibodies used. The results of this article may be considered as an initial guide in the designing of experiments in which the detection of animal IGFBPs is required.

Makale detayları

Immunodetection of insulin-like growth factor binding proteins (IGFBPs) in the sera of different animal species

Yazarlar:

Romana MASNIKOSA | İvona BARICEVIC | Dragana LAGUNDZIN | Olgica NEDIC

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 6, pp.443-452

Belge Tipi:

Makale

Yayın Tarihi:

5/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000767091

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Western blotting

IGFBPs

human

bovine

equine

porcine

ovine

guinea pig

rat

murine

Sayı

Makale detayları

Yazarlar: Romana MASNIKOSA | İvona BARICEVIC | Dragana LAGUNDZIN | Olgica NEDIC

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 6, pp.443-452

Belge Tipi: Makale

Yayın Tarihi: 5/2011

Belge Dili: Türkçe

Document ID (Org No.): 2000767091

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Romana MASNIKOSA | İvona BARICEVIC | Dragana LAGUNDZIN | Olgica NEDIC

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 6, pp.443-452

Belge Tipi:

Makale

Yayın Tarihi:

5/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000767091

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128