Comparison of the Results of Serum Total Protein Concentration Measured by 3 Methods: Preliminary Results

Teodora M. GEORGIEVA; Dimitrinka S. ZAPRYANOVA

Makale detayları

Comparison of the Results of Serum Total Protein Concentration Measured by 3 Methods: Preliminary Results

Yazarlar:

Teodora M. GEORGIEVA | Dimitrinka S. ZAPRYANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2009 Vol.33 Issue 1, pp.67-70

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000767839

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The present study provides the results from a comparative study of the 3 commonly used methods for total protein (TP) measurement. The experiments were carried out with 6 dogs (4-7 year-old, weighing 12.8 ± 1.4 kg). Five blood samples were obtained by saphena venepuncture from all dogs, during the time course of the experimentally induced infection with Staphylococcus intermedius, administered subcutaneously at a dose rate of 5 ml of 1.109 CFU/ml within 14 days. TP concentration was measured by 2 macro protein techniques - biuret method (commonly used) and method of Lowry, and a modified version of biuret method (micro protein technique), suggested by Popov. Serum TP concentration determined by the method of Lowry was significantly (P < 0.001) higher than the ones obtained by standard biuret and Popov´s methods. The mean differences between TP values obtained by standard biuret technique and Lowry´s method and, Lowry´s and Popov´s method were 18.6 g/l and 23.5 g/l, respectively. There was no statistically significant difference between standard biuret method and its modified version suggested by Popov.

 

The present study provides the results from a comparative study of the 3 commonly used methods for total protein (TP) measurement. The experiments were carried out with 6 dogs (4-7 year-old, weighing 12.8 ± 1.4 kg). Five blood samples were obtained by saphena venepuncture from all dogs, during the time course of the experimentally induced infection with Staphylococcus intermedius, administered subcutaneously at a dose rate of 5 ml of 1.109 CFU/ml within 14 days. TP concentration was measured by 2 macro protein techniques - biuret method (commonly used) and method of Lowry, and a modified version of biuret method (micro protein technique), suggested by Popov. Serum TP concentration determined by the method of Lowry was significantly (P < 0.001) higher than the ones obtained by standard biuret and Popov´s methods. The mean differences between TP values obtained by standard biuret technique and Lowry´s method and, Lowry´s and Popov´s method were 18.6 g/l and 23.5 g/l, respectively. There was no statistically significant difference between standard biuret method and its modified version suggested by Popov.

Makale detayları

Comparison of the Results of Serum Total Protein Concentration Measured by 3 Methods: Preliminary Results

Yazarlar:

Teodora M. GEORGIEVA | Dimitrinka S. ZAPRYANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2009 Vol.33 Issue 1, pp.67-70

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000767839

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Total protein concentration

dogs

biuret method

method of Lowry

modification biuret technique by Popov

Sayı

Makale detayları

Comparison of the Results of Serum Total Protein Concentration Measured by 3 Methods: Preliminary Results

Yazarlar: Teodora M. GEORGIEVA | Dimitrinka S. ZAPRYANOVA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2009 Vol.33 Issue 1, pp.67-70

Belge Tipi: Makale

Yayın Tarihi: 12/2008

Belge Dili: Türkçe

Document ID (Org No.): 2000767839

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Total protein concentration

dogs

biuret method

method of Lowry

modification biuret technique by Popov

Sayı

Yazarlar:

Teodora M. GEORGIEVA | Dimitrinka S. ZAPRYANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2009 Vol.33 Issue 1, pp.67-70

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000767839

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128