Investigation of Viral and Bacterial Agents in Samples Taken from Patients with Suspected Upper Respiratory Tract Infection

Murat Karameşe

Makale detayları

Investigation of Viral and Bacterial Agents in Samples Taken from Patients with Suspected Upper Respiratory Tract Infection

Yazarlar:

Murat Karameşe

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.15 Issue 1, pp.58-63

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347650

pISSN:

2146-2631

Anahtar kelimeler:

Özet:

 Aim: Upper respiratory tract infection URTI complaints are the most common causes of admission to outpatient clinics and rapid detection by Multiplex-PCR method contributes to the monitoring and control of infection by easily evaluating the etiology of viral and bacterial agents regionally. The aim of this study was to evaluate the etiology of pathogens with a 24-X viral and bacterial respiratory Multiplex-PCR panel in patients who admitted to XXX University XXX Hospital with URTI symptoms between November 2023 and February 2024. Materials and Methods: The presence of viral and bacterial pathogens was analyzed by Multiplex-PCR method in nasopharyngeal swab samples obtained from 100 patients diagnosed with URTI by physical examination such as allergic rhinitis and acute bronchitis. The respiratory panel was containing 24 different microorganism such as SARS CoV-2, Influenza-AB, Human RhinovirusEnterovirus, Human Metapneumovirus, Respiratory Syncytial Virus AB, Human Parainfluenza Virus-1234, Human Coronavirus 229EOC43NL63HKU1, Human Parechovirus, Adenovirus, Human Bocavirus, Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila and Bordotella pertussis. Results: According to the findings, the highest number of applications was in January. Out of 100 patients, 73 n73 were positive and 27 n27 were negative, the most common viral agent was INF A n33, 26.9 while the most common bacterial agent was S.pneumoniae n19, 15.4. Of 73 positive patients, 38.3 n28 had co-infection, 11 n8 had only bacterial agents and 50.7 n37 had viral agents. Conclusions: In conclusion, URTI agents were rapidly detected by Multiplex-PCR and their incidence was investigated in this study. Finally, it is aimed to prevent possible epidemics, unnecessary antibiotic use, mortality and morbidity, and to contribute to other academic studies with rapid diagnosis and treatment of URTI infections.

 Aim: Upper respiratory tract infection URTI complaints are the most common causes of admission to outpatient clinics and rapid detection by Multiplex-PCR method contributes to the monitoring and control of infection by easily evaluating the etiology of viral and bacterial agents regionally. The aim of this study was to evaluate the etiology of pathogens with a 24-X viral and bacterial respiratory Multiplex-PCR panel in patients who admitted to XXX University XXX Hospital with URTI symptoms between November 2023 and February 2024. Materials and Methods: The presence of viral and bacterial pathogens was analyzed by Multiplex-PCR method in nasopharyngeal swab samples obtained from 100 patients diagnosed with URTI by physical examination such as allergic rhinitis and acute bronchitis. The respiratory panel was containing 24 different microorganism such as SARS CoV-2, Influenza-AB, Human RhinovirusEnterovirus, Human Metapneumovirus, Respiratory Syncytial Virus AB, Human Parainfluenza Virus-1234, Human Coronavirus 229EOC43NL63HKU1, Human Parechovirus, Adenovirus, Human Bocavirus, Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila and Bordotella pertussis. Results: According to the findings, the highest number of applications was in January. Out of 100 patients, 73 n73 were positive and 27 n27 were negative, the most common viral agent was INF A n33, 26.9 while the most common bacterial agent was S.pneumoniae n19, 15.4. Of 73 positive patients, 38.3 n28 had co-infection, 11 n8 had only bacterial agents and 50.7 n37 had viral agents. Conclusions: In conclusion, URTI agents were rapidly detected by Multiplex-PCR and their incidence was investigated in this study. Finally, it is aimed to prevent possible epidemics, unnecessary antibiotic use, mortality and morbidity, and to contribute to other academic studies with rapid diagnosis and treatment of URTI infections.

 Aim: Upper respiratory tract infection URTI complaints are the most common causes of admission to outpatient clinics and rapid detection by Multiplex-PCR method contributes to the monitoring and control of infection by easily evaluating the etiology of viral and bacterial agents regionally. The aim of this study was to evaluate the etiology of pathogens with a 24-X viral and bacterial respiratory Multiplex-PCR panel in patients who admitted to XXX University XXX Hospital with URTI symptoms between November 2023 and February 2024. Materials and Methods: The presence of viral and bacterial pathogens was analyzed by Multiplex-PCR method in nasopharyngeal swab samples obtained from 100 patients diagnosed with URTI by physical examination such as allergic rhinitis and acute bronchitis. The respiratory panel was containing 24 different microorganism such as SARS CoV-2, Influenza-AB, Human RhinovirusEnterovirus, Human Metapneumovirus, Respiratory Syncytial Virus AB, Human Parainfluenza Virus-1234, Human Coronavirus 229EOC43NL63HKU1, Human Parechovirus, Adenovirus, Human Bocavirus, Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila and Bordotella pertussis. Results: According to the findings, the highest number of applications was in January. Out of 100 patients, 73 n73 were positive and 27 n27 were negative, the most common viral agent was INF A n33, 26.9 while the most common bacterial agent was S.pneumoniae n19, 15.4. Of 73 positive patients, 38.3 n28 had co-infection, 11 n8 had only bacterial agents and 50.7 n37 had viral agents. Conclusions: In conclusion, URTI agents were rapidly detected by Multiplex-PCR and their incidence was investigated in this study. Finally, it is aimed to prevent possible epidemics, unnecessary antibiotic use, mortality and morbidity, and to contribute to other academic studies with rapid diagnosis and treatment of URTI infections.

 Aim: Upper respiratory tract infection URTI complaints are the most common causes of admission to outpatient clinics and rapid detection by Multiplex-PCR method contributes to the monitoring and control of infection by easily evaluating the etiology of viral and bacterial agents regionally. The aim of this study was to evaluate the etiology of pathogens with a 24-X viral and bacterial respiratory Multiplex-PCR panel in patients who admitted to XXX University XXX Hospital with URTI symptoms between November 2023 and February 2024. Materials and Methods: The presence of viral and bacterial pathogens was analyzed by Multiplex-PCR method in nasopharyngeal swab samples obtained from 100 patients diagnosed with URTI by physical examination such as allergic rhinitis and acute bronchitis. The respiratory panel was containing 24 different microorganism such as SARS CoV-2, Influenza-AB, Human RhinovirusEnterovirus, Human Metapneumovirus, Respiratory Syncytial Virus AB, Human Parainfluenza Virus-1234, Human Coronavirus 229EOC43NL63HKU1, Human Parechovirus, Adenovirus, Human Bocavirus, Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila and Bordotella pertussis. Results: According to the findings, the highest number of applications was in January. Out of 100 patients, 73 n73 were positive and 27 n27 were negative, the most common viral agent was INF A n33, 26.9 while the most common bacterial agent was S.pneumoniae n19, 15.4. Of 73 positive patients, 38.3 n28 had co-infection, 11 n8 had only bacterial agents and 50.7 n37 had viral agents. Conclusions: In conclusion, URTI agents were rapidly detected by Multiplex-PCR and their incidence was investigated in this study. Finally, it is aimed to prevent possible epidemics, unnecessary antibiotic use, mortality and morbidity, and to contribute to other academic studies with rapid diagnosis and treatment of URTI infections.

Makale detayları

Investigation of Viral and Bacterial Agents in Samples Taken from Patients with Suspected Upper Respiratory Tract Infection

Yazarlar:

Murat Karameşe

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.15 Issue 1, pp.58-63

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347650

pISSN:

2146-2631

URTI

SARS-CoV-2

Influenza

Multiplex PCR

Sayı

Makale detayları

Investigation of Viral and Bacterial Agents in Samples Taken from Patients with Suspected Upper Respiratory Tract Infection

Yazarlar: Murat Karameşe

Yayıncı: Kafkas University

Ana Başlıklar: Klinik Mikrobiyoloji

Dergi: Kafkas Tıp Bilimleri Dergisi Vol.15 Issue 1, pp.58-63

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347650

pISSN: 2146-2631

URTI

SARS-CoV-2

Influenza

Multiplex PCR

Sayı

Yazarlar:

Murat Karameşe

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.15 Issue 1, pp.58-63

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347650

pISSN:

2146-2631