Individual Identification and Assessment of Genetic Diversity Using Microsatellite Markers in Racing Pigeons Raised in Turkiye

Nursen Şentürk; Sebahat Dilara Taşkın; Özden Çobanoğlu; Sena Ardıclı

Makale detayları

Individual Identification and Assessment of Genetic Diversity Using Microsatellite Markers in Racing Pigeons Raised in Turkiye

Yazarlar:

Nursen Şentürk | Sebahat Dilara Taşkın | Özden Çobanoğlu | Sena Ardıclı

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Journal of Research in Veterinary Medicine Vol.43 Issue 1, pp.76-84

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326438

pISSN:

2667-6745

Anahtar kelimeler:

Özet:

 The implementation of a swift and economical molecular genetic approach, ensuring both efficacy and cost-effectiveness and facilitating population certification, is of utmost significance for breeders and the conservation of Turkiyes native pigeon biodiversity. In this study, we aimed to examine the genetic structure of racing pigeons Columba livia domestica raised in Turkiye using a genetic marker panel consisting of eight short tandem repeat STR loci. For this purpose, DNA was isolated from the shed feathers of 216 pigeons. Genomic DNA was amplified using the multiplex allele-specific PCR and subsequent capillary electrophoresis with ABI PRISM 3130XL Genetic Analyzer. Next, PCR products were analyzed in the GeneMapper Software program Applied Biosystems. For parent testing, paternity index PI, combined paternity index CPI, and cumulative probability of paternity CPP were calculated. Furthermore, population genetic diversity was evaluated using heterozygosity He, polymorphism information content PIC, and HardyWeinberg equilibrium HWE testing. Results revealed that the total number of alleles is 81 and the number of alleles per locus varies between 4 and 19. The similarity rate between parent and offspring was calculated as 99.99 and above. Since no pedigree information was given when the samples were analyzed, obtaining this similarity ratio demonstrates the reliability of the panel. He values range from 0.362 to 0.919, and the PIC values range from 0.295 to 0.909. Loci PG-1, PG-2, and PG-3 show significant genetic diversity, with moderate to high PIC values reflecting varied allele frequencies in the population. Consequently, the set of seven STR markers  one sex marker can be applied to identify and confirm parentage on a regular basis, thereby facilitating efficient breeding programs and ensuring genetic diversity conservation. This panel enables efficient pedigree analysis and gender determination, optimizing cost-effectiveness. The methodology presented in this study is ideal for pedigree analysis and breed certification in the Turkish pigeon breeding industry. Consequently, we affirm that the study data carries considerable national importance.

 The implementation of a swift and economical molecular genetic approach, ensuring both efficacy and cost-effectiveness and facilitating population certification, is of utmost significance for breeders and the conservation of Turkiyes native pigeon biodiversity. In this study, we aimed to examine the genetic structure of racing pigeons Columba livia domestica raised in Turkiye using a genetic marker panel consisting of eight short tandem repeat STR loci. For this purpose, DNA was isolated from the shed feathers of 216 pigeons. Genomic DNA was amplified using the multiplex allele-specific PCR and subsequent capillary electrophoresis with ABI PRISM 3130XL Genetic Analyzer. Next, PCR products were analyzed in the GeneMapper Software program Applied Biosystems. For parent testing, paternity index PI, combined paternity index CPI, and cumulative probability of paternity CPP were calculated. Furthermore, population genetic diversity was evaluated using heterozygosity He, polymorphism information content PIC, and HardyWeinberg equilibrium HWE testing. Results revealed that the total number of alleles is 81 and the number of alleles per locus varies between 4 and 19. The similarity rate between parent and offspring was calculated as 99.99 and above. Since no pedigree information was given when the samples were analyzed, obtaining this similarity ratio demonstrates the reliability of the panel. He values range from 0.362 to 0.919, and the PIC values range from 0.295 to 0.909. Loci PG-1, PG-2, and PG-3 show significant genetic diversity, with moderate to high PIC values reflecting varied allele frequencies in the population. Consequently, the set of seven STR markers  one sex marker can be applied to identify and confirm parentage on a regular basis, thereby facilitating efficient breeding programs and ensuring genetic diversity conservation. This panel enables efficient pedigree analysis and gender determination, optimizing cost-effectiveness. The methodology presented in this study is ideal for pedigree analysis and breed certification in the Turkish pigeon breeding industry. Consequently, we affirm that the study data carries considerable national importance.

 The implementation of a swift and economical molecular genetic approach, ensuring both efficacy and cost-effectiveness and facilitating population certification, is of utmost significance for breeders and the conservation of Turkiyes native pigeon biodiversity. In this study, we aimed to examine the genetic structure of racing pigeons Columba livia domestica raised in Turkiye using a genetic marker panel consisting of eight short tandem repeat STR loci. For this purpose, DNA was isolated from the shed feathers of 216 pigeons. Genomic DNA was amplified using the multiplex allele-specific PCR and subsequent capillary electrophoresis with ABI PRISM 3130XL Genetic Analyzer. Next, PCR products were analyzed in the GeneMapper Software program Applied Biosystems. For parent testing, paternity index PI, combined paternity index CPI, and cumulative probability of paternity CPP were calculated. Furthermore, population genetic diversity was evaluated using heterozygosity He, polymorphism information content PIC, and HardyWeinberg equilibrium HWE testing. Results revealed that the total number of alleles is 81 and the number of alleles per locus varies between 4 and 19. The similarity rate between parent and offspring was calculated as 99.99 and above. Since no pedigree information was given when the samples were analyzed, obtaining this similarity ratio demonstrates the reliability of the panel. He values range from 0.362 to 0.919, and the PIC values range from 0.295 to 0.909. Loci PG-1, PG-2, and PG-3 show significant genetic diversity, with moderate to high PIC values reflecting varied allele frequencies in the population. Consequently, the set of seven STR markers  one sex marker can be applied to identify and confirm parentage on a regular basis, thereby facilitating efficient breeding programs and ensuring genetic diversity conservation. This panel enables efficient pedigree analysis and gender determination, optimizing cost-effectiveness. The methodology presented in this study is ideal for pedigree analysis and breed certification in the Turkish pigeon breeding industry. Consequently, we affirm that the study data carries considerable national importance.

 The implementation of a swift and economical molecular genetic approach, ensuring both efficacy and cost-effectiveness and facilitating population certification, is of utmost significance for breeders and the conservation of Turkiyes native pigeon biodiversity. In this study, we aimed to examine the genetic structure of racing pigeons Columba livia domestica raised in Turkiye using a genetic marker panel consisting of eight short tandem repeat STR loci. For this purpose, DNA was isolated from the shed feathers of 216 pigeons. Genomic DNA was amplified using the multiplex allele-specific PCR and subsequent capillary electrophoresis with ABI PRISM 3130XL Genetic Analyzer. Next, PCR products were analyzed in the GeneMapper Software program Applied Biosystems. For parent testing, paternity index PI, combined paternity index CPI, and cumulative probability of paternity CPP were calculated. Furthermore, population genetic diversity was evaluated using heterozygosity He, polymorphism information content PIC, and HardyWeinberg equilibrium HWE testing. Results revealed that the total number of alleles is 81 and the number of alleles per locus varies between 4 and 19. The similarity rate between parent and offspring was calculated as 99.99 and above. Since no pedigree information was given when the samples were analyzed, obtaining this similarity ratio demonstrates the reliability of the panel. He values range from 0.362 to 0.919, and the PIC values range from 0.295 to 0.909. Loci PG-1, PG-2, and PG-3 show significant genetic diversity, with moderate to high PIC values reflecting varied allele frequencies in the population. Consequently, the set of seven STR markers  one sex marker can be applied to identify and confirm parentage on a regular basis, thereby facilitating efficient breeding programs and ensuring genetic diversity conservation. This panel enables efficient pedigree analysis and gender determination, optimizing cost-effectiveness. The methodology presented in this study is ideal for pedigree analysis and breed certification in the Turkish pigeon breeding industry. Consequently, we affirm that the study data carries considerable national importance.

Makale detayları

Individual Identification and Assessment of Genetic Diversity Using Microsatellite Markers in Racing Pigeons Raised in Turkiye

Yazarlar:

Nursen Şentürk | Sebahat Dilara Taşkın | Özden Çobanoğlu | Sena Ardıclı

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Journal of Research in Veterinary Medicine Vol.43 Issue 1, pp.76-84

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326438

pISSN:

2667-6745

parentage testing

microsatellite markers

genetic diversity

population structure

Columba livia domestica

Sayı

Makale detayları

Individual Identification and Assessment of Genetic Diversity Using Microsatellite Markers in Racing Pigeons Raised in Turkiye

Yazarlar: Nursen Şentürk | Sebahat Dilara Taşkın | Özden Çobanoğlu | Sena Ardıclı

Yayıncı: Bursa Uludağ University

Ana Başlıklar: Veterinary Sciences (Other)

Dergi: Journal of Research in Veterinary Medicine Vol.43 Issue 1, pp.76-84

Belge Tipi: Makale

Yayın Tarihi: 2/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326438

pISSN: 2667-6745

parentage testing

microsatellite markers

genetic diversity

population structure

Columba livia domestica

Sayı

Yazarlar:

Nursen Şentürk | Sebahat Dilara Taşkın | Özden Çobanoğlu | Sena Ardıclı

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Journal of Research in Veterinary Medicine Vol.43 Issue 1, pp.76-84

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326438

pISSN:

2667-6745