Effects of Rhizobacteria and Auxin Treatments on Root Development of Black Mulberry (Morus nigra L.) Cuttings

Onur Sefa Alkaç; Hakan Karadağ; Çetin Çekiç

Makale detayları

Effects of Rhizobacteria and Auxin Treatments on Root Development of Black Mulberry (Morus nigra L.) Cuttings

Yazarlar:

Onur Sefa Alkaç | Hakan Karadağ | Çetin Çekiç

Yayıncı:

Mevlüt AKÇURA

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 1, pp.8-14

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001283213

pISSN:

2148-3647

Anahtar kelimeler:

Özet:

 Growth regulators such as IBA are generally used to promote root formation of black mulberry cuttings. However, today, studies on the research of alternative materials and methods to synthetic chemicals in the plant production process within the scope of good agricultural practices are gaining momentum. The common alternative application is rhizobacteria PGPR, which is increasingly used today. In this study, the effects of two different bacterial isolates Bacillus cereus ZE-7 and Pseudomonas putida ZE-12 that can be alternatives to growth regulators on the rooting of black mulberry wood cuttings were investigated. For this purpose, two bacterial isolates were applied alone or together with 6000 ppm auxin Indole-3-butyric acid-IBA to wood cuttings taken at the end of December from the black mulberry growing glasshose of Tokat Gaziosmanpaşa University Research and Application Center. After the cuttings were kept in IBA solution for 10 seconds and then in bacterial suspensions for 30 minutes, they were planted on perlite rooting medium with underfloor heating system. The parameters of root number piecestem, root length cm, root diameter mm, decay rate, rooting rate  and callus rate  were investigated in cuttings that remained in rooting medium for 90 days. The highest rooting rate was obtained from 6000 ppm IBA  0.3 Abs bacteria application and 6000 ppm IBA  1.2 Abs bacteria application 71.11 . The rooting rate increased by 28 with the combination of bacteria compared to the control. Bacteria applications generally had positive effects on root number, decay rate, rooting rate and callus rate, but did not have a positive effect on root length and root diameter parameters. As a result, 6000 ppm is applied as standard IBA concentration in black mulberry cuttings, but this is not enough. With the results obtained, it is seen that 28 increase can be achieved compared to the standard application by applying bacteria at 0.3 Abs concentration in addition to this concentration. In addition, thanks to the advantages of bacterial application such as being sustainable, easy to prepare and applicable, it is recommended for rooting studies planned in the future.

 Growth regulators such as IBA are generally used to promote root formation of black mulberry cuttings. However, today, studies on the research of alternative materials and methods to synthetic chemicals in the plant production process within the scope of good agricultural practices are gaining momentum. The common alternative application is rhizobacteria PGPR, which is increasingly used today. In this study, the effects of two different bacterial isolates Bacillus cereus ZE-7 and Pseudomonas putida ZE-12 that can be alternatives to growth regulators on the rooting of black mulberry wood cuttings were investigated. For this purpose, two bacterial isolates were applied alone or together with 6000 ppm auxin Indole-3-butyric acid-IBA to wood cuttings taken at the end of December from the black mulberry growing glasshose of Tokat Gaziosmanpaşa University Research and Application Center. After the cuttings were kept in IBA solution for 10 seconds and then in bacterial suspensions for 30 minutes, they were planted on perlite rooting medium with underfloor heating system. The parameters of root number piecestem, root length cm, root diameter mm, decay rate, rooting rate  and callus rate  were investigated in cuttings that remained in rooting medium for 90 days. The highest rooting rate was obtained from 6000 ppm IBA  0.3 Abs bacteria application and 6000 ppm IBA  1.2 Abs bacteria application 71.11 . The rooting rate increased by 28 with the combination of bacteria compared to the control. Bacteria applications generally had positive effects on root number, decay rate, rooting rate and callus rate, but did not have a positive effect on root length and root diameter parameters. As a result, 6000 ppm is applied as standard IBA concentration in black mulberry cuttings, but this is not enough. With the results obtained, it is seen that 28 increase can be achieved compared to the standard application by applying bacteria at 0.3 Abs concentration in addition to this concentration. In addition, thanks to the advantages of bacterial application such as being sustainable, easy to prepare and applicable, it is recommended for rooting studies planned in the future.

 Growth regulators such as IBA are generally used to promote root formation of black mulberry cuttings. However, today, studies on the research of alternative materials and methods to synthetic chemicals in the plant production process within the scope of good agricultural practices are gaining momentum. The common alternative application is rhizobacteria PGPR, which is increasingly used today. In this study, the effects of two different bacterial isolates Bacillus cereus ZE-7 and Pseudomonas putida ZE-12 that can be alternatives to growth regulators on the rooting of black mulberry wood cuttings were investigated. For this purpose, two bacterial isolates were applied alone or together with 6000 ppm auxin Indole-3-butyric acid-IBA to wood cuttings taken at the end of December from the black mulberry growing glasshose of Tokat Gaziosmanpaşa University Research and Application Center. After the cuttings were kept in IBA solution for 10 seconds and then in bacterial suspensions for 30 minutes, they were planted on perlite rooting medium with underfloor heating system. The parameters of root number piecestem, root length cm, root diameter mm, decay rate, rooting rate  and callus rate  were investigated in cuttings that remained in rooting medium for 90 days. The highest rooting rate was obtained from 6000 ppm IBA  0.3 Abs bacteria application and 6000 ppm IBA  1.2 Abs bacteria application 71.11 . The rooting rate increased by 28 with the combination of bacteria compared to the control. Bacteria applications generally had positive effects on root number, decay rate, rooting rate and callus rate, but did not have a positive effect on root length and root diameter parameters. As a result, 6000 ppm is applied as standard IBA concentration in black mulberry cuttings, but this is not enough. With the results obtained, it is seen that 28 increase can be achieved compared to the standard application by applying bacteria at 0.3 Abs concentration in addition to this concentration. In addition, thanks to the advantages of bacterial application such as being sustainable, easy to prepare and applicable, it is recommended for rooting studies planned in the future.

 Karadut çeliklerinin kök oluşumunu teşvik etmek amacıyla genellikle IBA gibi büyüme düzenleyiciler kullanılmaktadır. Ancak, günümüzde sürdürülebilir tarım uygulamaları kapsamında, bitkisel üretim sürecinde sentetik kimyasallara alternatif yeni materyallerin ve yöntemlerin araştırma çalışmaları giderek hız kazanmaktadır. Bu tür alternatif uygulamaların başında ise, günümüzde kullanımı gittikçe yaygınlaşan rizobakteriler PGPR yer almaktadır. Bu çalışmada da büyüme düzenleyicilerine alternatif olabilecek iki farklı bakteri izolatının Bacillus cereus ZE-7 ve Pseudomonas putida ZE-12 karadut odun çeliklerinin köklenmesi üzerine etkisi araştırılmıştır. Bu amaçla, aralık ayı sonunda Tokat Gaziosmanpaşa Üniversitesi Araştırma ve Uygulama Merkezine ait karadut yetiştiriciliği yapılan seradan temin edilen odun çeliklerine bakteri izolatları tek başına ve 6000 ppm oksin Indole-3-butyric acid-IBA ile birlikte uygulanmıştır. Çelikler 10 saniye IBA çözeltisinde ardından 30 dakika bakteri süspansiyonlarında bekletildikten sonra sisleme ve alttan ısıtma sistemi olan köklendirme masasına, tamamı perlit olan ortama dikilmiştir. 90 gün köklenme ortamında kalan çeliklerde kök sayısı adetçelik, kök uzunluğu cm, kök çapı mm, çürüme oranı, köklenme oranı  ve kallüslenme oranı  parametreleri incelenmiştir. En yüksek köklenme oranı, 6000 ppm IBA  0.3 Abs bakteri uygulaması ve 6000 ppm IBA  1.2 Abs bakteri uygulamasından 71.11 elde edilmiştir. Kontrole kıyasla köklenme oranı bakterinin kombinesi ile 28 oranında artmıştır. Bakteri uygulamaları genel olarak, kök sayısı, çürüme oranı, köklenme oranı ve kallüslenme oranına olumlu etkilerde bulunurken, kök uzunluğu ve kök çapı parametrelerinde ise artı bir etkisi olmamıştır. Sonuç olarak karadut çeliklerinde standart IBA konsantrasyonu olarak 6000 ppm uygulanmakta ancak bu yeterli gelmemektedir. Elde edilen sonuçlarla bu konsantrasyona ek olarak 0.3 Abs konsantrasyonunda bakteri uygulaması yapılarak standart uygulamaya kıyasla 28 artış sağlanabileceği sonuçlarda yer almaktadır. Ayrıca bakteri uygulamasının sürdürülebilir, kolay hazırlanabilir ve uygulanabilir olması gibi avantajları sayesinde ileride yapılması planlanan köklendirme çalışmalarında önerilmektedir.

Makale detayları

Effects of Rhizobacteria and Auxin Treatments on Root Development of Black Mulberry (Morus nigra L.) Cuttings

Yazarlar:

Onur Sefa Alkaç | Hakan Karadağ | Çetin Çekiç

Yayıncı:

Mevlüt AKÇURA

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 1, pp.8-14

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001283213

pISSN:

2148-3647

Bacillus cereus

Pseudomonas putida

IBA

çelik

köklendirme

cutting

rooting.

Sayı

Makale detayları

Yazarlar: Onur Sefa Alkaç | Hakan Karadağ | Çetin Çekiç

Yayıncı: Mevlüt AKÇURA

Dergi: Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 1, pp.8-14

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001283213

pISSN: 2148-3647

Sayı

Yazarlar:

Onur Sefa Alkaç | Hakan Karadağ | Çetin Çekiç

Yayıncı:

Mevlüt AKÇURA

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 1, pp.8-14

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001283213

pISSN:

2148-3647