Lack of Pseudouridine Synthase 7 Enzyme Sensitizes Yeast Cells to DNA-Damaging Agents

Mehmet Tardu

Makale detayları

Lack of Pseudouridine Synthase 7 Enzyme Sensitizes Yeast Cells to DNA-Damaging Agents

Yazarlar:

Mehmet Tardu

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Mikrobiyal Genetik

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.8 Issue 4, pp.1613-1623

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354228

pISSN:

2687-3729

Anahtar kelimeler:

Özet:

 RNA modifications are essential in reshaping the chemistry of RNA molecules, influencing their structure, function, and stability. Up to now, more than 160 different types of modifications have been discovered among different RNA species. One prominent modification is the enzymatic transformation of uridine U into pseudouridine Ψ, a process facilitated by pseudouridine synthase Pus enzymes, which occurs in both coding mRNA and non-coding ncRNA RNA species. Among the enzymes responsible for this transformation, Pus7 stands out due to its association with certain developmental and physical conditions. Research links Pus7 to intellectual disabilities, delayed speech development, microcephaly, and short stature. Despite its association with these disorders, the exact biological role and mechanism of Pus7 remain poorly understood, leaving room for further investigation. In this study, we aimed to understand the role of Pus7 in Saccharomyces cerevisiae cells under DNA damage stress. To achieve this, wild-type and pus7Δ cells were subjected to varying concentrations of DNA-damaging agents, including ultraviolet UV light and the chemical methyl methanesulfonate MMS. Survival curves and spot plating assay results demonstrated that pus7Δ cells exhibit growth defects when exposed to 2 mM MMS or 15 Jm² UV light. These findings indicate that the absence of Pus7 enzyme renders yeast cells sensitive to DNA-damaging agents. Further research is necessary to investigate the role of Pus7 under DNA damage stress, which contributes to genomic instability - a hallmark of many cancers.

 RNA modifications are essential in reshaping the chemistry of RNA molecules, influencing their structure, function, and stability. Up to now, more than 160 different types of modifications have been discovered among different RNA species. One prominent modification is the enzymatic transformation of uridine U into pseudouridine Ψ, a process facilitated by pseudouridine synthase Pus enzymes, which occurs in both coding mRNA and non-coding ncRNA RNA species. Among the enzymes responsible for this transformation, Pus7 stands out due to its association with certain developmental and physical conditions. Research links Pus7 to intellectual disabilities, delayed speech development, microcephaly, and short stature. Despite its association with these disorders, the exact biological role and mechanism of Pus7 remain poorly understood, leaving room for further investigation. In this study, we aimed to understand the role of Pus7 in Saccharomyces cerevisiae cells under DNA damage stress. To achieve this, wild-type and pus7Δ cells were subjected to varying concentrations of DNA-damaging agents, including ultraviolet UV light and the chemical methyl methanesulfonate MMS. Survival curves and spot plating assay results demonstrated that pus7Δ cells exhibit growth defects when exposed to 2 mM MMS or 15 Jm² UV light. These findings indicate that the absence of Pus7 enzyme renders yeast cells sensitive to DNA-damaging agents. Further research is necessary to investigate the role of Pus7 under DNA damage stress, which contributes to genomic instability - a hallmark of many cancers.

 RNA modifications are essential in reshaping the chemistry of RNA molecules, influencing their structure, function, and stability. Up to now, more than 160 different types of modifications have been discovered among different RNA species. One prominent modification is the enzymatic transformation of uridine U into pseudouridine Ψ, a process facilitated by pseudouridine synthase Pus enzymes, which occurs in both coding mRNA and non-coding ncRNA RNA species. Among the enzymes responsible for this transformation, Pus7 stands out due to its association with certain developmental and physical conditions. Research links Pus7 to intellectual disabilities, delayed speech development, microcephaly, and short stature. Despite its association with these disorders, the exact biological role and mechanism of Pus7 remain poorly understood, leaving room for further investigation. In this study, we aimed to understand the role of Pus7 in Saccharomyces cerevisiae cells under DNA damage stress. To achieve this, wild-type and pus7Δ cells were subjected to varying concentrations of DNA-damaging agents, including ultraviolet UV light and the chemical methyl methanesulfonate MMS. Survival curves and spot plating assay results demonstrated that pus7Δ cells exhibit growth defects when exposed to 2 mM MMS or 15 Jm² UV light. These findings indicate that the absence of Pus7 enzyme renders yeast cells sensitive to DNA-damaging agents. Further research is necessary to investigate the role of Pus7 under DNA damage stress, which contributes to genomic instability - a hallmark of many cancers.

 RNA modifikasyonları, RNA moleküllerinin kimyasını değiştirmede önemli bir rol oynayarak onların yapılarını, işlevselliklerini ve kararlılıklarını etkiler. Bugüne kadar yapılan çalışmalarda çeşitli RNA türleri üzerinde 160tan fazla farklı modifikasyon tipi tanımlanmıştır. Bu modifikasyonlardan biri, uridin U molekülünün psödouridin Ψ formuna enzimatik dönüşümüdür ve bu süreç, psödouridin sentaz Pus enzimleri tarafından gerçekleştirilir. Bu modifikasyon, hem kodlama yapan RNA mRNA hem de kodlama yapmayan RNA ncRNA türlerinde bulunur. Bu dönüşümden sorumlu enzimler arasında, Pus7 belirli gelişimsel ve fiziksel durumlarla ilişkilendirilmesi nedeniyle öne çıkmaktadır. Araştırmalar, Pus7nin zihinsel yetersizlikler, gecikmiş konuşma gelişimi, mikrosefali ve kısa boy gibi durumlarla bağlantılı olduğunu göstermektedir. Bu rahatsızlıklarla ilişkisine rağmen, Pus7nin tam biyolojik rolü ve mekanizması yeterince anlaşılmış değildir ve bu durum daha fazla araştırmaya olanak tanımaktadır. Bu çalışmada, Saccharomyces cerevisiae hücrelerinde Pus7nin DNA hasar stresine karşı rolünü anlamayı amaçladık. Bu amaçla, yabanıl tip ve pus7Δ hücreleri, ultraviyole UV ışığı ve metil metansülfonat MMS gibi DNA hasarına neden olan ajanların farklı konsantrasyonlarına maruz bırakıldı. Sağ kalım eğri analizleri ve okta ekim sonuçları, pus7Δ hücrelerinin 2 mM MMS ve 15 jm² UV ışığı varlığında büyüme kusurları sergilediğini gösterdi. Bu sonuçlar, Pus7 enziminin yokluğunun maya hücrelerini DNA hasarına neden olan ajanlara duyarlı hale getirdiğini ortaya koymaktadır. Sonuçlar bize, birçok kanser türünün ayırt edici özelliği olan genomik dengesizliğe katkıda bulunan DNA hasarı stresi altında Pus7nin rolünü araştırmak için acilen kapsamlı araştırmalara ihtiyaç duyulduğunu göstermektedir.

Makale detayları

Lack of Pseudouridine Synthase 7 Enzyme Sensitizes Yeast Cells to DNA-Damaging Agents

Yazarlar:

Mehmet Tardu

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Mikrobiyal Genetik

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.8 Issue 4, pp.1613-1623

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354228

pISSN:

2687-3729

Saccharomyces cerevisiae

Pseudouridine synthase

Pus7

Methyl methanesulfonate

Ultraviolet light

DNA damageSaccharomyces cerevisiae

Psödouridin sentaz

Pus7

Metil metansülfonat

Ultraviyole ışık

DNA hasarı

Sayı

Makale detayları

Yazarlar: Mehmet Tardu

Yayıncı: Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar: Mikrobiyal Genetik

Dergi: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.8 Issue 4, pp.1613-1623

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001354228

pISSN: 2687-3729

Sayı

Yazarlar:

Mehmet Tardu

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Mikrobiyal Genetik

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.8 Issue 4, pp.1613-1623

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354228

pISSN:

2687-3729