Natural hepatoprotectives earthworm extract protein & goat milk in-vitro model rat primary hepatocytes exposed to carbon tetrachloride revealed toxicity and oxidative stress

Avishek Mandal

Makale detayları

Natural hepatoprotectives earthworm extract protein & goat milk in-vitro model rat primary hepatocytes exposed to carbon tetrachloride revealed toxicity and oxidative stress

Yazarlar:

Avishek Mandal

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Delivery Technologies

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.258-268

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345997

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 The assessment of bioactive ingredients with potential hepatoprotective properties focuses primarily on in-vitro bioassays. This study aimed to assess the effectiveness of pre-treating primary hepatocyte cells with hepatoprotective earthworm extract to prevent damage from carbon tetrachloride CCl4. Cell viability, aspartate aminotransferase AST, alanine aminotransferase ALT, and lipid peroxidation or malondialdehyde MDA levels activities, as well as hepatoprotective activity of Earthworm extract protein powder EEP different concentration were 25, 50, and 100 μgml, Goat milk powder GP and Silymarin SLM 100 ugml, and CCl4 were assessed in-vitro assay on the laboratory model rat primary hepatocyte culture. The selection of the CCl4 minimum lethal dosage might occasionally make examining liver damage more time consuming. Therefore, we examine CCl4 effectiveness at low to high doses in primary rat hepatocytes. Using primary hepatocyte as a toxicity model, we explored the cellular toxicity and oxidative stress related to CCl4 exposure. Our findings imply that the CCl4-induced cytotoxicity is fundamentally influenced by oxidative stress. The MTT assay results exhibited that CCl4 lower to higher doses decreased the viability of primary hepatocytes within six hours of treatment and hepatoprotective activity of EEP was identified. The lipid peroxidation assay revealed that MDA levels were inhibited by EEP and GP treatment in primary hepatocytes. Our results also showed EEP and GP treatment significantly inhibited AST, ALT, inflammatory marker Tnf-α and MDA level increase due to inducing CCl4 in in-vitro rat primary hepatocytes. According to the studies, EEP and GP function as an efficient hepatoprotective agent and prevent CCl4 treatment-induced primary hepatocyte toxicity.

 The assessment of bioactive ingredients with potential hepatoprotective properties focuses primarily on in-vitro bioassays. This study aimed to assess the effectiveness of pre-treating primary hepatocyte cells with hepatoprotective earthworm extract to prevent damage from carbon tetrachloride CCl4. Cell viability, aspartate aminotransferase AST, alanine aminotransferase ALT, and lipid peroxidation or malondialdehyde MDA levels activities, as well as hepatoprotective activity of Earthworm extract protein powder EEP different concentration were 25, 50, and 100 μgml, Goat milk powder GP and Silymarin SLM 100 ugml, and CCl4 were assessed in-vitro assay on the laboratory model rat primary hepatocyte culture. The selection of the CCl4 minimum lethal dosage might occasionally make examining liver damage more time consuming. Therefore, we examine CCl4 effectiveness at low to high doses in primary rat hepatocytes. Using primary hepatocyte as a toxicity model, we explored the cellular toxicity and oxidative stress related to CCl4 exposure. Our findings imply that the CCl4-induced cytotoxicity is fundamentally influenced by oxidative stress. The MTT assay results exhibited that CCl4 lower to higher doses decreased the viability of primary hepatocytes within six hours of treatment and hepatoprotective activity of EEP was identified. The lipid peroxidation assay revealed that MDA levels were inhibited by EEP and GP treatment in primary hepatocytes. Our results also showed EEP and GP treatment significantly inhibited AST, ALT, inflammatory marker Tnf-α and MDA level increase due to inducing CCl4 in in-vitro rat primary hepatocytes. According to the studies, EEP and GP function as an efficient hepatoprotective agent and prevent CCl4 treatment-induced primary hepatocyte toxicity.

 The assessment of bioactive ingredients with potential hepatoprotective properties focuses primarily on in-vitro bioassays. This study aimed to assess the effectiveness of pre-treating primary hepatocyte cells with hepatoprotective earthworm extract to prevent damage from carbon tetrachloride CCl4. Cell viability, aspartate aminotransferase AST, alanine aminotransferase ALT, and lipid peroxidation or malondialdehyde MDA levels activities, as well as hepatoprotective activity of Earthworm extract protein powder EEP different concentration were 25, 50, and 100 μgml, Goat milk powder GP and Silymarin SLM 100 ugml, and CCl4 were assessed in-vitro assay on the laboratory model rat primary hepatocyte culture. The selection of the CCl4 minimum lethal dosage might occasionally make examining liver damage more time consuming. Therefore, we examine CCl4 effectiveness at low to high doses in primary rat hepatocytes. Using primary hepatocyte as a toxicity model, we explored the cellular toxicity and oxidative stress related to CCl4 exposure. Our findings imply that the CCl4-induced cytotoxicity is fundamentally influenced by oxidative stress. The MTT assay results exhibited that CCl4 lower to higher doses decreased the viability of primary hepatocytes within six hours of treatment and hepatoprotective activity of EEP was identified. The lipid peroxidation assay revealed that MDA levels were inhibited by EEP and GP treatment in primary hepatocytes. Our results also showed EEP and GP treatment significantly inhibited AST, ALT, inflammatory marker Tnf-α and MDA level increase due to inducing CCl4 in in-vitro rat primary hepatocytes. According to the studies, EEP and GP function as an efficient hepatoprotective agent and prevent CCl4 treatment-induced primary hepatocyte toxicity.

 The assessment of bioactive ingredients with potential hepatoprotective properties focuses primarily on in-vitro bioassays. This study aimed to assess the effectiveness of pre-treating primary hepatocyte cells with hepatoprotective earthworm extract to prevent damage from carbon tetrachloride CCl4. Cell viability, aspartate aminotransferase AST, alanine aminotransferase ALT, and lipid peroxidation or malondialdehyde MDA levels activities, as well as hepatoprotective activity of Earthworm extract protein powder EEP different concentration were 25, 50, and 100 μgml, Goat milk powder GP and Silymarin SLM 100 ugml, and CCl4 were assessed in-vitro assay on the laboratory model rat primary hepatocyte culture. The selection of the CCl4 minimum lethal dosage might occasionally make examining liver damage more time consuming. Therefore, we examine CCl4 effectiveness at low to high doses in primary rat hepatocytes. Using primary hepatocyte as a toxicity model, we explored the cellular toxicity and oxidative stress related to CCl4 exposure. Our findings imply that the CCl4-induced cytotoxicity is fundamentally influenced by oxidative stress. The MTT assay results exhibited that CCl4 lower to higher doses decreased the viability of primary hepatocytes within six hours of treatment and hepatoprotective activity of EEP was identified. The lipid peroxidation assay revealed that MDA levels were inhibited by EEP and GP treatment in primary hepatocytes. Our results also showed EEP and GP treatment significantly inhibited AST, ALT, inflammatory marker Tnf-α and MDA level increase due to inducing CCl4 in in-vitro rat primary hepatocytes. According to the studies, EEP and GP function as an efficient hepatoprotective agent and prevent CCl4 treatment-induced primary hepatocyte toxicity.

Makale detayları

Natural hepatoprotectives earthworm extract protein & goat milk in-vitro model rat primary hepatocytes exposed to carbon tetrachloride revealed toxicity and oxidative stress

Yazarlar:

Avishek Mandal

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Delivery Technologies

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.258-268

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345997

pISSN:

2630-6344

Hepatoprotective

Primary hepatocyte

Earthworm extract protein

Goatmilk

Silymarin

Sayı

Makale detayları

Natural hepatoprotectives earthworm extract protein & goat milk in-vitro model rat primary hepatocytes exposed to carbon tetrachloride revealed toxicity and oxidative stress

Yazarlar: Avishek Mandal

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Delivery Technologies

Dergi: Journal of Research in Pharmacy Vol.28 Issue 1, pp.258-268

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001345997

pISSN: 2630-6344

Hepatoprotective

Primary hepatocyte

Earthworm extract protein

Goatmilk

Silymarin

Sayı

Yazarlar:

Avishek Mandal

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Delivery Technologies

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.258-268

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345997

pISSN:

2630-6344