Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells

Brown alga Sargassum muticum inhibits Proinflammatory

Makale detayları

Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells

Yazarlar:

Brown alga Sargassum muticum inhibits Proinflammatory

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2010 Vol.34 Issue 1, pp.25-34

Belge Tipi:

Makale

Yayın Tarihi:

12/2009

Belge Dili:

İngilizce

Document ID (Org No.):

2000813110

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH2Cl2, EtOAc, BuOH, and water. The results indicate that the CH2Cl2 fraction of S. muticum extract was an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects of S. muticum fractions on other cytokines, we also measured IL-1β and IL-6 mRNA expression by RT-PCR in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH2Cl2 fraction of S. muticum decreased the expression of IL-1β and IL-6 mRNA in a dose-dependent manner. Based on these results, we suggest that S. muticum extracts may be considered possible anti-inflammatory candidates for human health.

 

The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH2Cl2, EtOAc, BuOH, and water. The results indicate that the CH2Cl2 fraction of S. muticum extract was an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects of S. muticum fractions on other cytokines, we also measured IL-1β and IL-6 mRNA expression by RT-PCR in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH2Cl2 fraction of S. muticum decreased the expression of IL-1β and IL-6 mRNA in a dose-dependent manner. Based on these results, we suggest that S. muticum extracts may be considered possible anti-inflammatory candidates for human health.

Makale detayları

Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells

Yazarlar:

Brown alga Sargassum muticum inhibits Proinflammatory

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2010 Vol.34 Issue 1, pp.25-34

Belge Tipi:

Makale

Yayın Tarihi:

12/2009

Belge Dili:

İngilizce

Document ID (Org No.):

2000813110

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Algae

extract

inflammation

Sargassum muticum

Sayı

Makale detayları

Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells

Yazarlar: Brown alga Sargassum muticum inhibits Proinflammatory

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2010 Vol.34 Issue 1, pp.25-34

Belge Tipi: Makale

Yayın Tarihi: 12/2009

Belge Dili: İngilizce

Document ID (Org No.): 2000813110

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Algae

extract

inflammation

Sargassum muticum

Sayı

Yazarlar:

Brown alga Sargassum muticum inhibits Proinflammatory

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2010 Vol.34 Issue 1, pp.25-34

Belge Tipi:

Makale

Yayın Tarihi:

12/2009

Belge Dili:

İngilizce

Document ID (Org No.):

2000813110

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152