Isolation and Characterization of Cefotaxime and Ciprofloxacin Co-Resistant Escherichia coli in Retail Chicken Carcasses

Özkan Aslantaş; Ahmet Murat Korkut; Mücella Bayırlı

Makale detayları

Isolation and Characterization of Cefotaxime and Ciprofloxacin Co-Resistant Escherichia coli in Retail Chicken Carcasses

Yazarlar:

Özkan Aslantaş | Ahmet Murat Korkut | Mücella Bayırlı

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Mikrobiyolojisi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 2, pp.228-233

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304650

pISSN:

2146-7188

Anahtar kelimeler:

Özet:

 Transmission of antimicrobial-resistant bacteria to humans through the food chain is of great importance for public health. In this study, it was aimed to isolate and characterize the cefotaxime and ciprofloxacin-resistant Escherichia coli in retail chicken meat samples sold in Hatay. The isolates were subjected to phylogenetic group typing and antimicrobial susceptibility testing. The genetic relatedness of the isolates was determined using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction ERIC-PCR technique. The isolates were also screened for the presence of both antimicrobial and plasmid-mediated quinolone resistance PMQR genes by PCR. Cefotaxime and ciprofloxacin co-resistant E. coli isolates with diverse genetic origins were recovered in 42.3 2252 of retail chicken carcasses. The E. coli isolates belonged to the phylogenetic group D2 were dominant 40.9, 922, followed by B1 27.3, 622, B23 18.2, 422, and A1 13.6, 322, respectively. Based on dendrogram analysis, the ERIC-PCR method differentiated the isolates into 10 clusters I-X. The multidrug resistance MDR was observed in 81.8 1822 of the isolates. PMQR determinants were not identified in any isolates tested. Molecular analysis revealed one or more β-lactamase-encoding genes in all isolates as a single or in combination: blaCTX-M-blaTEM n5, blaCMY-2 n5, blaCTX-M n5, blaCMY-2-blaSHV n3, blaCMY-2-blaTEM n3, and blaCTX-M-blaCMY-2 n1. This study highlights that retail chicken meat is an important reservoir of cefotaxime and ciprofloxacin co-resistant E. coli isolates. It is necessary to evaluate their contribution to the community and hospital infections.

 Transmission of antimicrobial-resistant bacteria to humans through the food chain is of great importance for public health. In this study, it was aimed to isolate and characterize the cefotaxime and ciprofloxacin-resistant Escherichia coli in retail chicken meat samples sold in Hatay. The isolates were subjected to phylogenetic group typing and antimicrobial susceptibility testing. The genetic relatedness of the isolates was determined using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction ERIC-PCR technique. The isolates were also screened for the presence of both antimicrobial and plasmid-mediated quinolone resistance PMQR genes by PCR. Cefotaxime and ciprofloxacin co-resistant E. coli isolates with diverse genetic origins were recovered in 42.3 2252 of retail chicken carcasses. The E. coli isolates belonged to the phylogenetic group D2 were dominant 40.9, 922, followed by B1 27.3, 622, B23 18.2, 422, and A1 13.6, 322, respectively. Based on dendrogram analysis, the ERIC-PCR method differentiated the isolates into 10 clusters I-X. The multidrug resistance MDR was observed in 81.8 1822 of the isolates. PMQR determinants were not identified in any isolates tested. Molecular analysis revealed one or more β-lactamase-encoding genes in all isolates as a single or in combination: blaCTX-M-blaTEM n5, blaCMY-2 n5, blaCTX-M n5, blaCMY-2-blaSHV n3, blaCMY-2-blaTEM n3, and blaCTX-M-blaCMY-2 n1. This study highlights that retail chicken meat is an important reservoir of cefotaxime and ciprofloxacin co-resistant E. coli isolates. It is necessary to evaluate their contribution to the community and hospital infections.

 Transmission of antimicrobial-resistant bacteria to humans through the food chain is of great importance for public health. In this study, it was aimed to isolate and characterize the cefotaxime and ciprofloxacin-resistant Escherichia coli in retail chicken meat samples sold in Hatay. The isolates were subjected to phylogenetic group typing and antimicrobial susceptibility testing. The genetic relatedness of the isolates was determined using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction ERIC-PCR technique. The isolates were also screened for the presence of both antimicrobial and plasmid-mediated quinolone resistance PMQR genes by PCR. Cefotaxime and ciprofloxacin co-resistant E. coli isolates with diverse genetic origins were recovered in 42.3 2252 of retail chicken carcasses. The E. coli isolates belonged to the phylogenetic group D2 were dominant 40.9, 922, followed by B1 27.3, 622, B23 18.2, 422, and A1 13.6, 322, respectively. Based on dendrogram analysis, the ERIC-PCR method differentiated the isolates into 10 clusters I-X. The multidrug resistance MDR was observed in 81.8 1822 of the isolates. PMQR determinants were not identified in any isolates tested. Molecular analysis revealed one or more β-lactamase-encoding genes in all isolates as a single or in combination: blaCTX-M-blaTEM n5, blaCMY-2 n5, blaCTX-M n5, blaCMY-2-blaSHV n3, blaCMY-2-blaTEM n3, and blaCTX-M-blaCMY-2 n1. This study highlights that retail chicken meat is an important reservoir of cefotaxime and ciprofloxacin co-resistant E. coli isolates. It is necessary to evaluate their contribution to the community and hospital infections.

 Antimikrobiyal dirençli bakterilerin gıda zinciri yoluyla insanlara bulaşması halk sağlığı açısından büyük önem taşımaktadır. Bu çalışmada Hatayda satışa sunulan perakende tavuk eti örneklerinde sefotaksim ve siprofloksasine dirençli Escherichia colinin izolasyonu ve karakterizasyonu amaçlandı. İzolatlar filogenetik grup tiplendirmesine ve antimikrobiyal duyarlılık testlerine tabi tutuldu. Ayrıca izolatlar arasındaki genetik yakınlığı belirlemek için Enterobacterial Repetitive Intergenic Consensus Polimeraz Zincir Reaksiyonu ERIC-PZR tekniği kullanıldı. Plazmit aracılı kinolon direnci PMQR ile diğer direnç genleri PCR ile araştırıldı. Perakende tavuk karkaslarının 42.3ünden 2252 farklı genotipe sahip sefotaksim ve siprofloksasine dirençli E. coli izole edildi. İzolatlar arasında dominant filogenetik grup D2 40.9, 922 olup; bunu sırasıyla B1 27.3, 622, B23 18.2, 422 ve A1 13.6, 322 filogrupları izledi. Dendrogram analizine dayalı olarak, ERIC-PCR yöntemi izolatları 10 kümeye I-X ayırdı. İzolatların 81.8inde 1822 çoklu ilaç direnci MDR belirlendi. PMQR genleri izolatların hiçbirinde tespit edilmezken, diğer sınıftan antimikrobiyallere dirence aracılık eden çok sayıda gen saptandı. İzolatlarda β-laktamaz sentezinden sorumlu genlerin tek veya kombine olarak bulunduğu görüldü: blaCTX-M-blaTEM n5, blaCMY-2 n5, blaCTX-M n5, blaCMY-2-blaSHV n3, blaCMY-2-blaTEM n3, and blaCTX-M-blaCMY-2 n1. Bu çalışma, perakende tavuk etinin, sefotaksim ve siprofloksasine dirençli E. coli izolatları için önemli bir rezervuar olduğunu göstermiştir. Toplum ve hastane enfeksiyonlarına katkılarının değerlendirilmesi gerekmektedir.

Makale detayları

Isolation and Characterization of Cefotaxime and Ciprofloxacin Co-Resistant Escherichia coli in Retail Chicken Carcasses

Yazarlar:

Özkan Aslantaş | Ahmet Murat Korkut | Mücella Bayırlı

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Mikrobiyolojisi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 2, pp.228-233

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304650

pISSN:

2146-7188

Antimicrobial resistance

Chicken carcasses

ERIC-PCR

Escherichia coli

Phylogenetic typingAntimikrobiyal direnç

ERIC-PCR

Escherichia coli

Filogenetik tiplendirme

Tavuk karkas

Sayı

Makale detayları

Yazarlar: Özkan Aslantaş | Ahmet Murat Korkut | Mücella Bayırlı

Yayıncı: Harran University

Ana Başlıklar: Veteriner Mikrobiyolojisi

Dergi: Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 2, pp.228-233

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001304650

pISSN: 2146-7188

Sayı

Yazarlar:

Özkan Aslantaş | Ahmet Murat Korkut | Mücella Bayırlı

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Mikrobiyolojisi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 2, pp.228-233

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304650

pISSN:

2146-7188