Mg deficiency changes the isoenzyme pattern of reactive oxygen species-relatedenzymes and regulates NADPH-oxidase-mediated ROS signaling in cotton

RENGİN ÖZGÜR UZİLDAY; BARIŞ UZİLDAY; TOLGA YALÇINKAYA; İSMAİL TÜRKAN

Makale detayları

Mg deficiency changes the isoenzyme pattern of reactive oxygen species-relatedenzymes and regulates NADPH-oxidase-mediated ROS signaling in cotton

Yazarlar:

RENGİN ÖZGÜR UZİLDAY | BARIŞ UZİLDAY | TOLGA YALÇINKAYA | İSMAİL TÜRKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.868-880

Belge Tipi:

Makale

Yayın Tarihi:

11/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811290

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

The aim of this work was to investigate changes in isoenzyme patterns of enzymes related to reactive oxygen species (ROS) detoxification such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione-S-transferase (GST) in cotton under Mg deficiency. Moreover, we aimed to elucidate how a ROS producer, NADPH oxidase (NOX), responds to changing Mg levels. Cotton plants were grown with different concentrations of MgSO4 in hydroponic medium to create nutrient deficiency (0, 75, 150, 1000 μM Mg). Gradual decreases in growth and photosynthetic rates were observed with declining Mg concentrations and 0, 75, and 150 μM Mg increased oxidative stress as evidenced by H2O2 and lipid peroxidation. Total activities of SOD, CAT, POX, APX, GR, and GST were increased while NOX activity was decreased with Mg deficiency. The activities of GR and GST were highest in plants treated with 0 μM Mg, indicating excess use of glutathione for redox regulation. The most striking results were the changes in isoenzyme patterns of SOD, NOX, POX, and GST. For example, a new Cu/ZnSOD isoenzyme was induced in plants treated with 0 μM Mg. Cotton plants adapt to Mg deficiency by changing the intensity of existing isoenzymes or inducing new ones.

Makale detayları

Mg deficiency changes the isoenzyme pattern of reactive oxygen species-relatedenzymes and regulates NADPH-oxidase-mediated ROS signaling in cotton

Yazarlar: RENGİN ÖZGÜR UZİLDAY | BARIŞ UZİLDAY | TOLGA YALÇINKAYA | İSMAİL TÜRKAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.868-880

Belge Tipi: Makale

Yayın Tarihi: 11/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000811290

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

RENGİN ÖZGÜR UZİLDAY | BARIŞ UZİLDAY | TOLGA YALÇINKAYA | İSMAİL TÜRKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.868-880

Belge Tipi:

Makale

Yayın Tarihi:

11/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811290

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152