Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Subclinical Bovine Mastitis

Özkan ASLANTAŞ; Cemil DEMİR; Hülya TÜRÜTOĞLU; Zafer CANTEKİN

Makale detayları

Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Subclinical Bovine Mastitis

Yazarlar:

Özkan ASLANTAŞ | Cemil DEMİR | Hülya TÜRÜTOĞLU | Zafer CANTEKİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2007 Vol.31 Issue 4, pp.253-257

Belge Tipi:

Makale

Yayın Tarihi:

3/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000768178

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.

 

This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.

Makale detayları

Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Subclinical Bovine Mastitis

Yazarlar:

Özkan ASLANTAŞ | Cemil DEMİR | Hülya TÜRÜTOĞLU | Zafer CANTEKİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2007 Vol.31 Issue 4, pp.253-257

Belge Tipi:

Makale

Yayın Tarihi:

3/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000768178

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Staphylococcus aureus

RFLP-PCR

mastitis

Sayı

Makale detayları

Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Subclinical Bovine Mastitis

Yazarlar: Özkan ASLANTAŞ | Cemil DEMİR | Hülya TÜRÜTOĞLU | Zafer CANTEKİN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2007 Vol.31 Issue 4, pp.253-257

Belge Tipi: Makale

Yayın Tarihi: 3/2007

Belge Dili: Türkçe

Document ID (Org No.): 2000768178

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Staphylococcus aureus

RFLP-PCR

mastitis

Sayı

Yazarlar:

Özkan ASLANTAŞ | Cemil DEMİR | Hülya TÜRÜTOĞLU | Zafer CANTEKİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2007 Vol.31 Issue 4, pp.253-257

Belge Tipi:

Makale

Yayın Tarihi:

3/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000768178

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128