The expression profile of aquaporin 1 in rat myocardium after severe burns

Wanli LI; Longying ZHA; Zhiqing LI; Yuesheng HUANG; Yiping ZHOU

Makale detayları

The expression profile of aquaporin 1 in rat myocardium after severe burns

Yazarlar:

Wanli LI | Longying ZHA | Zhiqing LI | Yuesheng HUANG | Yiping ZHOU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.481-488

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752640

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Background/aim: To investigate the myocardial expression profile of aquaporin 1 (AQP1) in rats after severe burns.   Materials and methods: Ninety healthy male adult Wistar rats were randomly assigned to the following treatments: sham operation (control group, n = 6), immediate fluid resuscitation treatment post scalding (IF group, n = 42), and delayed fluid resuscitation treatment after scalding (DF group, n = 42). At 3, 6, 12, 24, 48, 72, and 120 h after scalding, myocardial water contents were assayed and AQP1 expressions were detected by real-time polymerase chain reaction and western blot. The AQP levels in the myocardium at 12 h after scalding were assayed by gene microarrays.   Results: Scald injuries resulted in significantly synchronized increases in the myocardial water contents and the myocardial mRNA and protein expression of AQP1, with a peak at 12 h after scalding. Rats receiving delayed fluid resuscitation treatment had more severe myocardial edema and significantly higher myocardial AQP1 expressions than the rats receiving immediate fluid resuscitation treatment. The mRNAs of 6 other AQPs (AQP2, 3, 4, 6, 7, and 12b) were found to be changed in the myocardium among rats with different treatments.   Conclusion: AQP1 may play a functional role in the development of myocardial edema after scalding. Targeting AQP1 may provide opportunities for therapeutic intervention in myocardial edema following severe burns.

 

Background/aim: To investigate the myocardial expression profile of aquaporin 1 (AQP1) in rats after severe burns.   Materials and methods: Ninety healthy male adult Wistar rats were randomly assigned to the following treatments: sham operation (control group, n = 6), immediate fluid resuscitation treatment post scalding (IF group, n = 42), and delayed fluid resuscitation treatment after scalding (DF group, n = 42). At 3, 6, 12, 24, 48, 72, and 120 h after scalding, myocardial water contents were assayed and AQP1 expressions were detected by real-time polymerase chain reaction and western blot. The AQP levels in the myocardium at 12 h after scalding were assayed by gene microarrays.   Results: Scald injuries resulted in significantly synchronized increases in the myocardial water contents and the myocardial mRNA and protein expression of AQP1, with a peak at 12 h after scalding. Rats receiving delayed fluid resuscitation treatment had more severe myocardial edema and significantly higher myocardial AQP1 expressions than the rats receiving immediate fluid resuscitation treatment. The mRNAs of 6 other AQPs (AQP2, 3, 4, 6, 7, and 12b) were found to be changed in the myocardium among rats with different treatments.   Conclusion: AQP1 may play a functional role in the development of myocardial edema after scalding. Targeting AQP1 may provide opportunities for therapeutic intervention in myocardial edema following severe burns.

Makale detayları

The expression profile of aquaporin 1 in rat myocardium after severe burns

Yazarlar:

Wanli LI | Longying ZHA | Zhiqing LI | Yuesheng HUANG | Yiping ZHOU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.481-488

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752640

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Aquaporin 1

burn

myocardial edema

fluid resuscitation

Wistar rats

Sayı

Makale detayları

The expression profile of aquaporin 1 in rat myocardium after severe burns

Yazarlar: Wanli LI | Longying ZHA | Zhiqing LI | Yuesheng HUANG | Yiping ZHOU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.481-488

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000752640

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Aquaporin 1

burn

myocardial edema

fluid resuscitation

Wistar rats

Sayı

Yazarlar:

Wanli LI | Longying ZHA | Zhiqing LI | Yuesheng HUANG | Yiping ZHOU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.481-488

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752640

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144