Investigation of Campylobacter jejuni, Campylobacter coli and Campylobacter lari in Chicken Meat for Consumption with Real-Time PCR in Afyonkaraisar

Recep Kara

Makale detayları

Investigation of Campylobacter jejuni, Campylobacter coli and Campylobacter lari in Chicken Meat for Consumption with Real-Time PCR in Afyonkaraisar

Yazarlar:

Recep Kara

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Gıda Mühendisliği

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 1, pp.832-841

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284865

pISSN:

2687-3729

Anahtar kelimeler:

Özet:

 In todays World, the most commonly reported bacterial food diseases are caused by Campylobacter spp. According to researches, Campylobacter spp. can be found in broilers intestinal flora, also farm animals like sheep and cows intestinal flora provides suitable habitat for Campylobacter spp. In this study, Campylobacter jejuni, Campylobacter coli and Campylobacter lari were searched in broiler meat chicken breast, wing and legs which were sold between November 2020  February 2021 by using Real-Time PCR methodology. For this purpose, 30 chicken wings, 27 chicken legs, and 24 chicken breast, a total of 81 samples were analyzed. This research, it is determinded that 18  1581 of samples are contaminated by Campylobacter spp. C. jejuni was found in 13,33  430 of chicken wings, and and 7,41  227 of chicken legs but it was not found in any chicken breast samples. C. coli was found in 3,33  130 of chicken wings, 25,93  727 of chicken legs and 4,17  124 of chicken breasts. C. lari was not found in any of samples. Campylobacter spp infections, which are considered as zoonosis that threatens public health, are widespread in the world. Especially, due to the fact that Campylobacter jejuni is a regular member of the intestinal flora of poultry, hygiene rules during slaughter, especially in slaughterhouses; It is recommended to pay attention to cross-contamination.

 In todays World, the most commonly reported bacterial food diseases are caused by Campylobacter spp. According to researches, Campylobacter spp. can be found in broilers intestinal flora, also farm animals like sheep and cows intestinal flora provides suitable habitat for Campylobacter spp. In this study, Campylobacter jejuni, Campylobacter coli and Campylobacter lari were searched in broiler meat chicken breast, wing and legs which were sold between November 2020  February 2021 by using Real-Time PCR methodology. For this purpose, 30 chicken wings, 27 chicken legs, and 24 chicken breast, a total of 81 samples were analyzed. This research, it is determinded that 18  1581 of samples are contaminated by Campylobacter spp. C. jejuni was found in 13,33  430 of chicken wings, and and 7,41  227 of chicken legs but it was not found in any chicken breast samples. C. coli was found in 3,33  130 of chicken wings, 25,93  727 of chicken legs and 4,17  124 of chicken breasts. C. lari was not found in any of samples. Campylobacter spp infections, which are considered as zoonosis that threatens public health, are widespread in the world. Especially, due to the fact that Campylobacter jejuni is a regular member of the intestinal flora of poultry, hygiene rules during slaughter, especially in slaughterhouses; It is recommended to pay attention to cross-contamination.

 In todays World, the most commonly reported bacterial food diseases are caused by Campylobacter spp. According to researches, Campylobacter spp. can be found in broilers intestinal flora, also farm animals like sheep and cows intestinal flora provides suitable habitat for Campylobacter spp. In this study, Campylobacter jejuni, Campylobacter coli and Campylobacter lari were searched in broiler meat chicken breast, wing and legs which were sold between November 2020  February 2021 by using Real-Time PCR methodology. For this purpose, 30 chicken wings, 27 chicken legs, and 24 chicken breast, a total of 81 samples were analyzed. This research, it is determinded that 18  1581 of samples are contaminated by Campylobacter spp. C. jejuni was found in 13,33  430 of chicken wings, and and 7,41  227 of chicken legs but it was not found in any chicken breast samples. C. coli was found in 3,33  130 of chicken wings, 25,93  727 of chicken legs and 4,17  124 of chicken breasts. C. lari was not found in any of samples. Campylobacter spp infections, which are considered as zoonosis that threatens public health, are widespread in the world. Especially, due to the fact that Campylobacter jejuni is a regular member of the intestinal flora of poultry, hygiene rules during slaughter, especially in slaughterhouses; It is recommended to pay attention to cross-contamination.

 Dünyada gıda kaynaklı bakteriyel hastalıklar arasında en yaygını olarak rapor edilen Campylobacter spp.nın kanatlı barsak florasında yüksek düzeyde bulunmasının yanında, sığır ve koyun gibi çiftlik hayvanlarının barsak florası da uygun ortam oluşturmaktadır.Yapılan bu çalışmada Kasım 2020- Şubat 2021 tarihleri arasında Afyonkarahisar il merkezinde satışa sunulan piliç etlerinde but, göğüs ve kanat Campylobacter jejuni, Campylobacter coli ve Campylobacter lari suşları Real-Time PCR yöntemi ile araştırılmıştır. Bu amaçla 30 adet piliç kanat, 27 adet piliç but eti, 24 adet piliç göğüs eti örneği olmak üzere toplam 81 numune analize alınmıştır. Yapılan çalışmada tüm örneklerin 18,52 1581sinin Campylobacter ile kontamine olduğu tespit edilmiştir. C. jejuni, kanat etlerinde 13,33 430, but etlerinde 7,41 227 oranında saptanmış olup, göğüs etlerinde tespit edilmemiştir. C. coli kanat etlerinde 3,33 130, but etlerinde 25,93 727, göğüs etlerinde 4,17 124 seviyesinde tespit edilmiştir. C. lari, tüm piliç parça etlerinde kanat, göğüs ve but tespit edilmemiştir. Halk sağlığını tehdit eden önemli zoonozlar arasında yer alan Campylobacter spp enfeksiyonları tüm dünyada yaygın olarak görülmektedir. Özellikle Campylobacter jejuninin kanatlı hayvanlarının barsak florasının normal üyesi olması sebebiyle mezbahalarda kesim sırasında hijyen kurallarına ve çapraz kontaminasyona dikkat edilmesi önerilmektedir.

Makale detayları

Investigation of Campylobacter jejuni, Campylobacter coli and Campylobacter lari in Chicken Meat for Consumption with Real-Time PCR in Afyonkaraisar

Yazarlar:

Recep Kara

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Gıda Mühendisliği

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 1, pp.832-841

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284865

pISSN:

2687-3729

Campylobacter jejuni

C. coli

C. lari

chicken meat

Real-Time PCR

Campylobacter jejuni

C. coli

C. lari

Piliç Eti

Real-Time PCR

Sayı

Makale detayları

Yazarlar: Recep Kara

Yayıncı: Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar: Gıda Mühendisliği

Dergi: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 1, pp.832-841

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001284865

pISSN: 2687-3729

Sayı

Yazarlar:

Recep Kara

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Gıda Mühendisliği

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 1, pp.832-841

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284865

pISSN:

2687-3729