Comparison of endothelin and nitric oxide synthase blockers  on hemorheological parameters in endotoxemic rats

Neslihan Hacer Dikmenoğlu FALKMARKEN; Okan ARIHAN; Alper Bektaş İSKİT

Makale detayları

Comparison of endothelin and nitric oxide synthase blockers on hemorheological parameters in endotoxemic rats

Yazarlar:

Neslihan Hacer Dikmenoğlu FALKMARKEN | Okan ARIHAN | Alper Bektaş İSKİT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 3, pp.1045-1052

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751374

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: Septic shock is an important health problem that vastly alters cardiovascular and hemodynamic status. Increased production of nitric oxide (NO) and endothelin is a counterpart of this endotoxemic state. This study was conducted to test the hypothesis that nonselective NO synthesis blocker (L-NAME), inducible NO synthesis blocker (L-canavanine), or endothelin receptor antagonist (bosentan) will reverse the effects of sepsis on hemorheological parameters.   Materials and methods: Forty-eight male Sprague-Dawley rats were used in 8 groups: saline (control), endotoxin, bosentan, L-NAME, L-canavanine, endotoxin + bosentan, endotoxin + L-NAME, and endotoxin + L-canavanine. Blood was withdrawn at the 4th hour of endotoxemic state. Erythrocyte deformability and erythrocyte aggregation were determined by laser-assisted optical rotational cell analyzer at 37 °C. Plasma viscosity (mPa.s) was measured by a cone-plate viscometer with 0.5 mL of plasma.  Results: Endotoxin administration significantly increased aggregation half-time and lowered erythrocyte aggregation amplitude and aggregation index compared to the control, indicating a slower and weaker aggregation pattern. L-NAME and L-canavanine alleviated the effects of endotoxin on erythrocyte aggregation without altering the values in the control animals. However, bosentan did not perform such a restoration.   Conclusion: This finding suggests that these restoration effects of the blockers occur via their modulation of nitric oxide synthesis rather than through the endothelin pathway.

Makale detayları

Comparison of endothelin and nitric oxide synthase blockers on hemorheological parameters in endotoxemic rats

Yazarlar: Neslihan Hacer Dikmenoğlu FALKMARKEN | Okan ARIHAN | Alper Bektaş İSKİT

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2017 Vol.47 Issue 3, pp.1045-1052

Belge Tipi: Makale

Yayın Tarihi: 5/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000751374

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Neslihan Hacer Dikmenoğlu FALKMARKEN | Okan ARIHAN | Alper Bektaş İSKİT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 3, pp.1045-1052

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751374

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144