Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos

Tahir KARAŞAHİN; Numan AKYOL; Muharrem SATILMIŞ

Makale detayları

Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos

Yazarlar:

Tahir KARAŞAHİN | Numan AKYOL | Muharrem SATILMIŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 3, pp.253-256

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000765985

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The objective of this study was to investigate the conception rates achieved with the transfer of in-vivo–derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Seven-day-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed embryos were 30.4% (7/23) and 42.9% (9/21), respectively. The difference between conception rates achieved in the 2 groups was not statistically significant (P > 0.05). For the sexed embryos the conception rates achieved with the transfer of male and female embryos were 27.2% (3/11) and 33.3% (4/12), respectively. The accuracy of embryo sexing with nonelectrophoretic PCR was 66.6% for male embryos and 100% for female embryos postdelivery. The mean rate of accuracy determined for embryo sexing at the end of the study was 83.33%.

 

The objective of this study was to investigate the conception rates achieved with the transfer of in-vivo–derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Seven-day-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed embryos were 30.4% (7/23) and 42.9% (9/21), respectively. The difference between conception rates achieved in the 2 groups was not statistically significant (P > 0.05). For the sexed embryos the conception rates achieved with the transfer of male and female embryos were 27.2% (3/11) and 33.3% (4/12), respectively. The accuracy of embryo sexing with nonelectrophoretic PCR was 66.6% for male embryos and 100% for female embryos postdelivery. The mean rate of accuracy determined for embryo sexing at the end of the study was 83.33%.

Makale detayları

Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos

Yazarlar:

Tahir KARAŞAHİN | Numan AKYOL | Muharrem SATILMIŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 3, pp.253-256

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000765985

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Bovine embryo sexing

PCR

vitrification

transfer

Sayı

Makale detayları

Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos

Yazarlar: Tahir KARAŞAHİN | Numan AKYOL | Muharrem SATILMIŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 3, pp.253-256

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000765985

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Bovine embryo sexing

PCR

vitrification

transfer

Sayı

Yazarlar:

Tahir KARAŞAHİN | Numan AKYOL | Muharrem SATILMIŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 3, pp.253-256

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000765985

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128