HIGH PREVALENCE OF TRANSFERABLE INTEGRON-ASSOCIATED DRUG RESISTANCE IN ESCHERICHIA COLI STRAINS ISOLATED FROM BLOOD CULTURES IN A UNIVERSITY HOSPITAL IN TRABZON, TURKIYE

Ahu Reis; Osman Birol Özgümüş; Erva Rakıcı; İnci Durukan; Kurtuluş Buruk; Gülçin Bayramoğlu; Ali Osman Kılıç

Makale detayları

HIGH PREVALENCE OF TRANSFERABLE INTEGRON-ASSOCIATED DRUG RESISTANCE IN ESCHERICHIA COLI STRAINS ISOLATED FROM BLOOD CULTURES IN A UNIVERSITY HOSPITAL IN TRABZON, TURKIYE

Yazarlar:

Ahu Reis | Osman Birol Özgümüş | Erva Rakıcı | İnci Durukan | Kurtuluş Buruk | Gülçin Bayramoğlu | Ali Osman Kılıç

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Clinical Microbiology

Dergi:

Meandros Medical And Dental Journal Vol.25 Issue 4, pp.436-450

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334514

pISSN:

2149-9063

Anahtar kelimeler:

Özet:

 Objective: This study aimed to determine the carriage of transferable integron-associated drug resistance in Escherichia coli E. coli strains isolated from blood cultures. Materials and Methods: A total of 111 E. coli isolates were included in this study. Antimicrobial susceptibility testing of the isolates against 17 antibiotics was performed using an automated microbiology system. Integron-specific polymerase chain reactions PCR were used to detect the presence of integrons. The antibiotic resistance gene cassettes in the variable regions of integrons were analyzed by DNA sequencing. Plasmid transfer assays were performed using the broth mating method. The clonal relationships among integron-carrying strains were evaluated by pulsed-field gel electrophoresis PFGE. Results: Resistance rates to antibiotics ranged from 0.9 to 63. Thirty-eight strains carried gene cassettes encoding dfrA7, dfr17-aadA5, dfrV, dfrA1-aadA1, and dfrA12-aadA2. Seven strains possessed class 2 integrons with gene arrays dfrA1-sat2-aadA1 and dfrA1-sat2-aadA30. Twenty-two integron-carrying isolates harbored conjugative resistance plasmids, three of which were identified as belonging to the IncN group. Two strains with class 1 integrons, isolated from different clinics, exhibited similar patterns in the PFGE analysis. Conclusion: Approximately 50 of E. coli isolates from blood cultures at our hospital were found to carry integron-associated transferable drug resistance, suggesting their potential role in the horizontal dissemination of resistance genes. Further research is needed to understand the prevalence of E. coli strains of blood origin and the role of integrons and gene cassette arrays in the spread of resistance.

 Objective: This study aimed to determine the carriage of transferable integron-associated drug resistance in Escherichia coli E. coli strains isolated from blood cultures. Materials and Methods: A total of 111 E. coli isolates were included in this study. Antimicrobial susceptibility testing of the isolates against 17 antibiotics was performed using an automated microbiology system. Integron-specific polymerase chain reactions PCR were used to detect the presence of integrons. The antibiotic resistance gene cassettes in the variable regions of integrons were analyzed by DNA sequencing. Plasmid transfer assays were performed using the broth mating method. The clonal relationships among integron-carrying strains were evaluated by pulsed-field gel electrophoresis PFGE. Results: Resistance rates to antibiotics ranged from 0.9 to 63. Thirty-eight strains carried gene cassettes encoding dfrA7, dfr17-aadA5, dfrV, dfrA1-aadA1, and dfrA12-aadA2. Seven strains possessed class 2 integrons with gene arrays dfrA1-sat2-aadA1 and dfrA1-sat2-aadA30. Twenty-two integron-carrying isolates harbored conjugative resistance plasmids, three of which were identified as belonging to the IncN group. Two strains with class 1 integrons, isolated from different clinics, exhibited similar patterns in the PFGE analysis. Conclusion: Approximately 50 of E. coli isolates from blood cultures at our hospital were found to carry integron-associated transferable drug resistance, suggesting their potential role in the horizontal dissemination of resistance genes. Further research is needed to understand the prevalence of E. coli strains of blood origin and the role of integrons and gene cassette arrays in the spread of resistance.

 Objective: This study aimed to determine the carriage of transferable integron-associated drug resistance in Escherichia coli E. coli strains isolated from blood cultures. Materials and Methods: A total of 111 E. coli isolates were included in this study. Antimicrobial susceptibility testing of the isolates against 17 antibiotics was performed using an automated microbiology system. Integron-specific polymerase chain reactions PCR were used to detect the presence of integrons. The antibiotic resistance gene cassettes in the variable regions of integrons were analyzed by DNA sequencing. Plasmid transfer assays were performed using the broth mating method. The clonal relationships among integron-carrying strains were evaluated by pulsed-field gel electrophoresis PFGE. Results: Resistance rates to antibiotics ranged from 0.9 to 63. Thirty-eight strains carried gene cassettes encoding dfrA7, dfr17-aadA5, dfrV, dfrA1-aadA1, and dfrA12-aadA2. Seven strains possessed class 2 integrons with gene arrays dfrA1-sat2-aadA1 and dfrA1-sat2-aadA30. Twenty-two integron-carrying isolates harbored conjugative resistance plasmids, three of which were identified as belonging to the IncN group. Two strains with class 1 integrons, isolated from different clinics, exhibited similar patterns in the PFGE analysis. Conclusion: Approximately 50 of E. coli isolates from blood cultures at our hospital were found to carry integron-associated transferable drug resistance, suggesting their potential role in the horizontal dissemination of resistance genes. Further research is needed to understand the prevalence of E. coli strains of blood origin and the role of integrons and gene cassette arrays in the spread of resistance.

 Giriş: Bu çalışmada, Karadeniz Teknik Üniversitesi Hastanesi Araştırma ve Uygulama Merkezinde kan kültürlerinden izole edilen Escherichia coli E. coli suşlarının transfer edilebilir integron ilişkili ilaç direncine sahip olup olmadığının belirlenmesi amaçlanmıştır. Materyal ve Metod: İntegronların varlığı, integrona özgü polimeraz zincir reaksiyonları ile belirlendi ve amplikonlar, DNA dizilimi ile analiz edildi. Plazmid transfer deneyleri, sıvıda çiftleşme yöntemiyle gerçekleştirildi. İntegron içeren suşların klonal ilişkisi pulsed field jel elektroforezi PFGE ile değerlendirildi. Bulgular: Kullanılan antibiyotik gruplarına direnç oranları 0,9 ile 63 arasındaydı. Otuz sekiz 34,2 suş, dfrA7, dfr17-aadA5, dfrV, dfrA1-aadA1 ve dfrA12-aadA2 gen kaset dizilerini taşıyan sınıf 1 integronları içeriyordu. Yedi 6,3 suş, dfrA1-sat2-aadA1 ve dfrA1-sat2-aadA30 gen kaset dizilerine sahip sınıf 2 integronları içeriyordu. İntegron taşıyan izolatlarda 22 konjugatif direnç plazmidi barındırıldı ve bunlardan üçünün IncN grubunda olduğu belirlendi. Farklı kliniklerden izole edilen sınıf 1 integronları taşıyan dört suş, PFGE analizinde klonal olarak benzer modeller gösterdi. Sonuç: Hastanemizdeki E. coli izolatlarının yaklaşık 50sinin integronla ilişkili aktarılabilir ilaç direnci taşıdığı ve yatay yayılımda önemli bir rol oynayabileceği sonucuna vardık. Bu hastanede ve ülke genelinde kan kökenli E. coli suşlarında integronların taşınması arasındaki ilişkinin açıklanabilmesi için daha ileri moleküler epidemiyolojik araştırmalara ihtiyaç vardır.

Makale detayları

HIGH PREVALENCE OF TRANSFERABLE INTEGRON-ASSOCIATED DRUG RESISTANCE IN ESCHERICHIA COLI STRAINS ISOLATED FROM BLOOD CULTURES IN A UNIVERSITY HOSPITAL IN TRABZON, TURKIYE

Yazarlar:

Ahu Reis | Osman Birol Özgümüş | Erva Rakıcı | İnci Durukan | Kurtuluş Buruk | Gülçin Bayramoğlu | Ali Osman Kılıç

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Clinical Microbiology

Dergi:

Meandros Medical And Dental Journal Vol.25 Issue 4, pp.436-450

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334514

pISSN:

2149-9063

Escherichia coli

Gene cassette

Integron

Conjugation

PlasmidEscherichia coli

Gen kaset

İntegron

Konjugasyon

Plazmid

Sayı

Makale detayları

Yazarlar: Ahu Reis | Osman Birol Özgümüş | Erva Rakıcı | İnci Durukan | Kurtuluş Buruk | Gülçin Bayramoğlu | Ali Osman Kılıç

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Clinical Microbiology

Dergi: Meandros Medical And Dental Journal Vol.25 Issue 4, pp.436-450

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001334514

pISSN: 2149-9063

Sayı

Yazarlar:

Ahu Reis | Osman Birol Özgümüş | Erva Rakıcı | İnci Durukan | Kurtuluş Buruk | Gülçin Bayramoğlu | Ali Osman Kılıç

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Clinical Microbiology

Dergi:

Meandros Medical And Dental Journal Vol.25 Issue 4, pp.436-450

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334514

pISSN:

2149-9063