Effects of H+-K+ ATPase Inhibitors (Omeprazole and Lansoprazole) on Fertilization-Induced Bioelectrical Potential Changes in the Egg of the Frog, Rana cameranoi

Şeref ERDOĞAN; Kerem Tuncay ÖZGÜNEN; Tuncay ÖZGÜNEN

Makale detayları

Effects of H+-K+ ATPase Inhibitors (Omeprazole and Lansoprazole) on Fertilization-Induced Bioelectrical Potential Changes in the Egg of the Frog, Rana cameranoi

Yazarlar:

Şeref ERDOĞAN | Kerem Tuncay ÖZGÜNEN | Tuncay ÖZGÜNEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 2, pp.329-336

Belge Tipi:

Makale

Yayın Tarihi:

1/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769588

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Fertilization triggers Ca2+ increase and alkalization in the eggs of some species such as the frog. Ooplasmic Ca2+ increase triggers fertilization potential (FP), and alkalization can have a permissive effect in Ca2+ increase. It can be expected that pH regulation in the egg can affect both ooplasmic Ca2+ levels and the events triggered by increasing Ca2+. The evidence of the possibility of H+-K+ ATPase in different tissues in recent studies prompted us to wonder about the probability of the existence of this pump in the egg. Therefore, we decided to investigate the presence of H+-K+ ATPase by bioelectrical potential recordings in frog eggs. Thus, frog eggs were inseminated while the resting membrane potential (RMP) in the solution containing different concentrations (0.3, 0.6, 0.9 and 1.2 mM) of pump inhibitors (omeprazole and lansoprazole) was recorded. Evidence of H+-K+ ATPase was sought from the effects of these pump inhibitors on FP variables.  While omeprazole had a significant effect on RMP and FP variables at a 1.2 mM concentration alone, lansoprazole had significant effects at concentrations higher than 0.6 mM in the frog eggs. It was determined that the effects of the 2 inhibitors were similar.  In conclusion, H+-K+ ATPase may be present in frog eggs due to the similar effects of both pump inhibitors on the potential change triggered by fertilization. Thus, this pump might have a function in alkalization triggered by fertilization in the frog egg.

 

Fertilization triggers Ca2+ increase and alkalization in the eggs of some species such as the frog. Ooplasmic Ca2+ increase triggers fertilization potential (FP), and alkalization can have a permissive effect in Ca2+ increase. It can be expected that pH regulation in the egg can affect both ooplasmic Ca2+ levels and the events triggered by increasing Ca2+. The evidence of the possibility of H+-K+ ATPase in different tissues in recent studies prompted us to wonder about the probability of the existence of this pump in the egg. Therefore, we decided to investigate the presence of H+-K+ ATPase by bioelectrical potential recordings in frog eggs. Thus, frog eggs were inseminated while the resting membrane potential (RMP) in the solution containing different concentrations (0.3, 0.6, 0.9 and 1.2 mM) of pump inhibitors (omeprazole and lansoprazole) was recorded. Evidence of H+-K+ ATPase was sought from the effects of these pump inhibitors on FP variables.  While omeprazole had a significant effect on RMP and FP variables at a 1.2 mM concentration alone, lansoprazole had significant effects at concentrations higher than 0.6 mM in the frog eggs. It was determined that the effects of the 2 inhibitors were similar.  In conclusion, H+-K+ ATPase may be present in frog eggs due to the similar effects of both pump inhibitors on the potential change triggered by fertilization. Thus, this pump might have a function in alkalization triggered by fertilization in the frog egg.

Makale detayları

Effects of H+-K+ ATPase Inhibitors (Omeprazole and Lansoprazole) on Fertilization-Induced Bioelectrical Potential Changes in the Egg of the Frog, Rana cameranoi

Yazarlar:

Şeref ERDOĞAN | Kerem Tuncay ÖZGÜNEN | Tuncay ÖZGÜNEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 2, pp.329-336

Belge Tipi:

Makale

Yayın Tarihi:

1/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769588

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Frog egg

H+-K+ ATPase

bioelectrical potentials

omeprazole

lansoprazole

Sayı

Makale detayları

Effects of H+-K+ ATPase Inhibitors (Omeprazole and Lansoprazole) on Fertilization-Induced Bioelectrical Potential Changes in the Egg of the Frog, Rana cameranoi

Yazarlar: Şeref ERDOĞAN | Kerem Tuncay ÖZGÜNEN | Tuncay ÖZGÜNEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 2, pp.329-336

Belge Tipi: Makale

Yayın Tarihi: 1/2004

Belge Dili: Türkçe

Document ID (Org No.): 2000769588

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Frog egg

H+-K+ ATPase

bioelectrical potentials

omeprazole

lansoprazole

Sayı

Yazarlar:

Şeref ERDOĞAN | Kerem Tuncay ÖZGÜNEN | Tuncay ÖZGÜNEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 2, pp.329-336

Belge Tipi:

Makale

Yayın Tarihi:

1/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769588

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128