Investigation of Methylation-Related Expression Changes of GABRB3 Gene in Oral Malignant Lesions

Seval Turna; Ahmet Özkan; Erdinç Uysal; Murat Ulusan; Leyla Khalilova; Nejat Vakur Olgaç; Semra Demokan

Makale detayları

Investigation of Methylation-Related Expression Changes of GABRB3 Gene in Oral Malignant Lesions

Yazarlar:

Seval Turna | Ahmet Özkan | Erdinç Uysal | Murat Ulusan | Leyla Khalilova | Nejat Vakur Olgaç | Semra Demokan

Yayıncı:

Giresun University

Ana Başlıklar:

Öngörücü ve Prognostik Belirteçler

Dergi:

Doğu Karadeniz Sağlık Bilimleri Dergisi Vol.4 Issue 3, pp.272-279

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349986

pISSN:

2822-6445

Anahtar kelimeler:

Özet:

 Aim: Oral cancer constitutes 2-4 of all cancers and is the most common cancer in the head and neck region after laryngeal cancer. Oral squamous cell carcinoma OSCC constitutes more than 90 of all oral cavity carcinomas. While the 5-year survival rate in OSCC is 40, this rate increases to 95 in early diagnosis. The GABRB3 gene was identified as a potential epigenetic biomarker candidate in the TUBITAK-SBAG-114S497 project conducted by Demokan et al., and in this study, the differing methylation and expression levels in OSCC patients were examined and its biomarker potential in early diagnosis and prognosis was investigated. Methods: DNA and RNA were isolated from tissue samples taken from 15 patients diagnosed with OSCC, then the methylation status and gene expression levels of the GABRB3 gene were evaluated by Quantitative Methylation Specific PCR and Real-Time PCR methods, respectively. Results and Conclusions: Methylation was detected in the promoter region of the GABRB3 gene in 60 of tumor samples from OSCC patients. When expression levels were evaluated, loss of expression of the GABRB3 gene was detected in 46.7 of samples compared to normal tissues in tumor tissues, while increased expression was observed as 13.3. Methylation was detected in all patients with decreased expression levels. While decreased expression was observed in 33.3 of tumor tissues having no methylation, increased expression was observed in 50. It is thought that GABRB3 gene may be responsible for a special subgroup of OSCC patients via methylation-related loss of expression and can be used as a potential biomarker candidate in early diagnosis and prognosis determination. Further studies in larger patient groups are needed to confirm these findings.

 Aim: Oral cancer constitutes 2-4 of all cancers and is the most common cancer in the head and neck region after laryngeal cancer. Oral squamous cell carcinoma OSCC constitutes more than 90 of all oral cavity carcinomas. While the 5-year survival rate in OSCC is 40, this rate increases to 95 in early diagnosis. The GABRB3 gene was identified as a potential epigenetic biomarker candidate in the TUBITAK-SBAG-114S497 project conducted by Demokan et al., and in this study, the differing methylation and expression levels in OSCC patients were examined and its biomarker potential in early diagnosis and prognosis was investigated. Methods: DNA and RNA were isolated from tissue samples taken from 15 patients diagnosed with OSCC, then the methylation status and gene expression levels of the GABRB3 gene were evaluated by Quantitative Methylation Specific PCR and Real-Time PCR methods, respectively. Results and Conclusions: Methylation was detected in the promoter region of the GABRB3 gene in 60 of tumor samples from OSCC patients. When expression levels were evaluated, loss of expression of the GABRB3 gene was detected in 46.7 of samples compared to normal tissues in tumor tissues, while increased expression was observed as 13.3. Methylation was detected in all patients with decreased expression levels. While decreased expression was observed in 33.3 of tumor tissues having no methylation, increased expression was observed in 50. It is thought that GABRB3 gene may be responsible for a special subgroup of OSCC patients via methylation-related loss of expression and can be used as a potential biomarker candidate in early diagnosis and prognosis determination. Further studies in larger patient groups are needed to confirm these findings.

 Aim: Oral cancer constitutes 2-4 of all cancers and is the most common cancer in the head and neck region after laryngeal cancer. Oral squamous cell carcinoma OSCC constitutes more than 90 of all oral cavity carcinomas. While the 5-year survival rate in OSCC is 40, this rate increases to 95 in early diagnosis. The GABRB3 gene was identified as a potential epigenetic biomarker candidate in the TUBITAK-SBAG-114S497 project conducted by Demokan et al., and in this study, the differing methylation and expression levels in OSCC patients were examined and its biomarker potential in early diagnosis and prognosis was investigated. Methods: DNA and RNA were isolated from tissue samples taken from 15 patients diagnosed with OSCC, then the methylation status and gene expression levels of the GABRB3 gene were evaluated by Quantitative Methylation Specific PCR and Real-Time PCR methods, respectively. Results and Conclusions: Methylation was detected in the promoter region of the GABRB3 gene in 60 of tumor samples from OSCC patients. When expression levels were evaluated, loss of expression of the GABRB3 gene was detected in 46.7 of samples compared to normal tissues in tumor tissues, while increased expression was observed as 13.3. Methylation was detected in all patients with decreased expression levels. While decreased expression was observed in 33.3 of tumor tissues having no methylation, increased expression was observed in 50. It is thought that GABRB3 gene may be responsible for a special subgroup of OSCC patients via methylation-related loss of expression and can be used as a potential biomarker candidate in early diagnosis and prognosis determination. Further studies in larger patient groups are needed to confirm these findings.

 Amaç: Oral kanserler, tüm kanserlerin 2-4ünü oluşturur ve baş-boyun bölgesinde larenks kanserinden sonra en sık görülen kanser türüdür. Oral skuamöz hücreli karsinom OSCC, tüm ağız boşluğu karsinomlarının 90ından fazlasını oluşturur. OSCCde 5 yıllık sağkalım oranı 40 iken, erken tanıda bu oran 95e çıkmaktadır. GABRB3 geni, Demokan ve arkadaşları tarafından tamamlanmış TÜBİTAK-SBAG-114S497 kodlu projede potansiyel bir epigenetik biyobelirteç adayı olarak tanımlanmış ve bu çalışmada, OSCC hastalarındaki farklı metilasyon ve ekspresyon seviyeleri incelenerek erken tanı ve prognozdaki biyobelirteç potansiyeli araştırılmıştır. Yöntem: OSCC tanısı almış 15 hastadan alınan doku örneklerinden DNA ve RNA izole edildi, ardından GABRB3 geni metilasyon durumu ve gen ekspresyon seviyeleri sırasıyla Kantitatif Metilasyon Spesifik PCR ve Gerçek Zamanlı PCR yöntemleri ile değerlendirildi. Bulgular ve Sonuçlar: OSCC hastalarından alınan tümör örneklerinin 60ında GABRB3 geni promotör bölgesinde metilasyon tespit edildi. Ekspresyon seviyeleri değerlendirildiğinde, tümör dokularında normal dokulara kıyasla örneklerin 46,7sinde GABRB3 gen ekspresyon kaybı tespit edilirken, 13,3ünde ekspresyon artışı gözlendi. Ekspresyon seviyeleri azalmış olan tüm hastalarda metilasyon tespit edildi. Metilasyon gözlenmeyen tümör dokularının 33,3ünde ekspresyonda azalma gözlenirken, 50sinde ekspresyon artışı gözlendi. GABRB3 geni metilasyona bağlı ekspresyon kaybı yoluyla OSCC hastalarının özel bir alt grubundan sorumlu olabileceği ve erken tanı ve prognozun belirlenmesinde potansiyel bir biyobelirteç adayı olarak kullanılabileceği düşünülmektedir. Bu bulguları doğrulamak için daha geniş hasta gruplarıyla yapılacak ileri çalışmalara ihtiyaç vardır.

Makale detayları

Investigation of Methylation-Related Expression Changes of GABRB3 Gene in Oral Malignant Lesions

Yazarlar:

Seval Turna | Ahmet Özkan | Erdinç Uysal | Murat Ulusan | Leyla Khalilova | Nejat Vakur Olgaç | Semra Demokan

Yayıncı:

Giresun University

Ana Başlıklar:

Öngörücü ve Prognostik Belirteçler

Dergi:

Doğu Karadeniz Sağlık Bilimleri Dergisi Vol.4 Issue 3, pp.272-279

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349986

pISSN:

2822-6445

Oral squamous cell carcinoma

methylation

expression

biomarkerOral skuamöz hücreli karsinom

metilasyon

ekspresyon

biyobelirteç

Sayı

Makale detayları

Yazarlar: Seval Turna | Ahmet Özkan | Erdinç Uysal | Murat Ulusan | Leyla Khalilova | Nejat Vakur Olgaç | Semra Demokan

Yayıncı: Giresun University

Ana Başlıklar: Öngörücü ve Prognostik Belirteçler

Dergi: Doğu Karadeniz Sağlık Bilimleri Dergisi Vol.4 Issue 3, pp.272-279

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001349986

pISSN: 2822-6445

Sayı

Yazarlar:

Seval Turna | Ahmet Özkan | Erdinç Uysal | Murat Ulusan | Leyla Khalilova | Nejat Vakur Olgaç | Semra Demokan

Yayıncı:

Giresun University

Ana Başlıklar:

Öngörücü ve Prognostik Belirteçler

Dergi:

Doğu Karadeniz Sağlık Bilimleri Dergisi Vol.4 Issue 3, pp.272-279

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349986

pISSN:

2822-6445